Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis

Dietary uptake is estimated using residue data from food commodities and predicting the effect of food processing, such as peeling. The aim of this study was to explore the value of the analysis of duplicate portions supplemented by the analysis of urinary metabolites. Forty-three participants, cons...

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Autores principales: Wieland, Nina, Figueiredo, Daniel, Mol, Hans, González, Neus, Abrantes, Nelson, Aparicio, Virginia Carolina, Campos, Isabel, Contreras, Josefina, Francisco, Alcon, Glavan, Matjaž, Blagus, Tanja, Dolžan, Vita, Harkes, Paula, Nørgaard, Trine, Schlünssen, Vivi, Pasković, Igor, Polić Pasković, Marija, Graumans, Martien, Ragas, Ad, Russel, Frans, Scheepers, Paul
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2026
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/24996
https://www.sciencedirect.com/science/article/pii/S0273230025002041?via%3Dihub
https://doi.org/10.1016/j.yrtph.2025.105972
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author Wieland, Nina
Figueiredo, Daniel
Mol, Hans
González, Neus
Abrantes, Nelson
Aparicio, Virginia Carolina
Campos, Isabel
Contreras, Josefina
Francisco, Alcon
Glavan, Matjaž
Blagus, Tanja
Dolžan, Vita
Harkes, Paula
Nørgaard, Trine
Schlünssen, Vivi
Pasković, Igor
Polić Pasković, Marija
Graumans, Martien
Ragas, Ad
Russel, Frans
Scheepers, Paul
author_browse Abrantes, Nelson
Aparicio, Virginia Carolina
Blagus, Tanja
Campos, Isabel
Contreras, Josefina
Dolžan, Vita
Figueiredo, Daniel
Francisco, Alcon
Glavan, Matjaž
González, Neus
Graumans, Martien
Harkes, Paula
Mol, Hans
Nørgaard, Trine
Pasković, Igor
Polić Pasković, Marija
Ragas, Ad
Russel, Frans
Scheepers, Paul
Schlünssen, Vivi
Wieland, Nina
author_facet Wieland, Nina
Figueiredo, Daniel
Mol, Hans
González, Neus
Abrantes, Nelson
Aparicio, Virginia Carolina
Campos, Isabel
Contreras, Josefina
Francisco, Alcon
Glavan, Matjaž
Blagus, Tanja
Dolžan, Vita
Harkes, Paula
Nørgaard, Trine
Schlünssen, Vivi
Pasković, Igor
Polić Pasković, Marija
Graumans, Martien
Ragas, Ad
Russel, Frans
Scheepers, Paul
author_sort Wieland, Nina
collection INTA Digital
description Dietary uptake is estimated using residue data from food commodities and predicting the effect of food processing, such as peeling. The aim of this study was to explore the value of the analysis of duplicate portions supplemented by the analysis of urinary metabolites. Forty-three participants, consuming organic and nonorganic diets collected a duplicate portion of food and beverages for 24 h. Urine was collected up to 36 h to account for metabolism and prolonged excretion of metabolites. Pesticide residues were analysed in food portions and urine samples using LC-MS/MS and GC-MS/MS. Multiple pesticide residues were detected per food portion. Out of 183 pesticides, 86 were detected in the diet. Glyphosate and AMPA were detected in 42 % and 30 % of all urine samples, respectively, while detection in the diet was low. A positive relationship between dietary intake and urinary excretion was found for 2,4-D and MCPA. No diet-urine relationship was observed for glyphosate and AMPA, indicating contribution from external routes of exposure. Hazard index (HI) indicated no exposure above the acceptable daily intake (ADI) for all participants. This study demonstrates how DPA combined with urine analysis gives insight into contribution of diet to total pesticide exposure compared to standard monitoring.
format Artículo
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
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publishDateRange 2026
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publisherStr Elsevier
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spelling INTA249962026-01-16T14:56:53Z Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis Wieland, Nina Figueiredo, Daniel Mol, Hans González, Neus Abrantes, Nelson Aparicio, Virginia Carolina Campos, Isabel Contreras, Josefina Francisco, Alcon Glavan, Matjaž Blagus, Tanja Dolžan, Vita Harkes, Paula Nørgaard, Trine Schlünssen, Vivi Pasković, Igor Polić Pasković, Marija Graumans, Martien Ragas, Ad Russel, Frans Scheepers, Paul Plaguicidas Dieta Orinación Ración Diaria Aceptable Pesticides Diet Urination Acceptable Daily Intake Dietary Pesticide Urinary Excretion Dietary Exposure Duplicate Portion Analysis Dietary uptake is estimated using residue data from food commodities and predicting the effect of food processing, such as peeling. The aim of this study was to explore the value of the analysis of duplicate portions supplemented by the analysis of urinary metabolites. Forty-three participants, consuming organic and nonorganic diets collected a duplicate portion of food and beverages for 24 h. Urine was collected up to 36 h to account for metabolism and prolonged excretion of metabolites. Pesticide residues were analysed in food portions and urine samples using LC-MS/MS and GC-MS/MS. Multiple pesticide residues were detected per food portion. Out of 183 pesticides, 86 were detected in the diet. Glyphosate and AMPA were detected in 42 % and 30 % of all urine samples, respectively, while detection in the diet was low. A positive relationship between dietary intake and urinary excretion was found for 2,4-D and MCPA. No diet-urine relationship was observed for glyphosate and AMPA, indicating contribution from external routes of exposure. Hazard index (HI) indicated no exposure above the acceptable daily intake (ADI) for all participants. This study demonstrates how DPA combined with urine analysis gives insight into contribution of diet to total pesticide exposure compared to standard monitoring. EEA Balcarce Fil: Wieland, Nina. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos Fil: Figueiredo, Daniel. Utrecht University. Institute for Risk Assessment Sciences (IRAS); Países Bajos Fil: Mol, Hans. Wageningen University & Research. Wageningen Food Safety Research (WFSR); Países Bajos Fil: González, Neus. Universitat Rovira i Virgili. Institut d’Investigació Sanitària Pere Virgili (IISPV). Laboratory of Toxicology and Environmental Health; España Fil: Abrantes, Nelson. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; Portugal Fil: Aparicio, Virginia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Campos, Isabel. University of Aveiro. Centre for Environmental and Marine Studies (CESAM). Department of Biology; Portugal Fil: Contreras, Josefina. Universidad Politécnica de Cartagena. Agricultural Engineering School; España Fil: Francisco, Alcon. Universidad Politécnica de Cartagena. Agricultural Engineering School; España Fil: Glavan, Matjaž. University of Ljubljana. Biotechnical Faculty. Agronomy Department; Eslovenia Fil: Blagus, Tanja. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; Eslovenia Fil. Dolžan, Vita. University of Ljubljana. Faculty of Medicine. Institute of Biochemistry and Molecular Genetics. Pharmacogenetics Laboratory; Eslovenia Fil: Harkes, Paula. Wageningen University & Research (WUR). Soil Physics and Land Management Group; Países Bajos Fil: Nørgaard, Trine. Aarhus University. Danish Ramazzini Centre. Department of Public Health; Dinamarca Fil: Schlünssen, Vivi. Aarhus University. Danish Ramazzini Centre. Department of Public Health; Dinamarca Fil: Pasković, Igor. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia Fil: Polić Pasković, Marija. Institute of Agriculture and Tourism. Department of Agriculture and Nutrition; Croacia Fil: Graumans, Martien. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos Fil: Ragas, Ad. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos Fil: Russel, Frans. Radboud University. Division of Pharmacology and Toxicology. Department of Pharmacy; Países Bajos Fil: Scheepers, Paul. Radboud University. Radboud Institute for Biological and Environmental Sciences (RIBES). Department of Environmental Science; Países Bajos 2026-01-16T14:52:34Z 2026-01-16T14:52:34Z 2026-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24996 https://www.sciencedirect.com/science/article/pii/S0273230025002041?via%3Dihub 0273-2300 (impreso) 1096-0295 (online) https://doi.org/10.1016/j.yrtph.2025.105972 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Regulatory Toxicology and Pharmacology 164 : 105972. (January 2026)
spellingShingle Plaguicidas
Dieta
Orinación
Ración Diaria Aceptable
Pesticides
Diet
Urination
Acceptable Daily Intake
Dietary Pesticide
Urinary Excretion
Dietary Exposure
Duplicate Portion Analysis
Wieland, Nina
Figueiredo, Daniel
Mol, Hans
González, Neus
Abrantes, Nelson
Aparicio, Virginia Carolina
Campos, Isabel
Contreras, Josefina
Francisco, Alcon
Glavan, Matjaž
Blagus, Tanja
Dolžan, Vita
Harkes, Paula
Nørgaard, Trine
Schlünssen, Vivi
Pasković, Igor
Polić Pasković, Marija
Graumans, Martien
Ragas, Ad
Russel, Frans
Scheepers, Paul
Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis
title Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis
title_full Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis
title_fullStr Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis
title_full_unstemmed Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis
title_short Relationship between dietary pesticide intake and urinary excretion: a pilot study using duplicate portion analysis
title_sort relationship between dietary pesticide intake and urinary excretion a pilot study using duplicate portion analysis
topic Plaguicidas
Dieta
Orinación
Ración Diaria Aceptable
Pesticides
Diet
Urination
Acceptable Daily Intake
Dietary Pesticide
Urinary Excretion
Dietary Exposure
Duplicate Portion Analysis
url http://hdl.handle.net/20.500.12123/24996
https://www.sciencedirect.com/science/article/pii/S0273230025002041?via%3Dihub
https://doi.org/10.1016/j.yrtph.2025.105972
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