Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2688 https://www.sciencedirect.com/science/article/pii/S0378113514003344?via%3Dihub https://doi.org/10.1016/j.vetmic.2014.07.011 |
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| author | Martínez, Diana Thompson, Carolina Soledad Draghi, Maria Graciela Canavesio, Vilma Jacobo, Roberto Zimmer, Patricia Andrea Elena, Sebastián Alejandro Nicola, Ana M. Torioni, Susana Marta |
| author_browse | Canavesio, Vilma Draghi, Maria Graciela Elena, Sebastián Alejandro Jacobo, Roberto Martínez, Diana Nicola, Ana M. Thompson, Carolina Soledad Torioni, Susana Marta Zimmer, Patricia Andrea |
| author_facet | Martínez, Diana Thompson, Carolina Soledad Draghi, Maria Graciela Canavesio, Vilma Jacobo, Roberto Zimmer, Patricia Andrea Elena, Sebastián Alejandro Nicola, Ana M. Torioni, Susana Marta |
| author_sort | Martínez, Diana |
| collection | INTA Digital |
| description | An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus–B. melitensis–B. ovis–B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas. |
| format | Artículo |
| id | INTA2688 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26882023-01-13T15:06:40Z Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas Martínez, Diana Thompson, Carolina Soledad Draghi, Maria Graciela Canavesio, Vilma Jacobo, Roberto Zimmer, Patricia Andrea Elena, Sebastián Alejandro Nicola, Ana M. Torioni, Susana Marta Búfalo de Agua Brucella Abortus Genotipos Fenotipos Brucelosis Water Buffaloes Genotypes Phenotypes Brucellosis América Latina An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus–B. melitensis–B. ovis–B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas. EEA Rafaela Fil: Martinez, Diana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Draghi, Maria Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Canavesio, Vilma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Jacobo, Roberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Elena, Sebastián. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentina Fil: Nicola, Ana M. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentina Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina 2018-06-28T13:59:03Z 2018-06-28T13:59:03Z 2014-09-17 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2688 https://www.sciencedirect.com/science/article/pii/S0378113514003344?via%3Dihub 0378-1135 1873-2542 https://doi.org/10.1016/j.vetmic.2014.07.011 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary Microbiology 173 (1–2) : 172-176 (September 2014) |
| spellingShingle | Búfalo de Agua Brucella Abortus Genotipos Fenotipos Brucelosis Water Buffaloes Genotypes Phenotypes Brucellosis América Latina Martínez, Diana Thompson, Carolina Soledad Draghi, Maria Graciela Canavesio, Vilma Jacobo, Roberto Zimmer, Patricia Andrea Elena, Sebastián Alejandro Nicola, Ana M. Torioni, Susana Marta Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas |
| title | Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas |
| title_full | Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas |
| title_fullStr | Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas |
| title_full_unstemmed | Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas |
| title_short | Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas |
| title_sort | pheno and genotyping of brucella abortus biovar 5 isolated from a water buffalo bubalus bubalis fetus first case reported in the americas |
| topic | Búfalo de Agua Brucella Abortus Genotipos Fenotipos Brucelosis Water Buffaloes Genotypes Phenotypes Brucellosis América Latina |
| url | http://hdl.handle.net/20.500.12123/2688 https://www.sciencedirect.com/science/article/pii/S0378113514003344?via%3Dihub https://doi.org/10.1016/j.vetmic.2014.07.011 |
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