Double antigen sandwich ELISA for detection of antibodies against Babesia bovis
Babesia bovis is a hemoparasite responsible for bovine babesiosis, an economically important tickborne disease widespread in tropical and subtropical regions of the world. Serological tests are used in calves to evaluate the need for vaccination and the immunity achieved after vaccination. We develo...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2026
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/24883 https://www.sciencedirect.com/science/article/abs/pii/S0304401725002687 https://doi.org/10.1016/j.vetpar.2025.110657 |
| _version_ | 1855487416694996992 |
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| author | Primo, María Evangelina Valentini, Beatriz Susana Mazzucco Panizza, Matilde Nahimé Morel, Nicolas Thompson, Carolina Soledad |
| author_browse | Mazzucco Panizza, Matilde Nahimé Morel, Nicolas Primo, María Evangelina Thompson, Carolina Soledad Valentini, Beatriz Susana |
| author_facet | Primo, María Evangelina Valentini, Beatriz Susana Mazzucco Panizza, Matilde Nahimé Morel, Nicolas Thompson, Carolina Soledad |
| author_sort | Primo, María Evangelina |
| collection | INTA Digital |
| description | Babesia bovis is a hemoparasite responsible for bovine babesiosis, an economically important tickborne disease widespread in tropical and subtropical regions of the world. Serological tests are used in calves to evaluate the need for vaccination and the immunity achieved after vaccination. We developed, validated, and evaluated under field conditions a double-antigen sandwich ELISA (dasELISA) using a recombinant major surface antigen 2c (MSA2c) from a pathogenic and an attenuated B. bovis strain. The tests were validated using a panel of 1057 serum samples of known status (217 known-positive and 840 known-negative). Samples from 116 herds (n = 2771) were evaluated by indirect ELISA (iELISA) and dasELISA. Samples with disagreement between ELISAs (231 out of 594, 38.9 %) were analyzed by IFAT. The dasELISA cutoff was ≥ 52 %P, with a diagnostic sensitivity of 94.5 % (95 % CI, 90.5–97.1) and a diagnostic specificity of 96.4 % (95 % CI, 94.9–97.6). The area under the curve (AUC) was assessed to be 0.979, which corresponds to an excellent ability of the assay to discriminate truly infected from truly uninfected animals. Concordance among dasELISA and iELISA was 78.6 %, with a κ value of 0.567 (95 % CI = 0.537–0.598); meanwhile, 57 % and 43 % of the IFAT results agreed with dasELISA and iELISA, respectively. The dasELISA could be useful for large-scale detection of anti–B. bovis antibodies in calves pre- and post-vaccination and for seroepidemiologic investigations, given its appropriate sensitivity and specificity and the simplicity of production. |
| format | Artículo |
| id | INTA24883 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA248832026-01-05T16:35:43Z Double antigen sandwich ELISA for detection of antibodies against Babesia bovis Primo, María Evangelina Valentini, Beatriz Susana Mazzucco Panizza, Matilde Nahimé Morel, Nicolas Thompson, Carolina Soledad Babesia bovis Antigens ELISA Antibodies Tick-borne Diseases Cattle Antígenos Anticuerpos Enfermedad Transmitida por Garrapatas Ganado Bovino Babesia bovis is a hemoparasite responsible for bovine babesiosis, an economically important tickborne disease widespread in tropical and subtropical regions of the world. Serological tests are used in calves to evaluate the need for vaccination and the immunity achieved after vaccination. We developed, validated, and evaluated under field conditions a double-antigen sandwich ELISA (dasELISA) using a recombinant major surface antigen 2c (MSA2c) from a pathogenic and an attenuated B. bovis strain. The tests were validated using a panel of 1057 serum samples of known status (217 known-positive and 840 known-negative). Samples from 116 herds (n = 2771) were evaluated by indirect ELISA (iELISA) and dasELISA. Samples with disagreement between ELISAs (231 out of 594, 38.9 %) were analyzed by IFAT. The dasELISA cutoff was ≥ 52 %P, with a diagnostic sensitivity of 94.5 % (95 % CI, 90.5–97.1) and a diagnostic specificity of 96.4 % (95 % CI, 94.9–97.6). The area under the curve (AUC) was assessed to be 0.979, which corresponds to an excellent ability of the assay to discriminate truly infected from truly uninfected animals. Concordance among dasELISA and iELISA was 78.6 %, with a κ value of 0.567 (95 % CI = 0.537–0.598); meanwhile, 57 % and 43 % of the IFAT results agreed with dasELISA and iELISA, respectively. The dasELISA could be useful for large-scale detection of anti–B. bovis antibodies in calves pre- and post-vaccination and for seroepidemiologic investigations, given its appropriate sensitivity and specificity and the simplicity of production. EEA Rafaela Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Valentini, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Mazzucco Panizza, Matilde Nahimé. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Morel, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina 2026-01-05T16:32:46Z 2026-01-05T16:32:46Z 2026-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24883 https://www.sciencedirect.com/science/article/abs/pii/S0304401725002687 0304-4017 1873-2550 https://doi.org/10.1016/j.vetpar.2025.110657 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Parasitology 342 : 110657. (February 2026) |
| spellingShingle | Babesia bovis Antigens ELISA Antibodies Tick-borne Diseases Cattle Antígenos Anticuerpos Enfermedad Transmitida por Garrapatas Ganado Bovino Primo, María Evangelina Valentini, Beatriz Susana Mazzucco Panizza, Matilde Nahimé Morel, Nicolas Thompson, Carolina Soledad Double antigen sandwich ELISA for detection of antibodies against Babesia bovis |
| title | Double antigen sandwich ELISA for detection of antibodies against Babesia bovis |
| title_full | Double antigen sandwich ELISA for detection of antibodies against Babesia bovis |
| title_fullStr | Double antigen sandwich ELISA for detection of antibodies against Babesia bovis |
| title_full_unstemmed | Double antigen sandwich ELISA for detection of antibodies against Babesia bovis |
| title_short | Double antigen sandwich ELISA for detection of antibodies against Babesia bovis |
| title_sort | double antigen sandwich elisa for detection of antibodies against babesia bovis |
| topic | Babesia bovis Antigens ELISA Antibodies Tick-borne Diseases Cattle Antígenos Anticuerpos Enfermedad Transmitida por Garrapatas Ganado Bovino |
| url | http://hdl.handle.net/20.500.12123/24883 https://www.sciencedirect.com/science/article/abs/pii/S0304401725002687 https://doi.org/10.1016/j.vetpar.2025.110657 |
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