Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection
Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local lymphoid tissues alon...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://jvi.asm.org/content/89/18/9581.abstract http://hdl.handle.net/20.500.12123/2536 http://dx.doi.org/10.1128/JVI.01082-15 |
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| author | Pega, Juan Franco Di Giacomo, Sebastián Bucafusco, Danilo Schammas, Juan Manuel Malacari, Darío Amilcar Barrionuevo, Florencia Mariel Capozzo, Alejandra Victoria Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano |
| author_browse | Barrionuevo, Florencia Mariel Borca, Manuel Victor Bucafusco, Danilo Capozzo, Alejandra Victoria Di Giacomo, Sebastián Malacari, Darío Amilcar Pega, Juan Franco Perez Filgueira, Daniel Mariano Rodríguez, L.L. Schammas, Juan Manuel |
| author_facet | Pega, Juan Franco Di Giacomo, Sebastián Bucafusco, Danilo Schammas, Juan Manuel Malacari, Darío Amilcar Barrionuevo, Florencia Mariel Capozzo, Alejandra Victoria Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano |
| author_sort | Pega, Juan Franco |
| collection | INTA Digital |
| description | Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local lymphoid tissues along the respiratory system in vaccinated and further aerosol-infected cattle. Animals immunized with a high-payload monovalent FMD vaccine developed high titers of neutralizing antibodies at 7 days postvaccination (dpv), reaching a plateau at 29 dpv. FMDV-specific antibody-secreting cells (ASC), predominantly IgM, were evident at 7 dpv in the prescapular lymph node (LN) draining the vaccination site and in distal LN draining the respiratory mucosa, although in lower numbers. At 29 dpv, a significant switch to IgG1 was clear in prescapular LN, while FMDV-specific ASC were detected in all lymphoid tissues draining the respiratory tract, mostly as IgM-secreting cells. None of the animals (n = 10) exhibited FMD symptoms after oronasal challenge at 30 dpv. Three days postinfection, a large increase in ASC numbers and rapid isotype switches to IgG1 were observed, particularly in LN-draining virus replication sites already described. These results indicate for the first time that systemic FMD vaccination in cattle effectively promotes the presence of anti-FMDV ASC in lymphoid tissues associated with the respiratory system. Oronasal infection triggered an immune reaction compatible with a local anamnestic response upon contact with the replicating FMDV, suggesting that FMD vaccination induces the circulation of virus-specific B lymphocytes, including memory B cells that differentiate into ASC soon after contact with the infective |
| format | info:ar-repo/semantics/artículo |
| id | INTA2536 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA25362018-06-01T14:22:46Z Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection Pega, Juan Franco Di Giacomo, Sebastián Bucafusco, Danilo Schammas, Juan Manuel Malacari, Darío Amilcar Barrionuevo, Florencia Mariel Capozzo, Alejandra Victoria Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Vacunación Anticuerpos Sistema Respiratorio Cattle Animal Diseases Foot and Mouth Disease Vaccination Antibodies Respiratory System Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, we studied the generation of antibody responses in local lymphoid tissues along the respiratory system in vaccinated and further aerosol-infected cattle. Animals immunized with a high-payload monovalent FMD vaccine developed high titers of neutralizing antibodies at 7 days postvaccination (dpv), reaching a plateau at 29 dpv. FMDV-specific antibody-secreting cells (ASC), predominantly IgM, were evident at 7 dpv in the prescapular lymph node (LN) draining the vaccination site and in distal LN draining the respiratory mucosa, although in lower numbers. At 29 dpv, a significant switch to IgG1 was clear in prescapular LN, while FMDV-specific ASC were detected in all lymphoid tissues draining the respiratory tract, mostly as IgM-secreting cells. None of the animals (n = 10) exhibited FMD symptoms after oronasal challenge at 30 dpv. Three days postinfection, a large increase in ASC numbers and rapid isotype switches to IgG1 were observed, particularly in LN-draining virus replication sites already described. These results indicate for the first time that systemic FMD vaccination in cattle effectively promotes the presence of anti-FMDV ASC in lymphoid tissues associated with the respiratory system. Oronasal infection triggered an immune reaction compatible with a local anamnestic response upon contact with the replicating FMDV, suggesting that FMD vaccination induces the circulation of virus-specific B lymphocytes, including memory B cells that differentiate into ASC soon after contact with the infective Instituto de Virología Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Barrionuevo, Florencia Mariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodríguez, L.L. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos Fil: Borca, Manuel Victor. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-06-01T14:19:36Z 2018-06-01T14:19:36Z 2015-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://jvi.asm.org/content/89/18/9581.abstract http://hdl.handle.net/20.500.12123/2536 0022-538X 1098-5514 http://dx.doi.org/10.1128/JVI.01082-15 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Virology 89 (18) : 9581-9590. (September 2015) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Vacunación Anticuerpos Sistema Respiratorio Cattle Animal Diseases Foot and Mouth Disease Vaccination Antibodies Respiratory System Pega, Juan Franco Di Giacomo, Sebastián Bucafusco, Danilo Schammas, Juan Manuel Malacari, Darío Amilcar Barrionuevo, Florencia Mariel Capozzo, Alejandra Victoria Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection |
| title | Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection |
| title_full | Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection |
| title_fullStr | Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection |
| title_full_unstemmed | Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection |
| title_short | Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection |
| title_sort | systemic foot and mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic responses upon aerosol infection |
| topic | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Vacunación Anticuerpos Sistema Respiratorio Cattle Animal Diseases Foot and Mouth Disease Vaccination Antibodies Respiratory System |
| url | http://jvi.asm.org/content/89/18/9581.abstract http://hdl.handle.net/20.500.12123/2536 http://dx.doi.org/10.1128/JVI.01082-15 |
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