A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2596 https://link.springer.com/article/10.1007/s10658-015-0762-0 https://doi.org/10.1007/s10658-015-0762-0 |
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| author | Albarracín Orio, Andrea Georgina Brücher, Elsa Ducasse, Daniel Adrian |
| author_browse | Albarracín Orio, Andrea Georgina Brücher, Elsa Ducasse, Daniel Adrian |
| author_facet | Albarracín Orio, Andrea Georgina Brücher, Elsa Ducasse, Daniel Adrian |
| author_sort | Albarracín Orio, Andrea Georgina |
| collection | INTA Digital |
| description | Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina.We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiledfungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible
bacterial inhibitory compounds specifically induced by this pathogen. |
| format | Artículo |
| id | INTA2596 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA25962019-06-12T12:10:01Z A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris Albarracín Orio, Andrea Georgina Brücher, Elsa Ducasse, Daniel Adrian Bacillus Subtilis Control Biológico Cebolla Allium Cepa Organismos Transmitidos por Suelo Soilborne Organisms Onions Biological Control Setophoma Terrestris Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the strategies currently used are ineffective. Although, there are some varieties resistant to S. terrestris, they are not resistant to every isolate of the pathogen and it is not unusual to find isolates that break that resistance. Moreover, chemical control is not technically or economically feasible. So, we decided to address biocontrol as a mean to manage the main onion disease in Argentina.We tested the efficiency of a strain of Bacillus subtilis subsp. subtilis isolated from the rhizosphere of onion plants to inhibit the growth of S. terrestris in vitro. Our strain of Bacillus subtilis showed a strong capacity of growth inhibition of S. terrestris. No antagonistic activity against two other onion pathogens, Fusarium oxysporum f. sp. cepae and F. proliferatum, was observed. Interestingly, we found a high growth inhibition of S. terrestris on plates containing cell-free supernatant of B. subtilis previously grown in the presence of the fungus. No significant differences in the fungal growth were obtained between control plates and plates containing cell-free supernatant from B. subtilis grown without previous contact with S. terrestris. Electron microscopy of S. terrestris from co-cultures plates revealed thickened, tortuous or coiledfungal hyphae, with granules and globular like terminations. These results suggested that the strain of B. subtilis under study has a strong biocontrol activity against S. terrestris and that it would be acting diffusible bacterial inhibitory compounds specifically induced by this pathogen. Instituto de Patología Vegetal Fil: Albarracín Orio, Andrea Georgina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina Fil: Brücher, Elsa. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina 2018-06-11T18:29:50Z 2018-06-11T18:29:50Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2596 https://link.springer.com/article/10.1007/s10658-015-0762-0 0929-1873 1573-8469 (Online) https://doi.org/10.1007/s10658-015-0762-0 eng info:eu-repo/semantics/restrictedAccess application/pdf European journal of plant pathology 144 (1) : 217–223. (January 2016) |
| spellingShingle | Bacillus Subtilis Control Biológico Cebolla Allium Cepa Organismos Transmitidos por Suelo Soilborne Organisms Onions Biological Control Setophoma Terrestris Albarracín Orio, Andrea Georgina Brücher, Elsa Ducasse, Daniel Adrian A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris |
| title | A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris |
| title_full | A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris |
| title_fullStr | A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris |
| title_full_unstemmed | A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris |
| title_short | A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris |
| title_sort | strain of bacillus subtilis subsp subtilis shows a specific antagonistic activity against the soil borne pathogen of onion setophoma terrestris |
| topic | Bacillus Subtilis Control Biológico Cebolla Allium Cepa Organismos Transmitidos por Suelo Soilborne Organisms Onions Biological Control Setophoma Terrestris |
| url | http://hdl.handle.net/20.500.12123/2596 https://link.springer.com/article/10.1007/s10658-015-0762-0 https://doi.org/10.1007/s10658-015-0762-0 |
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