Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles
Interferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ELISA was set up using 308 samples from naïve and vaccinated cattle. The assay w...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0042682214005315 http://hdl.handle.net/20.500.12123/2516 https://doi.org/10.1016/j.virol.2014.11.023 |
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| author | Bucafusco, Danilo Di Giacomo, Sebastián Pega, Juan Franco Schammas, Juan Manuel Cardoso, Nancy Patricia Capozzo, Alejandra Victoria Perez Filgueira, Daniel Mariano |
| author_browse | Bucafusco, Danilo Capozzo, Alejandra Victoria Cardoso, Nancy Patricia Di Giacomo, Sebastián Pega, Juan Franco Perez Filgueira, Daniel Mariano Schammas, Juan Manuel |
| author_facet | Bucafusco, Danilo Di Giacomo, Sebastián Pega, Juan Franco Schammas, Juan Manuel Cardoso, Nancy Patricia Capozzo, Alejandra Victoria Perez Filgueira, Daniel Mariano |
| author_sort | Bucafusco, Danilo |
| collection | INTA Digital |
| description | Interferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ELISA was set up using 308 samples from naïve and vaccinated cattle. The assay was used to study cross-reactive responses between different FMDV vaccine strains. Blood samples from cattle immunized with monovalent vaccines containing A24/Cruzeiro/Brazil/55, A/Argentina/2001 or O1/Campos/Brazil/58 strains were tested using purified-inactivated FMDV from homologous and heterologous strains. A24/Cruzeiro was the most efficient IFN-γ inducer in all vaccinated animals, both when included in the vaccine or as stimulating antigen. We demonstrate that this was mainly due to the structural stability of the whole viral particle. These results show that IFN-γ production relies on the presence of 140S particles that can maintain their integrity along the incubation process in vitro, and throughout the vaccine´s shelf-life, when used in vivo. |
| format | Artículo |
| id | INTA2516 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA25162018-05-30T14:15:56Z Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles Bucafusco, Danilo Di Giacomo, Sebastián Pega, Juan Franco Schammas, Juan Manuel Cardoso, Nancy Patricia Capozzo, Alejandra Victoria Perez Filgueira, Daniel Mariano Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Vacunación Interferonas Cattle Animal Diseases Foot and Mouth Disease Vaccination Interferons Interferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ELISA was set up using 308 samples from naïve and vaccinated cattle. The assay was used to study cross-reactive responses between different FMDV vaccine strains. Blood samples from cattle immunized with monovalent vaccines containing A24/Cruzeiro/Brazil/55, A/Argentina/2001 or O1/Campos/Brazil/58 strains were tested using purified-inactivated FMDV from homologous and heterologous strains. A24/Cruzeiro was the most efficient IFN-γ inducer in all vaccinated animals, both when included in the vaccine or as stimulating antigen. We demonstrate that this was mainly due to the structural stability of the whole viral particle. These results show that IFN-γ production relies on the presence of 140S particles that can maintain their integrity along the incubation process in vitro, and throughout the vaccine´s shelf-life, when used in vivo. Instituto de Virología Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-05-30T14:14:43Z 2018-05-30T14:14:43Z 2015-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0042682214005315 http://hdl.handle.net/20.500.12123/2516 0042-6822 https://doi.org/10.1016/j.virol.2014.11.023 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Virology 476 : 11-18 (February 2015) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Vacunación Interferonas Cattle Animal Diseases Foot and Mouth Disease Vaccination Interferons Bucafusco, Danilo Di Giacomo, Sebastián Pega, Juan Franco Schammas, Juan Manuel Cardoso, Nancy Patricia Capozzo, Alejandra Victoria Perez Filgueira, Daniel Mariano Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles |
| title | Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles |
| title_full | Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles |
| title_fullStr | Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles |
| title_full_unstemmed | Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles |
| title_short | Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles |
| title_sort | foot and mouth disease vaccination induces cross reactive ifn γ responses in cattle that are dependent on the integrity of the 140s particles |
| topic | Ganado Bovino Enfermedades de los Animales Fiebre Aftosa Vacunación Interferonas Cattle Animal Diseases Foot and Mouth Disease Vaccination Interferons |
| url | https://www.sciencedirect.com/science/article/pii/S0042682214005315 http://hdl.handle.net/20.500.12123/2516 https://doi.org/10.1016/j.virol.2014.11.023 |
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