Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden
In tissue culture, essential aspects of growth and development can be modulated by managing mineral nutrition and growth regulators. Therefore, basal medium selection is one of the key factors for success with this technique since the nutritional status of tissue cultures relies on it. Although Mura...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://link.springer.com/article/10.1007/s11627-015-9740-7 http://hdl.handle.net/20.500.12123/2624 https://doi.org/10.1007/s11627-015-9740-7 |
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| author | Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal |
| author_browse | Gonçalves, Antônio Natal Oberschelp, Gustavo Pedro Javier |
| author_facet | Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal |
| author_sort | Oberschelp, Gustavo Pedro Javier |
| collection | INTA Digital |
| description | In tissue culture, essential aspects of growth and development can be modulated by managing mineral nutrition and growth regulators. Therefore, basal medium selection is one of the key factors for success with this technique since the nutritional status of tissue cultures relies on it. Although Murashige and Skoog (MS), JADS, and woody plant medium (WPM) media are among the most common basal media used for the in vitro propagation of eucalypts, they have shown limited success for use in Eucalyptus dunnii micropropagation, where excessive callus formation, hyperhydricity, and chlorosis are frequent issues. These morpho-physiological responses could be related to different nutritional conditions provided by each media, showing that none of them was competent for E. dunnii micropropagation. To prevent these problems, a modified version of a novel basal medium for E. dunnii (EDMm), based on chemical analysis of E. dunnii young stump shoots was developed. The morpho-physiological responses and mineral nutrient contents of four E. dunnii clones cultured in EDMm, JADS, MS, and WPM basal media were assessed. Multivariate analysis suggested that Cu, Fe, Mn, and Zn concentrations in tissues, all of which are at higher concentrations in EDMm medium than the other media, were associated to higher rooting rates. On the other hand, higher K and S concentrations were related to higher multiplication and growth rates. The results showed that all basal media tested support axillary shoot multiplication, but only the shoots grown in EDMm media presented no Fe chlorosis or oxidation symptoms and showed higher rooting rates. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2624 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26242019-09-11T18:30:32Z Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal Eucalyptus Regeneración in Vitro Oligoelementos Nutrición de las Plantas In Vitro Regeneration Trace Elements Plant Nutrition Eucalyptus dunnii Propagación in vitro Micronutrientes In tissue culture, essential aspects of growth and development can be modulated by managing mineral nutrition and growth regulators. Therefore, basal medium selection is one of the key factors for success with this technique since the nutritional status of tissue cultures relies on it. Although Murashige and Skoog (MS), JADS, and woody plant medium (WPM) media are among the most common basal media used for the in vitro propagation of eucalypts, they have shown limited success for use in Eucalyptus dunnii micropropagation, where excessive callus formation, hyperhydricity, and chlorosis are frequent issues. These morpho-physiological responses could be related to different nutritional conditions provided by each media, showing that none of them was competent for E. dunnii micropropagation. To prevent these problems, a modified version of a novel basal medium for E. dunnii (EDMm), based on chemical analysis of E. dunnii young stump shoots was developed. The morpho-physiological responses and mineral nutrient contents of four E. dunnii clones cultured in EDMm, JADS, MS, and WPM basal media were assessed. Multivariate analysis suggested that Cu, Fe, Mn, and Zn concentrations in tissues, all of which are at higher concentrations in EDMm medium than the other media, were associated to higher rooting rates. On the other hand, higher K and S concentrations were related to higher multiplication and growth rates. The results showed that all basal media tested support axillary shoot multiplication, but only the shoots grown in EDMm media presented no Fe chlorosis or oxidation symptoms and showed higher rooting rates. EEA Concordia Fil: Oberschelp, Gustavo Pedro Javier. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Gonçalves, Antônio Natal. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil 2018-06-14T13:54:55Z 2018-06-14T13:54:55Z 2016-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s11627-015-9740-7 http://hdl.handle.net/20.500.12123/2624 1054-5476 1475-2689 https://doi.org/10.1007/s11627-015-9740-7 eng info:eu-repo/semantics/restrictedAccess application/pdf In vitro cellular & developmental bology - Plant 52 (1) : 28–37. (February 2016) |
| spellingShingle | Eucalyptus Regeneración in Vitro Oligoelementos Nutrición de las Plantas In Vitro Regeneration Trace Elements Plant Nutrition Eucalyptus dunnii Propagación in vitro Micronutrientes Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden |
| title | Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden |
| title_full | Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden |
| title_fullStr | Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden |
| title_full_unstemmed | Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden |
| title_short | Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden |
| title_sort | assessing the effects of basal media on the in vitro propagation and nutritional status of eucalyptus dunnii maiden |
| topic | Eucalyptus Regeneración in Vitro Oligoelementos Nutrición de las Plantas In Vitro Regeneration Trace Elements Plant Nutrition Eucalyptus dunnii Propagación in vitro Micronutrientes |
| url | https://link.springer.com/article/10.1007/s11627-015-9740-7 http://hdl.handle.net/20.500.12123/2624 https://doi.org/10.1007/s11627-015-9740-7 |
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