Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes
Twelve alfalfa genotypes that were selected for biomass under salinity, differences in Na and Cl concentrations in shoots and K/Na ratio were evaluated in this long-term salinity experiment. The selected plants were cloned to reduce genetic variability within each genotype. Salt tolerance (ST) index...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.nature.com/articles/srep42958 http://hdl.handle.net/20.500.12123/2611 https://doi.org/10.1038/srep42958 |
| _version_ | 1855483086729379840 |
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| author | Sandhu, Devinder Cornacchione, Monica Ferreira, Jorge F.S. Suarez, Donald L. |
| author_browse | Cornacchione, Monica Ferreira, Jorge F.S. Sandhu, Devinder Suarez, Donald L. |
| author_facet | Sandhu, Devinder Cornacchione, Monica Ferreira, Jorge F.S. Suarez, Donald L. |
| author_sort | Sandhu, Devinder |
| collection | INTA Digital |
| description | Twelve alfalfa genotypes that were selected for biomass under salinity, differences in Na and Cl concentrations in shoots and K/Na ratio were evaluated in this long-term salinity experiment. The selected plants were cloned to reduce genetic variability within each genotype. Salt tolerance (ST) index of the genotypes ranged from 0.39 to 1. The most salt-tolerant genotypes SISA14-1 (G03) and AZ-90ST (G10), the top performers for biomass, exhibited the least effect on shoot number and height. SISA14-1 (G03) accumulated low Na and Cl under salinity. Most genotypes exhibited a net reduction in shoot Ca, Mg, P, Fe, and Cu, while Mn and Zn increased under salinity. Salinity reduced foliar area and stomatal conductance; while net photosynthetic rate and transpiration were not affected. Interestingly, salinity increased chlorophyll and antioxidant capacity in most genotypes; however neither parameter correlated well to ST index. Salt-tolerant genotypes showed upregulation of the SOS1, SOS2, SOS3, HKT1, AKT1, NHX1, P5CS1, HSP90.7, HSP81.2, HSP71.1, HSPC025, OTS1, SGF29 and SAL1 genes. Gene expression analyses allowed us to classify genotypes based on their ability to regulate different components of the salt tolerance mechanism. Pyramiding different components of the salt tolerance mechanism may lead to superior salt-tolerant alfalfa genotypes. |
| format | Artículo |
| id | INTA2611 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26112018-06-13T13:28:33Z Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes Sandhu, Devinder Cornacchione, Monica Ferreira, Jorge F.S. Suarez, Donald L. Medicago Sativa Genotipos Genes Salinidad Variación Genética Genotypes Salinity Genetic Variation Alfalfa Twelve alfalfa genotypes that were selected for biomass under salinity, differences in Na and Cl concentrations in shoots and K/Na ratio were evaluated in this long-term salinity experiment. The selected plants were cloned to reduce genetic variability within each genotype. Salt tolerance (ST) index of the genotypes ranged from 0.39 to 1. The most salt-tolerant genotypes SISA14-1 (G03) and AZ-90ST (G10), the top performers for biomass, exhibited the least effect on shoot number and height. SISA14-1 (G03) accumulated low Na and Cl under salinity. Most genotypes exhibited a net reduction in shoot Ca, Mg, P, Fe, and Cu, while Mn and Zn increased under salinity. Salinity reduced foliar area and stomatal conductance; while net photosynthetic rate and transpiration were not affected. Interestingly, salinity increased chlorophyll and antioxidant capacity in most genotypes; however neither parameter correlated well to ST index. Salt-tolerant genotypes showed upregulation of the SOS1, SOS2, SOS3, HKT1, AKT1, NHX1, P5CS1, HSP90.7, HSP81.2, HSP71.1, HSPC025, OTS1, SGF29 and SAL1 genes. Gene expression analyses allowed us to classify genotypes based on their ability to regulate different components of the salt tolerance mechanism. Pyramiding different components of the salt tolerance mechanism may lead to superior salt-tolerant alfalfa genotypes. EEA Santiago del Estero Fil: Sandhu, Devinder. USDA. Agricultural Research Service. US Salinity Lab; Estados Unidos Fil: Cornacchione, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina Fil: Ferreira, Jorge F.S. USDA. Agricultural Research Service. US Salinity Lab; Estados Unidos Fil: Suarez, Donald L. USDA. Agricultural Research Service. US Salinity Lab; Estados Unidos 2018-06-13T13:27:18Z 2018-06-13T13:27:18Z 2017-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.nature.com/articles/srep42958 http://hdl.handle.net/20.500.12123/2611 2045-2322 https://doi.org/10.1038/srep42958 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Scientific Reports 7 : Article number: 42958 (2017) |
| spellingShingle | Medicago Sativa Genotipos Genes Salinidad Variación Genética Genotypes Salinity Genetic Variation Alfalfa Sandhu, Devinder Cornacchione, Monica Ferreira, Jorge F.S. Suarez, Donald L. Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes |
| title | Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes |
| title_full | Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes |
| title_fullStr | Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes |
| title_full_unstemmed | Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes |
| title_short | Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes |
| title_sort | variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt response genes |
| topic | Medicago Sativa Genotipos Genes Salinidad Variación Genética Genotypes Salinity Genetic Variation Alfalfa |
| url | https://www.nature.com/articles/srep42958 http://hdl.handle.net/20.500.12123/2611 https://doi.org/10.1038/srep42958 |
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