Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots
Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competen...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://link.springer.com/article/10.1007/s11627-015-9715-8 http://hdl.handle.net/20.500.12123/2623 https://doi.org/10.1007/s11627-015-9715-8 |
| _version_ | 1855483089047781376 |
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| author | Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal Meneghetti, Eveline Calderan Graner, Érika Mendes Almeida, Marcílio de |
| author_browse | Almeida, Marcílio de Gonçalves, Antônio Natal Graner, Érika Mendes Meneghetti, Eveline Calderan Oberschelp, Gustavo Pedro Javier |
| author_facet | Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal Meneghetti, Eveline Calderan Graner, Érika Mendes Almeida, Marcílio de |
| author_sort | Oberschelp, Gustavo Pedro Javier |
| collection | INTA Digital |
| description | Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators. |
| format | Artículo |
| id | INTA2623 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26232019-06-12T12:18:43Z Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal Meneghetti, Eveline Calderan Graner, Érika Mendes Almeida, Marcílio de Eucalyptus Regeneración Regeneración in Vitro Enraizamiento Organogénesis Regeneration In Vitro Regeneration Rooting Organogenesis Eucalyptus dunnii Propagación in vitro Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combinations of 6-benzylaminopurine (BAP) plus α-naphthalene acetic acid (NAA) or indole-3-acetic acid (IAA) were added (0, 0.1, 0.5, and 1 mg l−1) to a specifically developed basal medium. Moreover, the effect of the hypocotyl segments position (distal and proximal) on bud and callus regeneration was studied. Finally, microcuttings obtained from in vitro multiplication of the regenerated buds were treated with different dosages of indole-3-butyric acid (IBA) or NAA (powder based) and rooted under ex vitro conditions. Bud regeneration was observed only in hypocotyl segments, where dosages of 0.5 mg l−1 BAP plus 0.5 mg l−1 NAA or 0.5 mg l−1 IAA and distal segments resulted in higher bud regeneration rates. Histological analyses suggest that callus regeneration areas in hypocotyl explants recreate an appropriate niche for the development of pluripotent cells that give rise to buds and shoots. Either IBA or NAA treatments failed to significantly improved the ex vitro rooting of E. dunnii microcuttings, whereas >50% microcuttings rooted without application of growth regulators. EEA Concordia Fil: Oberschelp, Gustavo Pedro Javier. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Gonçalves, Antônio Natal. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil Fil: Meneghetti, Eveline Calderan. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil Fil: Graner, Érika Mendes. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil Fil: Almeida, Marcílio de. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”, Depto. de Ciências Florestais, PPG em Recursos Florestais; Brasil. Universidade de São Paulo. Escola Superior de Agricultura “Luiz de Queiroz”. Depto. de Ciências Biológicas. PPG em Fisiologia e Bioquímica de Plantas; Brasil 2018-06-14T13:34:47Z 2018-06-14T13:34:47Z 2015-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s11627-015-9715-8 http://hdl.handle.net/20.500.12123/2623 1054-5476 1475-2689 https://doi.org/10.1007/s11627-015-9715-8 eng info:eu-repo/semantics/restrictedAccess application/pdf In vitro cellular & developmental biology - Plant 51 (6) : 626–636. (December 2015) |
| spellingShingle | Eucalyptus Regeneración Regeneración in Vitro Enraizamiento Organogénesis Regeneration In Vitro Regeneration Rooting Organogenesis Eucalyptus dunnii Propagación in vitro Oberschelp, Gustavo Pedro Javier Gonçalves, Antônio Natal Meneghetti, Eveline Calderan Graner, Érika Mendes Almeida, Marcílio de Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots |
| title | Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots |
| title_full | Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots |
| title_fullStr | Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots |
| title_full_unstemmed | Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots |
| title_short | Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots |
| title_sort | eucalyptus dunnii maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots |
| topic | Eucalyptus Regeneración Regeneración in Vitro Enraizamiento Organogénesis Regeneration In Vitro Regeneration Rooting Organogenesis Eucalyptus dunnii Propagación in vitro |
| url | https://link.springer.com/article/10.1007/s11627-015-9715-8 http://hdl.handle.net/20.500.12123/2623 https://doi.org/10.1007/s11627-015-9715-8 |
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