Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams
Brucella ovis (B. ovis) is the etiological agent of ram-contagious epididymitis, the leading cause of reproductive disorders in flocks worldwide. Although the attenuated B. melitensis Rev.1 strain gives heterologous protection against this pathogen, it has important disadvantages. Subunit vaccin...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2026
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24988 https://doi.org/10.1016/j.vetimm.2025.110997 |
| _version_ | 1855039041654751232 |
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| author | Moran, Maria Celeste Tammone Santos, Agostina Dominguez, Paula Moriones, Lucila Nieto Farias, María Victoria Maté, Laura Garcia, Juan Agustin Kuhn, Tobias Paolicchi, Fernando Fiorentino, María Andrea Rodriguez, Marcelo Gaston García, Jorge Pablo Cacciato, Claudio Santiago Goldbaum, Fernando Alberto Zylberman, Vanesa Pardo, Romina Paola Foscaldi, Sabrina Lützelschwab, Claudia María Lupi, Giuliana Marcipar, Ivan Santiago Estein, Silvia Marcela |
| author_browse | Cacciato, Claudio Santiago Dominguez, Paula Estein, Silvia Marcela Fiorentino, María Andrea Foscaldi, Sabrina Garcia, Juan Agustin García, Jorge Pablo Goldbaum, Fernando Alberto Kuhn, Tobias Lupi, Giuliana Lützelschwab, Claudia María Marcipar, Ivan Santiago Maté, Laura Moran, Maria Celeste Moriones, Lucila Nieto Farias, María Victoria Paolicchi, Fernando Pardo, Romina Paola Rodriguez, Marcelo Gaston Tammone Santos, Agostina Zylberman, Vanesa |
| author_facet | Moran, Maria Celeste Tammone Santos, Agostina Dominguez, Paula Moriones, Lucila Nieto Farias, María Victoria Maté, Laura Garcia, Juan Agustin Kuhn, Tobias Paolicchi, Fernando Fiorentino, María Andrea Rodriguez, Marcelo Gaston García, Jorge Pablo Cacciato, Claudio Santiago Goldbaum, Fernando Alberto Zylberman, Vanesa Pardo, Romina Paola Foscaldi, Sabrina Lützelschwab, Claudia María Lupi, Giuliana Marcipar, Ivan Santiago Estein, Silvia Marcela |
| author_sort | Moran, Maria Celeste |
| collection | INTA Digital |
| description | Brucella ovis (B. ovis) is the etiological agent of ram-contagious epididymitis, the leading cause of reproductive
disorders in flocks worldwide. Although the attenuated B. melitensis Rev.1 strain gives heterologous protection
against this pathogen, it has important disadvantages. Subunit vaccines could provide a safer alternative that
considers the One Health approach. Polymeric BLSOmp31 was previously identified as a protective immunogen
against this pathogen. In our previous work in BALB/c mice, we evaluated the performance of BLSOmp31
formulated in a new cage-like particle adjuvant called ISPA. In the present study, we administered BLSOmp31,
which was formulated in a new low-cost variant of ISPA called ISPA YOLK (BLSOmp31/ISPA YOLK). This
formulation was given to rams through both subcutaneous and ocular routes. We evaluated the systemic and
mucosal immune responses and assessed its protective capacity against B. ovis. BLSOmp31/ISPA YOLK admin istered by both routes induced systemic and variable mucosal IgG and IgA antibody response, without inter ference in the serological diagnosis. Additionally, this formulation induced significant specific cellular immune
responses and an increase in the relative expression levels of cytokine genes in peripheral blood mononuclear
cells with a mixed Th1/Th2 profile. While this vaccine did not prevent experimental infection with B. ovis,
parenterally immunized rams had fewer infected organs and less severe histopathological changes in repro ductive organs compared to animals vaccinated by ocular route and non-immunized rams. In contrast, this
formulation, whether administered by SC or CONJ route could reduce the elimination of B. ovis through semen,
and minimize the risk of spreading the infection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24988 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA249882026-01-14T23:24:42Z Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams Moran, Maria Celeste Tammone Santos, Agostina Dominguez, Paula Moriones, Lucila Nieto Farias, María Victoria Maté, Laura Garcia, Juan Agustin Kuhn, Tobias Paolicchi, Fernando Fiorentino, María Andrea Rodriguez, Marcelo Gaston García, Jorge Pablo Cacciato, Claudio Santiago Goldbaum, Fernando Alberto Zylberman, Vanesa Pardo, Romina Paola Foscaldi, Sabrina Lützelschwab, Claudia María Lupi, Giuliana Marcipar, Ivan Santiago Estein, Silvia Marcela Enfermedad Bacteriana Epididimitis Infecciosa Ovina Brucella ovis Bacterial Diseases Ovine Infectious Epididymitis Epididimitis Contagiosa de Carneros Inmunógeno Antígeno Polimérico BLSOmp31 Brucella ovis (B. ovis) is the etiological agent of ram-contagious epididymitis, the leading cause of reproductive disorders in flocks worldwide. Although the attenuated B. melitensis Rev.1 strain gives heterologous protection against this pathogen, it has important disadvantages. Subunit vaccines could provide a safer alternative that considers the One Health approach. Polymeric BLSOmp31 was previously identified as a protective immunogen against this pathogen. In our previous work in BALB/c mice, we evaluated the performance of BLSOmp31 formulated in a new cage-like particle adjuvant called ISPA. In the present study, we administered BLSOmp31, which was formulated in a new low-cost variant of ISPA called ISPA YOLK (BLSOmp31/ISPA YOLK). This formulation was given to rams through both subcutaneous and ocular routes. We evaluated the systemic and mucosal immune responses and assessed its protective capacity against B. ovis. BLSOmp31/ISPA YOLK admin istered by both routes induced systemic and variable mucosal IgG and IgA antibody response, without inter ference in the serological diagnosis. Additionally, this formulation induced significant specific cellular immune responses and an increase in the relative expression levels of cytokine genes in peripheral blood mononuclear cells with a mixed Th1/Th2 profile. While this vaccine did not prevent experimental infection with B. ovis, parenterally immunized rams had fewer infected organs and less severe histopathological changes in repro ductive organs compared to animals vaccinated by ocular route and non-immunized rams. In contrast, this formulation, whether administered by SC or CONJ route could reduce the elimination of B. ovis through semen, and minimize the risk of spreading the infection. EEA Balcarce Fil: Moran, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Inmunología; Argentina Fil: Moran, Maria Celeste. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Inmunología; Argentina Fil: Moran, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Microbiología Clínica y Experimental; Argentina Fil: Moran, Maria Celeste. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Microbiología Clínica y Experimental; Argentina Fil: Tammone Santos, Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Inmunología; Argentina Fil: Tammone Santos, Agostina. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Inmunología; Argentina Fil: Dominguez, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Inmunología; Argentina Fil: Dominguez, Paula. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Inmunología; Argentina Fil: Moriones, Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Inmunología; Argentina Fil: Moriones, Lucila. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Inmunología; Argentina Fil: Nieto Farias, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Virología; Argentina Fil: Nieto Farias, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Virología; Argentina Fil: Maté, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Farmacología; Argentina Fil: Maté, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Farmacología; Argentina Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Garcia, Juan Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Kuhn, Tobias. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Inmunología; Argentina Fil: Kuhn, Tobias. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Inmunología; Argentina Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Paolicchi, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Fiorentino, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Rodriguez, Marcelo Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Area de Bioestadística; Argentina Fil: Rodriguez, Marcelo Gaston. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Area de Bioestadística; Argentina Fil: García, Jorge Pablo. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Departamento de Clínica; Argentin Fil: Cacciato, Claudio Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Microbiología Clínica y Experimental; Argentina Fil: Cacciato, Claudio Santiago. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Microbiología Clínica y Experimental; Argentina Fil: Goldbaum, Fernando Alberto. Inmunova S.A.; Argentina Fil: Zylberman, Vanesa. Inmunova S.A.; Argentina Fil: Pardo, Romina Paola. Inmunova S.A.; Argentina Fil: Foscaldi, Sabrina. Inmunova S.A.; Argentina Fil: Lützelschwab, Claudia María. Swedish University of Agricultural Sciences. Department of Animal Biosciences; Suecia Fil: Lupi, Giuliana. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Marcipar, Ivan Santiago. Universidad Nacional del Litoral (UNL). Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Estein, Silvia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigación Veterinaria Tandil (CIVETAN). Laboratorio de Inmunología; Argentina Fil: Estein, Silvia Marcela. Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Facultad de Ciencias Veterinarias (FCV). Laboratorio de Inmunología; Argentina 2026-01-14T23:21:11Z 2026-01-14T23:21:11Z 2025-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24988 0165-2427 https://doi.org/10.1016/j.vetimm.2025.110997 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Immunology and Immunopathology 288, 110997 (September 2025) |
| spellingShingle | Enfermedad Bacteriana Epididimitis Infecciosa Ovina Brucella ovis Bacterial Diseases Ovine Infectious Epididymitis Epididimitis Contagiosa de Carneros Inmunógeno Antígeno Polimérico BLSOmp31 Moran, Maria Celeste Tammone Santos, Agostina Dominguez, Paula Moriones, Lucila Nieto Farias, María Victoria Maté, Laura Garcia, Juan Agustin Kuhn, Tobias Paolicchi, Fernando Fiorentino, María Andrea Rodriguez, Marcelo Gaston García, Jorge Pablo Cacciato, Claudio Santiago Goldbaum, Fernando Alberto Zylberman, Vanesa Pardo, Romina Paola Foscaldi, Sabrina Lützelschwab, Claudia María Lupi, Giuliana Marcipar, Ivan Santiago Estein, Silvia Marcela Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams |
| title | Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams |
| title_full | Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams |
| title_fullStr | Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams |
| title_full_unstemmed | Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams |
| title_short | Evaluation of the efficacy of polymeric antigen BLSOmp31 formulated in a new cage-like particle adjuvant (ISPA YOLK) administered by parenteral or mucosal routes against Brucella ovis in rams |
| title_sort | evaluation of the efficacy of polymeric antigen blsomp31 formulated in a new cage like particle adjuvant ispa yolk administered by parenteral or mucosal routes against brucella ovis in rams |
| topic | Enfermedad Bacteriana Epididimitis Infecciosa Ovina Brucella ovis Bacterial Diseases Ovine Infectious Epididymitis Epididimitis Contagiosa de Carneros Inmunógeno Antígeno Polimérico BLSOmp31 |
| url | http://hdl.handle.net/20.500.12123/24988 https://doi.org/10.1016/j.vetimm.2025.110997 |
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