Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://jvi.asm.org/content/87/5/2489.long http://hdl.handle.net/20.500.12123/2535 http://dx.doi.org/10.1128/JVI.02879-12 |
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| author | Pega, Juan Franco Bucafusco, Danilo Di Giacomo, Sebastián Schammas, Juan Manuel Malacari, Darío Amilcar Capozzo, Alejandra Victoria Arzt, J. Pérez-Beascoechea, C. Maradei, E. Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano |
| author_browse | Arzt, J. Borca, Manuel Victor Bucafusco, Danilo Capozzo, Alejandra Victoria Di Giacomo, Sebastián Malacari, Darío Amilcar Maradei, E. Pega, Juan Franco Perez Filgueira, Daniel Mariano Pérez-Beascoechea, C. Rodríguez, L.L. Schammas, Juan Manuel |
| author_facet | Pega, Juan Franco Bucafusco, Danilo Di Giacomo, Sebastián Schammas, Juan Manuel Malacari, Darío Amilcar Capozzo, Alejandra Victoria Arzt, J. Pérez-Beascoechea, C. Maradei, E. Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano |
| author_sort | Pega, Juan Franco |
| collection | INTA Digital |
| description | Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure. |
| format | Artículo |
| id | INTA2535 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA25352018-06-01T14:10:45Z Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle Pega, Juan Franco Bucafusco, Danilo Di Giacomo, Sebastián Schammas, Juan Manuel Malacari, Darío Amilcar Capozzo, Alejandra Victoria Arzt, J. Pérez-Beascoechea, C. Maradei, E. Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Respuesta Inmunológica Sistema Respiratorio Cattle Animal Diseases Foot and Mouth Disease Immune Response Respiratory System Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure. Instituto de Virología Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Arzt, J. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos Fil: Pérez Beascoeachea, C. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; Argentina Fil: Maradei, E. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; Argentina Fil: Rodríguez, L. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos Fil: Borca, Manuel Victor. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-06-01T13:49:23Z 2018-06-01T13:49:23Z 2013-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://jvi.asm.org/content/87/5/2489.long http://hdl.handle.net/20.500.12123/2535 0022-538X 1098-5514 http://dx.doi.org/10.1128/JVI.02879-12 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Virology 87 (5) : 2489-2495. (March 2013) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Respuesta Inmunológica Sistema Respiratorio Cattle Animal Diseases Foot and Mouth Disease Immune Response Respiratory System Pega, Juan Franco Bucafusco, Danilo Di Giacomo, Sebastián Schammas, Juan Manuel Malacari, Darío Amilcar Capozzo, Alejandra Victoria Arzt, J. Pérez-Beascoechea, C. Maradei, E. Rodríguez, L.L. Borca, Manuel Victor Perez Filgueira, Daniel Mariano Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle |
| title | Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle |
| title_full | Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle |
| title_fullStr | Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle |
| title_full_unstemmed | Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle |
| title_short | Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle |
| title_sort | early adaptive immune responses in the respiratory tract of foot and mouth disease virus infected cattle |
| topic | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Respuesta Inmunológica Sistema Respiratorio Cattle Animal Diseases Foot and Mouth Disease Immune Response Respiratory System |
| url | http://jvi.asm.org/content/87/5/2489.long http://hdl.handle.net/20.500.12123/2535 http://dx.doi.org/10.1128/JVI.02879-12 |
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