Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions
Numerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic nitrogen to the soil, and their increased nutritional value. Alfalfa is the main so...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs00122-018-3062-1 http://hdl.handle.net/20.500.12123/2599 https://doi.org/10.1007/s00122-018-3062-1 |
| _version_ | 1855034991456550912 |
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| author | Jozefkowicz, Cintia Frare, Romina Alejandra Odorizzi, Ariel Arolfo, Valeria Pagano, Elba Maria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cinthia |
| author_browse | Arolfo, Valeria Ayub, Nicolás Daniel Basigalup, Daniel Horacio Frare, Romina Alejandra Jozefkowicz, Cintia Odorizzi, Ariel Pagano, Elba Maria Soto, Gabriela Cinthia |
| author_facet | Jozefkowicz, Cintia Frare, Romina Alejandra Odorizzi, Ariel Arolfo, Valeria Pagano, Elba Maria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cinthia |
| author_sort | Jozefkowicz, Cintia |
| collection | INTA Digital |
| description | Numerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic nitrogen to the soil, and their increased nutritional value. Alfalfa is the main source of vegetable proteins in meat and milk production systems worldwide. Despite the economic and ecological importance of this autotetraploid and allogamous forage crop, little progress has been made in the incorporation of transgenic traits into commercial alfalfa. This is mainly due to the unusually strong transgene silencing and complex reproductive behavior of alfalfa, which limit the production of events with high transgene expression and the introgression of selected events within heterogeneous synthetic populations, respectively. In this report, we describe a novel procedure, called supertransgene process, where a glufosinate-tolerant alfalfa variety was developed using a single event containing the BAR transgene associated with an inversion. This approach can be used to maximize the expression of transgenic traits into elite alfalfa germplasm and to reduce the cost of production of transgenic alfalfa cultivars, contributing to the public improvement of this legume forage and other polyploid and outcrossing crop species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2599 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA25992019-06-12T12:11:07Z Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions Jozefkowicz, Cintia Frare, Romina Alejandra Odorizzi, Ariel Arolfo, Valeria Pagano, Elba Maria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cinthia Medicago Sativa Germoplasma Transgénicos Variedades Germplasm Transgenics Varieties Alfalfa Numerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic nitrogen to the soil, and their increased nutritional value. Alfalfa is the main source of vegetable proteins in meat and milk production systems worldwide. Despite the economic and ecological importance of this autotetraploid and allogamous forage crop, little progress has been made in the incorporation of transgenic traits into commercial alfalfa. This is mainly due to the unusually strong transgene silencing and complex reproductive behavior of alfalfa, which limit the production of events with high transgene expression and the introgression of selected events within heterogeneous synthetic populations, respectively. In this report, we describe a novel procedure, called supertransgene process, where a glufosinate-tolerant alfalfa variety was developed using a single event containing the BAR transgene associated with an inversion. This approach can be used to maximize the expression of transgenic traits into elite alfalfa germplasm and to reduce the cost of production of transgenic alfalfa cultivars, contributing to the public improvement of this legume forage and other polyploid and outcrossing crop species. Instituto de Genética Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Frare, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Odorizzi, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Arolfo, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Basigalup, Daniel Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2018-06-12T12:46:57Z 2018-06-12T12:46:57Z 2018-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs00122-018-3062-1 http://hdl.handle.net/20.500.12123/2599 0040-5752 1432-2242 https://doi.org/10.1007/s00122-018-3062-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Theoretical and applied genetics 131 (5) : 1111–1123. (May 2018) |
| spellingShingle | Medicago Sativa Germoplasma Transgénicos Variedades Germplasm Transgenics Varieties Alfalfa Jozefkowicz, Cintia Frare, Romina Alejandra Odorizzi, Ariel Arolfo, Valeria Pagano, Elba Maria Basigalup, Daniel Horacio Ayub, Nicolás Daniel Soto, Gabriela Cinthia Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions |
| title | Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions |
| title_full | Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions |
| title_fullStr | Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions |
| title_full_unstemmed | Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions |
| title_short | Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions |
| title_sort | maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions |
| topic | Medicago Sativa Germoplasma Transgénicos Variedades Germplasm Transgenics Varieties Alfalfa |
| url | https://link.springer.com/article/10.1007%2Fs00122-018-3062-1 http://hdl.handle.net/20.500.12123/2599 https://doi.org/10.1007/s00122-018-3062-1 |
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