Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle
Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four cop...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.hindawi.com/journals/jir/2018/3497401/ http://hdl.handle.net/20.500.12123/2601 https://doi.org/10.1155/2018/3497401 |
| _version_ | 1855483085001326592 |
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| author | Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Perez Filgueira, Daniel Mariano Pega, Juan Franco Gnazzo, Victoria Romera, Sonia Alejandra Schammas, Juan Manuel Bucafusco, Danilo Di Giacomo, Sebastián García de la Torre, Beatriz Andreu, David Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines |
| author_browse | Andreu, David Blanco, Esther Bucafusco, Danilo Di Giacomo, Sebastián García de la Torre, Beatriz Gnazzo, Victoria Langellotti, Cecilia Ana Pega, Juan Franco Perez Filgueira, Daniel Mariano Quattrocchi, Valeria Romera, Sonia Alejandra Schammas, Juan Manuel Sobrino, Francisco Soria, Ivana Zamorano, Patricia Ines |
| author_facet | Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Perez Filgueira, Daniel Mariano Pega, Juan Franco Gnazzo, Victoria Romera, Sonia Alejandra Schammas, Juan Manuel Bucafusco, Danilo Di Giacomo, Sebastián García de la Torre, Beatriz Andreu, David Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines |
| author_sort | Soria, Ivana |
| collection | INTA Digital |
| description | Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four copies of a peptide corresponding to the B-cell epitope VP1 [136–154] of type O FMDV (O/UKG/11/2001) linked through thioether bonds to a single copy of the T-cell epitope 3A [21–35] (termed B2T and B4T, resp.) afforded protection in vaccinated pigs. In this work, we show that dendrimeric peptides B2T and B4T can elicit specific humoral responses in cattle and confer partial protection against the challenge with a heterologous type O virus (O1/Campos/Bra/58). This protective response correlated with the induction of specific T-cells as well as with an anamnestic antibody response upon virus challenge, as shown by the detection of virus-specific antibody-secreting cells (ASC) in lymphoid tissues distal from the inoculation point. |
| format | Artículo |
| id | INTA2601 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26012018-06-12T14:17:35Z Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Perez Filgueira, Daniel Mariano Pega, Juan Franco Gnazzo, Victoria Romera, Sonia Alejandra Schammas, Juan Manuel Bucafusco, Danilo Di Giacomo, Sebastián García de la Torre, Beatriz Andreu, David Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Péptidos Respuesta Inmunológica Cattle Animal Diseases Foot and Mouth Disease Peptides Immune Response Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to induce solid protection in cattle. Dendrimeric peptides displaying two or four copies of a peptide corresponding to the B-cell epitope VP1 [136–154] of type O FMDV (O/UKG/11/2001) linked through thioether bonds to a single copy of the T-cell epitope 3A [21–35] (termed B2T and B4T, resp.) afforded protection in vaccinated pigs. In this work, we show that dendrimeric peptides B2T and B4T can elicit specific humoral responses in cattle and confer partial protection against the challenge with a heterologous type O virus (O1/Campos/Bra/58). This protective response correlated with the induction of specific T-cells as well as with an anamnestic antibody response upon virus challenge, as shown by the detection of virus-specific antibody-secreting cells (ASC) in lymphoid tissues distal from the inoculation point. Instituto de Virología Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gnazzo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Garcia de la Torre, Beatriz. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España Fil: Andreu, David. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España Fil: Sobrino, Francisco. Universidad Autónoma de Madrid. Centro de Biología Molecular “Severo Ochoa"; España Fil: Blanco, Esther. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación en Sanidad Animal; España Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina 2018-06-12T13:46:22Z 2018-06-12T13:46:22Z 2018-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.hindawi.com/journals/jir/2018/3497401/ http://hdl.handle.net/20.500.12123/2601 2314-8861 2314-7156 https://doi.org/10.1155/2018/3497401 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Immunology Research 2018 : Article ID 3497401, 12 pages (April 2018) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Péptidos Respuesta Inmunológica Cattle Animal Diseases Foot and Mouth Disease Peptides Immune Response Soria, Ivana Quattrocchi, Valeria Langellotti, Cecilia Ana Perez Filgueira, Daniel Mariano Pega, Juan Franco Gnazzo, Victoria Romera, Sonia Alejandra Schammas, Juan Manuel Bucafusco, Danilo Di Giacomo, Sebastián García de la Torre, Beatriz Andreu, David Sobrino, Francisco Blanco, Esther Zamorano, Patricia Ines Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_full | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_fullStr | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_full_unstemmed | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_short | Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle |
| title_sort | immune response and partial protection against heterologous foot and mouth disease virus induced by dendrimer peptides in cattle |
| topic | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Péptidos Respuesta Inmunológica Cattle Animal Diseases Foot and Mouth Disease Peptides Immune Response |
| url | https://www.hindawi.com/journals/jir/2018/3497401/ http://hdl.handle.net/20.500.12123/2601 https://doi.org/10.1155/2018/3497401 |
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