Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study
Reproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4 (BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle in dustry, primarily through infertility, abortion, and impaired reproductive performance...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Español |
| Publicado: |
Elsevier
2026
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24998 https://doi.org/10.1016/j.virusres.2025.199653 |
| _version_ | 1855039043316744192 |
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| author | Andreoli, Valentina Lopez, Sofia Delgado, Santiago German Perez, Sandra Elizabeth Pereyra, Susana Beatriz Gonzalez Altamiranda, Erika Analía Romeo, Florencia Grolli, Stefano Verna, Andrea Elizabeth |
| author_browse | Andreoli, Valentina Delgado, Santiago German Gonzalez Altamiranda, Erika Analía Grolli, Stefano Lopez, Sofia Pereyra, Susana Beatriz Perez, Sandra Elizabeth Romeo, Florencia Verna, Andrea Elizabeth |
| author_facet | Andreoli, Valentina Lopez, Sofia Delgado, Santiago German Perez, Sandra Elizabeth Pereyra, Susana Beatriz Gonzalez Altamiranda, Erika Analía Romeo, Florencia Grolli, Stefano Verna, Andrea Elizabeth |
| author_sort | Andreoli, Valentina |
| collection | INTA Digital |
| description | Reproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4
(BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle in
dustry, primarily through infertility, abortion, and impaired reproductive performance. Owing to the limited
efficacy of current antiviral strategies, this study evaluated the in vitro effects of platelet-rich plasma (PRP), at
concentrations of 5 % and 10 %, on the replication kinetics of these viruses in Madin-Darby bovine kidney
(MDBK) cells and primary bovine endometrial stromal cells (BESc). PRP modulated viral replication in a virus-,
cell type-, and dose-related manner. In BoAHV-1.1-infected MDBK cells, 10 % PRP reduced extracellular titres
but increases intracellular accumulation, suggesting interference with viral egress. In BESc, both intra- and
extracellular titres decreased, consistent with a broader antiviral effect. For BoGHV-4 and BVDV, PRP induced
variable and time-dependent responses across cell types. These results demonstrate that PRP can influence
bovine viral replication dynamics in vitro and support further investigations into its mechanistic basis and in vivo
therapeutic potential. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24998 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA249982026-01-16T17:53:29Z Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study Andreoli, Valentina Lopez, Sofia Delgado, Santiago German Perez, Sandra Elizabeth Pereyra, Susana Beatriz Gonzalez Altamiranda, Erika Analía Romeo, Florencia Grolli, Stefano Verna, Andrea Elizabeth Ganado Bovino Virus Herpes Virus Bovino Plasma Sanguíneo Inmunomodulación Cattle Viruses Bovine Herpesvirus Blood Plasma Immunomodulation Reproductive viral diseases caused by Bovine alphaherpesvirus 1.1 (BoAHV-1.1), Bovine gam-maherpesvirus 4 (BoGHV-4), and Bovine viral diarrhoea virus (BVDV) impose a substantial economic burden on the cattle in dustry, primarily through infertility, abortion, and impaired reproductive performance. Owing to the limited efficacy of current antiviral strategies, this study evaluated the in vitro effects of platelet-rich plasma (PRP), at concentrations of 5 % and 10 %, on the replication kinetics of these viruses in Madin-Darby bovine kidney (MDBK) cells and primary bovine endometrial stromal cells (BESc). PRP modulated viral replication in a virus-, cell type-, and dose-related manner. In BoAHV-1.1-infected MDBK cells, 10 % PRP reduced extracellular titres but increases intracellular accumulation, suggesting interference with viral egress. In BESc, both intra- and extracellular titres decreased, consistent with a broader antiviral effect. For BoGHV-4 and BVDV, PRP induced variable and time-dependent responses across cell types. These results demonstrate that PRP can influence bovine viral replication dynamics in vitro and support further investigations into its mechanistic basis and in vivo therapeutic potential. EEA Balcarce Fil: Fil: Andreoli, Valentina. University of Parma. Department of Veterinary Medical Science; Italia Fil: Lopez, Sofia. Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT); Argentina Fil: Lopez, Sofia. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Delgado, Santiago German. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Agrarias; Argentina Fil: Perez, Sandra Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Perez, Sandra Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Gonzalez Altamiranda, Erika Analía. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Romeo, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Grolli, Stefano. University of Parma. Department of Veterinary Medical Science; Italia Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce, Argentina Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina 2026-01-16T17:49:07Z 2026-01-16T17:49:07Z 2025-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24998 0168-1702 https://doi.org/10.1016/j.virusres.2025.199653 spa info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Virus Research 361 : 199653 (November 2025) |
| spellingShingle | Ganado Bovino Virus Herpes Virus Bovino Plasma Sanguíneo Inmunomodulación Cattle Viruses Bovine Herpesvirus Blood Plasma Immunomodulation Andreoli, Valentina Lopez, Sofia Delgado, Santiago German Perez, Sandra Elizabeth Pereyra, Susana Beatriz Gonzalez Altamiranda, Erika Analía Romeo, Florencia Grolli, Stefano Verna, Andrea Elizabeth Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study |
| title | Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study |
| title_full | Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study |
| title_fullStr | Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study |
| title_full_unstemmed | Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study |
| title_short | Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study |
| title_sort | modulatory effects of platelet rich plasma on viral kinetics of boahv 1 1 boghv 4 and bvdv in bovine cell cultures a proof of concept study |
| topic | Ganado Bovino Virus Herpes Virus Bovino Plasma Sanguíneo Inmunomodulación Cattle Viruses Bovine Herpesvirus Blood Plasma Immunomodulation |
| url | http://hdl.handle.net/20.500.12123/24998 https://doi.org/10.1016/j.virusres.2025.199653 |
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