Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
Peaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12665 http://hdl.handle.net/20.500.12123/2604 https://doi.org/10.1111/ppl.12665 |
| _version_ | 1855483085381959680 |
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| author | Bustamante, Claudia Anabel Brotman, Yavid Monti, Laura L. Gabilondo, Julieta Budde, Claudio Olaf Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana |
| author_browse | Brotman, Yavid Budde, Claudio Olaf Bustamante, Claudia Anabel Drincovich, María Fabiana Fernie, Alisdair R. Gabilondo, Julieta Lara, María Valeria Monti, Laura L. |
| author_facet | Bustamante, Claudia Anabel Brotman, Yavid Monti, Laura L. Gabilondo, Julieta Budde, Claudio Olaf Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana |
| author_sort | Bustamante, Claudia Anabel |
| collection | INTA Digital |
| description | Peaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects of chilling, the lipidome of six peach varieties with different susceptibility to CI was analyzed under different postharvest conditions. By using liquid chromatography coupled to mass spectrometry (LC–MS), 59 lipid species were detected, including diacyl- and triacylglycerides. The decreases in fruit firmness during postharvest ripening were accompanied by changes in the relative amount of several plastidic glycerolipid and triacylglyceride species, which may indicate their use as fuels prior to fruit senescence. In addition, levels of galactolipids were also
modified in fruits stored at 0∘C for short and long periods, reflecting the stabilization of plastidic membranes at low temperature. When comparing susceptible and resistant varieties, the relative abundance of certain species of the lipid classes phosphatidylethanolamine, phosphatidylcholine and digalactosyldiacylglycerol correlated with the tolerance to CI, reflecting the importance of the plasma membrane in the development of CI symptoms and allowing the identification of possible lipid markers for chilling resistance. Finally, transcriptional analysis of genes involved in galactolipid metabolism revealed
candidate genes responsible for the observed changes after cold exposure. When taken together, our results highlight the importance of plastids in the postharvest physiology of fruits and provide evidence that lipid composition and metabolism have a profound influence on the cold response. |
| format | Artículo |
| id | INTA2604 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26042019-06-06T17:11:01Z Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury Bustamante, Claudia Anabel Brotman, Yavid Monti, Laura L. Gabilondo, Julieta Budde, Claudio Olaf Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana Durazno Prunus persica Almacenamiento en Frío Fisiologia Postcosecha Metabolismo de Lípidos Daño por Frío Variedades Maduración en Postcosecha Peaches Cold Storage Postharvest Physiology Lipid Metabolism Chilling Injury Varieties Postharvest Ripening Peaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects of chilling, the lipidome of six peach varieties with different susceptibility to CI was analyzed under different postharvest conditions. By using liquid chromatography coupled to mass spectrometry (LC–MS), 59 lipid species were detected, including diacyl- and triacylglycerides. The decreases in fruit firmness during postharvest ripening were accompanied by changes in the relative amount of several plastidic glycerolipid and triacylglyceride species, which may indicate their use as fuels prior to fruit senescence. In addition, levels of galactolipids were also modified in fruits stored at 0∘C for short and long periods, reflecting the stabilization of plastidic membranes at low temperature. When comparing susceptible and resistant varieties, the relative abundance of certain species of the lipid classes phosphatidylethanolamine, phosphatidylcholine and digalactosyldiacylglycerol correlated with the tolerance to CI, reflecting the importance of the plasma membrane in the development of CI symptoms and allowing the identification of possible lipid markers for chilling resistance. Finally, transcriptional analysis of genes involved in galactolipid metabolism revealed candidate genes responsible for the observed changes after cold exposure. When taken together, our results highlight the importance of plastids in the postharvest physiology of fruits and provide evidence that lipid composition and metabolism have a profound influence on the cold response. EEA San Pedro Fil: Bustamante, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Brotman, Yariv. Ben Gurion University of the Negev. Department of Life Sciences; Israel Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Lara, Maria V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania Fil: Drincovich, Maria Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina 2018-06-12T15:31:06Z 2018-06-12T15:31:06Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12665 http://hdl.handle.net/20.500.12123/2604 1399-3054 0031-9317 https://doi.org/10.1111/ppl.12665 eng info:eu-repograntAgreement/INTA/PNFRU/1105083/AR./Nuevas tecnologías para el mantenimiento de la calidad en la cosecha, acondicionamiento y logística de frutas frescas. info:eu-repo/semantics/restrictedAccess application/pdf Physiologia plantarum, vol. 163. 2018. p. 2-17 |
| spellingShingle | Durazno Prunus persica Almacenamiento en Frío Fisiologia Postcosecha Metabolismo de Lípidos Daño por Frío Variedades Maduración en Postcosecha Peaches Cold Storage Postharvest Physiology Lipid Metabolism Chilling Injury Varieties Postharvest Ripening Bustamante, Claudia Anabel Brotman, Yavid Monti, Laura L. Gabilondo, Julieta Budde, Claudio Olaf Lara, María Valeria Fernie, Alisdair R. Drincovich, María Fabiana Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury |
| title | Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury |
| title_full | Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury |
| title_fullStr | Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury |
| title_full_unstemmed | Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury |
| title_short | Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury |
| title_sort | differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury |
| topic | Durazno Prunus persica Almacenamiento en Frío Fisiologia Postcosecha Metabolismo de Lípidos Daño por Frío Variedades Maduración en Postcosecha Peaches Cold Storage Postharvest Physiology Lipid Metabolism Chilling Injury Varieties Postharvest Ripening |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12665 http://hdl.handle.net/20.500.12123/2604 https://doi.org/10.1111/ppl.12665 |
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