Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes
Background: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintena...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2026
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24960 https://www.mdpi.com/2076-393X/14/1/36 https://doi.org/10.3390/vaccines14010036 |
| _version_ | 1855487431409664000 |
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| author | Sala, Juan Manuel Wilda, Maximiliano Miraglia, Maria Cruz Castillo, Mariangeles Perez Filgueira, Daniel Mariano Freire, Teresa Capozzo, Alejandra |
| author_browse | Capozzo, Alejandra Castillo, Mariangeles Freire, Teresa Miraglia, Maria Cruz Perez Filgueira, Daniel Mariano Sala, Juan Manuel Wilda, Maximiliano |
| author_facet | Sala, Juan Manuel Wilda, Maximiliano Miraglia, Maria Cruz Castillo, Mariangeles Perez Filgueira, Daniel Mariano Freire, Teresa Capozzo, Alejandra |
| author_sort | Sala, Juan Manuel |
| collection | INTA Digital |
| description | Background: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintenance in endemic settings, has not been investigated. Objectives: To evaluate the effect of natural F. hepatica infection on the magnitude and functional quality of the FMDV–specific antibody response in buffaloes under field conditions. Methods: Two buffalo herds (n = 50 each) were classified by infection status using coproparasitological analysis and serology. All animals were vaccinated within the national foot-and-mouth disease control programme, with the last dose administered 264 days before sampling. Serum neutralising titres, total antibodies by liquid-phase blocking ELISA, IgG levels, and IgG avidity to the A24/Cruzeiro vaccine strain were determined. Results: F. hepatica-infected buffaloes exhibited consistent decreases across all vaccine-induced antibody parameters. Neutralising titres were reduced approximately two-fold, IgG avidity by about 38 percent, IgG levels by about 36 percent, and liquid-phase blocking ELISA titres by about 1.6-fold compared with non-infected animals. Conclusions: This study provides the first field evidence that fasciolosis is associated with changes in the magnitude and quality of vaccine-induced humoral responses following FMDV vaccination in water buffaloes, indicating that F. hepatica infection may influence the interpretation of post-vaccination serological monitoring in this species under endemic field conditions. |
| format | Artículo |
| id | INTA24960 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA249602026-01-09T12:10:48Z Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes Sala, Juan Manuel Wilda, Maximiliano Miraglia, Maria Cruz Castillo, Mariangeles Perez Filgueira, Daniel Mariano Freire, Teresa Capozzo, Alejandra Fasciola hepatica Bubalus bubalis Water Buffaloes Animal Diseases Antibodies Vaccination Aphthovirus Immunomodulation Búfalo de Agua Enfermedades de los Animales Anticuerpos Vacunación Virus Fiebre Aftosa Inmunomodulación Foot and Mouth Disease Virus Background: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintenance in endemic settings, has not been investigated. Objectives: To evaluate the effect of natural F. hepatica infection on the magnitude and functional quality of the FMDV–specific antibody response in buffaloes under field conditions. Methods: Two buffalo herds (n = 50 each) were classified by infection status using coproparasitological analysis and serology. All animals were vaccinated within the national foot-and-mouth disease control programme, with the last dose administered 264 days before sampling. Serum neutralising titres, total antibodies by liquid-phase blocking ELISA, IgG levels, and IgG avidity to the A24/Cruzeiro vaccine strain were determined. Results: F. hepatica-infected buffaloes exhibited consistent decreases across all vaccine-induced antibody parameters. Neutralising titres were reduced approximately two-fold, IgG avidity by about 38 percent, IgG levels by about 36 percent, and liquid-phase blocking ELISA titres by about 1.6-fold compared with non-infected animals. Conclusions: This study provides the first field evidence that fasciolosis is associated with changes in the magnitude and quality of vaccine-induced humoral responses following FMDV vaccination in water buffaloes, indicating that F. hepatica infection may influence the interpretation of post-vaccination serological monitoring in this species under endemic field conditions. EEA Mercedes Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Sala, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Virología Humana y Animal; Argentina Fil: Wilda, Maximiliano. Universidad Abierta Interamericana. Centro de Virología Humana y Animal; Argentina Fil: Miraglia, María Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miraglia, María Cruz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Castillo, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castillo, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Perez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Freire, Teresa. Universidad de La República. Facultad de Medicina. Departamento de Inmunobiología. Laboratorio de Inmunomodulación y Vacunas; Uruguay Fil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capozzo, Alejandra. Universidad Abierta Interamericana. Centro de Altos Estudios en Ciencias Humanas y de la Salud; Argentina 2026-01-09T11:59:18Z 2026-01-09T11:59:18Z 2026-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24960 https://www.mdpi.com/2076-393X/14/1/36 2076-393X https://doi.org/10.3390/vaccines14010036 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Vaccines 14 (1) : 36. (January 2026) |
| spellingShingle | Fasciola hepatica Bubalus bubalis Water Buffaloes Animal Diseases Antibodies Vaccination Aphthovirus Immunomodulation Búfalo de Agua Enfermedades de los Animales Anticuerpos Vacunación Virus Fiebre Aftosa Inmunomodulación Foot and Mouth Disease Virus Sala, Juan Manuel Wilda, Maximiliano Miraglia, Maria Cruz Castillo, Mariangeles Perez Filgueira, Daniel Mariano Freire, Teresa Capozzo, Alejandra Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes |
| title | Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes |
| title_full | Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes |
| title_fullStr | Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes |
| title_full_unstemmed | Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes |
| title_short | Field Evidence of Fasciola hepatica-Mediated Modulation of Antibody Responses to Foot-and-Mouth Disease Vaccination in Buffaloes |
| title_sort | field evidence of fasciola hepatica mediated modulation of antibody responses to foot and mouth disease vaccination in buffaloes |
| topic | Fasciola hepatica Bubalus bubalis Water Buffaloes Animal Diseases Antibodies Vaccination Aphthovirus Immunomodulation Búfalo de Agua Enfermedades de los Animales Anticuerpos Vacunación Virus Fiebre Aftosa Inmunomodulación Foot and Mouth Disease Virus |
| url | http://hdl.handle.net/20.500.12123/24960 https://www.mdpi.com/2076-393X/14/1/36 https://doi.org/10.3390/vaccines14010036 |
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