Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers
Staphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate VacR, which included recombinant S. aureus protein clumping factor A (rClf...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S1090023318300686 http://hdl.handle.net/20.500.12123/2694 https://doi.org/10.1016/j.tvjl.2018.03.005 |
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| author | Pujato, Nazarena Camussone, Cecilia Renna, María Sol Perrig, Melina Soledad Morein, Bror Calvinho, Luis Fernando Marcipar, Iván Sergio |
| author_browse | Calvinho, Luis Fernando Camussone, Cecilia Marcipar, Iván Sergio Morein, Bror Perrig, Melina Soledad Pujato, Nazarena Renna, María Sol |
| author_facet | Pujato, Nazarena Camussone, Cecilia Renna, María Sol Perrig, Melina Soledad Morein, Bror Calvinho, Luis Fernando Marcipar, Iván Sergio |
| author_sort | Pujato, Nazarena |
| collection | INTA Digital |
| description | Staphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate VacR, which included recombinant S. aureus protein clumping factor A (rClf), fibronectin binding protein A (rFnBP) and hemolysin beta (rBt), formulated with a novel immune-stimulating complex. Comparisons were made between healthy pregnant heifers that received either VacR (n = 8; VacR group) or phosphate buffered saline (PBS) plus adjuvant (control group) SC in the supramammary lymph node area on days 45 and 15 before the expected calving date. Blood and foremilk samples were collected from 7 to 60 days post-calving.
After calving, heifers in the VacR group produced higher total IgG (IgGtotal) titers against each component, in both serum (rBt, 3.4 × 105; rClf, 3.1 × 105; rFnBP, 2.3 × 105) and milk (rBt, 2.6 × 104; rClf, 1.3 × 104; rFnBP, 1.1 × 104), than control heifers (P < 0.0001). There were increased concentrations of IgG1 and IgG2 in VacR group (P < 0.05), in both serum and milk. Humoral responses remained high throughout the period most susceptible to intramammary infections (P < 0.01). Antibodies produced against S. aureus rClf and rFnBP reduced bacterial adherence to fibronectin and fibrinogen by 73% and 67%, respectively (P < 0.001). Milk antibodies against these adhesins inhibited S. aureus invasion of a mammary epithelial cell line (MAC-T), resulting in 15.7% of bacteria internalized (P < 0.0001). There was an approximately 6-fold reduction in the hemolysis titer for the native hemolysin in the VacR group compared to the control group (P < 0.0001) and a significantly increase in the proportion of positive neutrophils (VacR, 29.7%; PBS, 13.1%) and the mean fluorescent index (VacR, 217.4; PBS, 152.6; P < 0.01) in the VacR group. The results suggest that VacR is a valuable vaccine candidate against S. aureus infections, and merits further field trials and experimental challenges. |
| format | Artículo |
| id | INTA2694 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26942018-07-20T11:50:33Z Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers Pujato, Nazarena Camussone, Cecilia Renna, María Sol Perrig, Melina Soledad Morein, Bror Calvinho, Luis Fernando Marcipar, Iván Sergio Ganado Bovino Novilla Respuesta Inmunológica Vacuna Staphylococcus aureus Mastítis Bovina Gestación Cattle Heifers Immune Response Vaccines Bovine Mastitis Pregnancy Staphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate VacR, which included recombinant S. aureus protein clumping factor A (rClf), fibronectin binding protein A (rFnBP) and hemolysin beta (rBt), formulated with a novel immune-stimulating complex. Comparisons were made between healthy pregnant heifers that received either VacR (n = 8; VacR group) or phosphate buffered saline (PBS) plus adjuvant (control group) SC in the supramammary lymph node area on days 45 and 15 before the expected calving date. Blood and foremilk samples were collected from 7 to 60 days post-calving. After calving, heifers in the VacR group produced higher total IgG (IgGtotal) titers against each component, in both serum (rBt, 3.4 × 105; rClf, 3.1 × 105; rFnBP, 2.3 × 105) and milk (rBt, 2.6 × 104; rClf, 1.3 × 104; rFnBP, 1.1 × 104), than control heifers (P < 0.0001). There were increased concentrations of IgG1 and IgG2 in VacR group (P < 0.05), in both serum and milk. Humoral responses remained high throughout the period most susceptible to intramammary infections (P < 0.01). Antibodies produced against S. aureus rClf and rFnBP reduced bacterial adherence to fibronectin and fibrinogen by 73% and 67%, respectively (P < 0.001). Milk antibodies against these adhesins inhibited S. aureus invasion of a mammary epithelial cell line (MAC-T), resulting in 15.7% of bacteria internalized (P < 0.0001). There was an approximately 6-fold reduction in the hemolysis titer for the native hemolysin in the VacR group compared to the control group (P < 0.0001) and a significantly increase in the proportion of positive neutrophils (VacR, 29.7%; PBS, 13.1%) and the mean fluorescent index (VacR, 217.4; PBS, 152.6; P < 0.01) in the VacR group. The results suggest that VacR is a valuable vaccine candidate against S. aureus infections, and merits further field trials and experimental challenges. EEA Rafaela Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Perrig, Melina Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Fil: Morein, Bror. Uppsala University. Department of Clinical Virology; Suecia Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Marcipar, Iván Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina 2018-06-29T11:49:39Z 2018-06-29T11:49:39Z 2018-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1090023318300686 http://hdl.handle.net/20.500.12123/2694 1090-0233 https://doi.org/10.1016/j.tvjl.2018.03.005 eng info:eu-repo/semantics/restrictedAccess application/pdf The Veterinary Journal 235 : 47-53 (May 2018) |
| spellingShingle | Ganado Bovino Novilla Respuesta Inmunológica Vacuna Staphylococcus aureus Mastítis Bovina Gestación Cattle Heifers Immune Response Vaccines Bovine Mastitis Pregnancy Pujato, Nazarena Camussone, Cecilia Renna, María Sol Perrig, Melina Soledad Morein, Bror Calvinho, Luis Fernando Marcipar, Iván Sergio Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers |
| title | Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers |
| title_full | Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers |
| title_fullStr | Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers |
| title_full_unstemmed | Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers |
| title_short | Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers |
| title_sort | evaluation of the humoral immune response to a multicomponent recombinant vaccine against s aureus in healthy pregnant heifers |
| topic | Ganado Bovino Novilla Respuesta Inmunológica Vacuna Staphylococcus aureus Mastítis Bovina Gestación Cattle Heifers Immune Response Vaccines Bovine Mastitis Pregnancy |
| url | https://www.sciencedirect.com/science/article/pii/S1090023318300686 http://hdl.handle.net/20.500.12123/2694 https://doi.org/10.1016/j.tvjl.2018.03.005 |
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