Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine
Internal exposure of the world’s most used herbicide glyphosate and its environmental metabolite AMPA is commonly assessed by analysis of urine, while excretion is mostly through feces. In this study, we explore the feasibility and effectiveness of feces as an alternative matrix for urine for biomon...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2026
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/24999 https://www.sciencedirect.com/science/article/pii/S016041202500772X https://doi.org/10.1016/j.envint.2025.110021 |
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| author | Dias, Jonatan Yen, Ling Alcon, Francisco Contreras, Josefa Abrantes, Nelson Campos, Isabel Baldi, Isabelle Bureau, Mathilde Christ, Florian Mandrioli, Daniele Sgargi, Daria Pasković, Igor Polić Pasković, Marija Glavan, Matjaž Hofman, Jakub Huerta Lwanga, Esperanza Harkes, Paula Norgaard, Trine Aparicio, Virginia Carolina Silva, Vera Schlünssen, Vivi Alaoui, Abdallah Scheepers, Paul Mol, Hans |
| author_browse | Abrantes, Nelson Alaoui, Abdallah Alcon, Francisco Aparicio, Virginia Carolina Baldi, Isabelle Bureau, Mathilde Campos, Isabel Christ, Florian Contreras, Josefa Dias, Jonatan Glavan, Matjaž Harkes, Paula Hofman, Jakub Huerta Lwanga, Esperanza Mandrioli, Daniele Mol, Hans Norgaard, Trine Pasković, Igor Polić Pasković, Marija Scheepers, Paul Schlünssen, Vivi Sgargi, Daria Silva, Vera Yen, Ling |
| author_facet | Dias, Jonatan Yen, Ling Alcon, Francisco Contreras, Josefa Abrantes, Nelson Campos, Isabel Baldi, Isabelle Bureau, Mathilde Christ, Florian Mandrioli, Daniele Sgargi, Daria Pasković, Igor Polić Pasković, Marija Glavan, Matjaž Hofman, Jakub Huerta Lwanga, Esperanza Harkes, Paula Norgaard, Trine Aparicio, Virginia Carolina Silva, Vera Schlünssen, Vivi Alaoui, Abdallah Scheepers, Paul Mol, Hans |
| author_sort | Dias, Jonatan |
| collection | INTA Digital |
| description | Internal exposure of the world’s most used herbicide glyphosate and its environmental metabolite AMPA is commonly assessed by analysis of urine, while excretion is mostly through feces. In this study, we explore the feasibility and effectiveness of feces as an alternative matrix for urine for biomonitoring of these two compounds. A method for the determination of polar pesticides was validated and applied for analysis of 716 human and 249 animal feces samples. The samples were collected in 2021, at study sites in ten European countries and one in Argentina. Detection frequencies (DF) and median concentrations (MC) observed in sub-populations (conventional farmers, organic farmers, neighbors (rural), consumers (not involved in agricultural activities) were compared. Glyphosate was rather common in human feces in 71 % of the samples from the European sites and in 100 % of Argentinean samples. Detection in feces was more frequent than in the corresponding urine samples (35 % for Europe, 86 % for Argentina). MC in feces were 17.6 µg/kg (Europe) and 153 µg/kg (Argentina). Variation in DF and MC between study sites was larger than between pooled sub-populations of all study sites. In farm animals, glyphosate was found in the majority of the feces samples. AMPA was found less frequently, depending on the species and farming system. High concentrations of glyphosate and AMPA in bat feces showed
that exposure also occurs at higher trophic levels in ecosystems near the investigated sites. In conclusion, analysis of feces reveals widespread exposure of both humans and animals to glyphosate, wider than so far reported based on urine as matrix. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24999 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA249992026-01-16T18:05:28Z Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine Dias, Jonatan Yen, Ling Alcon, Francisco Contreras, Josefa Abrantes, Nelson Campos, Isabel Baldi, Isabelle Bureau, Mathilde Christ, Florian Mandrioli, Daniele Sgargi, Daria Pasković, Igor Polić Pasković, Marija Glavan, Matjaž Hofman, Jakub Huerta Lwanga, Esperanza Harkes, Paula Norgaard, Trine Aparicio, Virginia Carolina Silva, Vera Schlünssen, Vivi Alaoui, Abdallah Scheepers, Paul Mol, Hans Biomonitoring Glifosato Hez Orina Animales Género Humano Glyphosate Faeces Urine Animals Humans aminomethylphosphonic acid (AMPA) Internal exposure of the world’s most used herbicide glyphosate and its environmental metabolite AMPA is commonly assessed by analysis of urine, while excretion is mostly through feces. In this study, we explore the feasibility and effectiveness of feces as an alternative matrix for urine for biomonitoring of these two compounds. A method for the determination of polar pesticides was validated and applied for analysis of 716 human and 249 animal feces samples. The samples were collected in 2021, at study sites in ten European countries and one in Argentina. Detection frequencies (DF) and median concentrations (MC) observed in sub-populations (conventional farmers, organic farmers, neighbors (rural), consumers (not involved in agricultural activities) were compared. Glyphosate was rather common in human feces in 71 % of the samples from the European sites and in 100 % of Argentinean samples. Detection in feces was more frequent than in the corresponding urine samples (35 % for Europe, 86 % for Argentina). MC in feces were 17.6 µg/kg (Europe) and 153 µg/kg (Argentina). Variation in DF and MC between study sites was larger than between pooled sub-populations of all study sites. In farm animals, glyphosate was found in the majority of the feces samples. AMPA was found less frequently, depending on the species and farming system. High concentrations of glyphosate and AMPA in bat feces showed that exposure also occurs at higher trophic levels in ecosystems near the investigated sites. In conclusion, analysis of feces reveals widespread exposure of both humans and animals to glyphosate, wider than so far reported based on urine as matrix. EEA Balcarce Fil: Dias, Jonatan. Wageningen University & Research. Wageningen Food Safety Research (WFSR); Países Bajos Fil: Yen, Ling. Wageningen University & Research. Wageningen Food Safety Research (WFSR); Países Bajos Fil: Francisco, Alcon. Universidad Politécnica de Cartagena. Agricultural Engineering School; España Fil: Alcon, Francisco. Universidad Politécnica de Cartagena. Department of Business Economics; España Fil: Contreras, Josefa. Universidad Politécnica de Cartagena. Department Agricultural Engineering; España Fil: Abrantes, Nelson. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; Portugal Fil: Campos, Isabel. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Environment and Planning; Portugal Fil: Baldi, Isabelle. University of Bordeaux. Bordeaux Population Health Research Centre (BPH); Francia Fil: Baldi, Isabelle. Institut national de la santé et de la recherche médicale. Bordeaux Population Health Research Centre (BPH); Francia Fil: Bureau, Mathilde. University of Bordeaux. Bordeaux Population Health Research Centre (BPH); Francia Fil: Bureau, Mathilde. Institut national de la santé et de la recherche médicale. Bordeaux Population Health Research Centre (BPH); Francia Fil: Christ, Florian. University of Bern. Institute of Geography; Suiza Fil: Mandrioli, Daniele. Ramazzini Institute. Cesare Maltoni Cancer Research Center; Italia Fil: Sgargi, Daria. Ramazzini Institute. Cesare Maltoni Cancer Research Center; Italia Fil: Pasković, Igor. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia Fil: Polić Pasković, Marija. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia Fil: Glavan, Matjaž. University of Ljubljana. Biotechnical Faculty. Agronomy Department; Eslovenia Fil: Hofman, Jakub. Masaryk University. Faculty of Science. Research Centre for Toxic Compounds in the Environment (RECETOX); República Checa Fil: Huerta Lwanga, Esperanza. Wageningen University and Research. Soil Physics and Land Management Group; Países Bajos Fil: Harkes, Paula. Wageningen University and Research. Soil Physics and Land Management Group; Países Bajos Fil: Norgaard, Trine. Aarhus University. Department of Agroecology; Dinamarca Fil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil. Silva, Vera. Wageningen University and Research. Soil Physics and Land Management Group; Países Bajos Fil: Schlünssen, Vivi. Aarhus University. Danish Ramazzini Centre. Department of Public Health; Dinamarca Fil: Alaoui, Abdallah. University of Bern. Institute of Geography; Suiza Fil: Scheepers, Paul. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos Fil: Mol, Hans. Wageningen University & Research. Wageningen Food Safety Research (WFSR); Países Bajos 2026-01-16T18:01:38Z 2026-01-16T18:01:38Z 2026-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24999 https://www.sciencedirect.com/science/article/pii/S016041202500772X 1873-6750 (online) 0160-4120 (impreso) https://doi.org/10.1016/j.envint.2025.110021 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Environment International 207 : 110021. (January 2026) |
| spellingShingle | Biomonitoring Glifosato Hez Orina Animales Género Humano Glyphosate Faeces Urine Animals Humans aminomethylphosphonic acid (AMPA) Dias, Jonatan Yen, Ling Alcon, Francisco Contreras, Josefa Abrantes, Nelson Campos, Isabel Baldi, Isabelle Bureau, Mathilde Christ, Florian Mandrioli, Daniele Sgargi, Daria Pasković, Igor Polić Pasković, Marija Glavan, Matjaž Hofman, Jakub Huerta Lwanga, Esperanza Harkes, Paula Norgaard, Trine Aparicio, Virginia Carolina Silva, Vera Schlünssen, Vivi Alaoui, Abdallah Scheepers, Paul Mol, Hans Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine |
| title | Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine |
| title_full | Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine |
| title_fullStr | Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine |
| title_full_unstemmed | Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine |
| title_short | Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine |
| title_sort | large scale biomonitoring of glyphosate and ampa by analysis of human and animal feces and comparison with urine |
| topic | Biomonitoring Glifosato Hez Orina Animales Género Humano Glyphosate Faeces Urine Animals Humans aminomethylphosphonic acid (AMPA) |
| url | http://hdl.handle.net/20.500.12123/24999 https://www.sciencedirect.com/science/article/pii/S016041202500772X https://doi.org/10.1016/j.envint.2025.110021 |
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