Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
Allium sativum is an important crop species in Argentina, one of the major garlic exporting countries in the world. Because garlic is an agamic propagated species, its breeding is based on the occurrence of plants with desirable traits, which are attributed to genetic mutations. In this work, we too...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0304423818302930 http://hdl.handle.net/20.500.12123/2660 https://doi.org/10.1016/j.scienta.2018.04.044 |
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| author | Gimenez, Magali Diana Garcia Lampasona, Sandra Claudia |
| author_browse | Garcia Lampasona, Sandra Claudia Gimenez, Magali Diana |
| author_facet | Gimenez, Magali Diana Garcia Lampasona, Sandra Claudia |
| author_sort | Gimenez, Magali Diana |
| collection | INTA Digital |
| description | Allium sativum is an important crop species in Argentina, one of the major garlic exporting countries in the world. Because garlic is an agamic propagated species, its breeding is based on the occurrence of plants with desirable traits, which are attributed to genetic mutations. In this work, we took advantage of a long term field experiment in a garlic line destined to genetic improvement to evaluate bulb weight and genetic and epigenetic stability and dynamics over 13 years. Average bulb weight increased from 65.3 g in the first year to 84.8 g the 13th year. Using AFLP (Amplified Fragment Length Polymorphism) and MSAP (Methylation Sensitive Amplified Polymorphism) techniques, we assessed genetic and epigenetic stability in plants of A. sativum taken at first, third and 13th year of field culture. We detected that 82.47 and 19.61% of the AFLP and MSAP fragments were present in the tree years of sampling. The application of selection pressure led to a reduction in genetic polymorphism and an increase of bulb weight, but did not influence epigenetic polymorphism, indicating that it is independent of genetic variability. We detected changes in the amount of each methylation pattern (unmethylated, hemimethylated and internally methylated) among the different years analyzed while 82.47 and 19.61% of the genetic and epigenetical loci were stable during the time of culture. Although some epiloci showed stability along the 13 years of culture, others presented gradual variation, while others were polymorphic within samples from the same year of culture. Finally, we discussed the implications of the high epigenetic variability of an agamic propagated species and its possible effect on the phenotype. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2660 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26602018-06-19T14:28:19Z Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment Gimenez, Magali Diana Garcia Lampasona, Sandra Claudia Allium Sativum Ajo Marcadores Genéticos Epigenético Estabilidad Genética Garlic Genetic Markers Epigenetics Genetic Stability Argentina Allium sativum is an important crop species in Argentina, one of the major garlic exporting countries in the world. Because garlic is an agamic propagated species, its breeding is based on the occurrence of plants with desirable traits, which are attributed to genetic mutations. In this work, we took advantage of a long term field experiment in a garlic line destined to genetic improvement to evaluate bulb weight and genetic and epigenetic stability and dynamics over 13 years. Average bulb weight increased from 65.3 g in the first year to 84.8 g the 13th year. Using AFLP (Amplified Fragment Length Polymorphism) and MSAP (Methylation Sensitive Amplified Polymorphism) techniques, we assessed genetic and epigenetic stability in plants of A. sativum taken at first, third and 13th year of field culture. We detected that 82.47 and 19.61% of the AFLP and MSAP fragments were present in the tree years of sampling. The application of selection pressure led to a reduction in genetic polymorphism and an increase of bulb weight, but did not influence epigenetic polymorphism, indicating that it is independent of genetic variability. We detected changes in the amount of each methylation pattern (unmethylated, hemimethylated and internally methylated) among the different years analyzed while 82.47 and 19.61% of the genetic and epigenetical loci were stable during the time of culture. Although some epiloci showed stability along the 13 years of culture, others presented gradual variation, while others were polymorphic within samples from the same year of culture. Finally, we discussed the implications of the high epigenetic variability of an agamic propagated species and its possible effect on the phenotype. EEA Mendoza Fil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina 2018-06-19T14:26:50Z 2018-06-19T14:26:50Z 2018-10-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0304423818302930 http://hdl.handle.net/20.500.12123/2660 0304-4238 https://doi.org/10.1016/j.scienta.2018.04.044 eng info:eu-repo/semantics/restrictedAccess application/pdf Scientia Horticulturae 240 : 23-28 (October 2018) |
| spellingShingle | Allium Sativum Ajo Marcadores Genéticos Epigenético Estabilidad Genética Garlic Genetic Markers Epigenetics Genetic Stability Argentina Gimenez, Magali Diana Garcia Lampasona, Sandra Claudia Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment |
| title | Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment |
| title_full | Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment |
| title_fullStr | Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment |
| title_full_unstemmed | Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment |
| title_short | Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment |
| title_sort | before after analysis of genetic and epigenetic markers in garlic a 13 year experiment |
| topic | Allium Sativum Ajo Marcadores Genéticos Epigenético Estabilidad Genética Garlic Genetic Markers Epigenetics Genetic Stability Argentina |
| url | https://www.sciencedirect.com/science/article/pii/S0304423818302930 http://hdl.handle.net/20.500.12123/2660 https://doi.org/10.1016/j.scienta.2018.04.044 |
| work_keys_str_mv | AT gimenezmagalidiana beforeafteranalysisofgeneticandepigeneticmarkersingarlica13yearexperiment AT garcialampasonasandraclaudia beforeafteranalysisofgeneticandepigeneticmarkersingarlica13yearexperiment |