Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin
Lactoferrin, a multifunctional glycoprotein with significant biological properties, presents significant potential for the prevention and treatment of infectious diseases. However, the effectiveness of oral Lactoferrin is limited by its susceptibility to degradation in harsh stomach conditions, redu...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2026
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24872 https://www.sciencedirect.com/science/article/abs/pii/S0141813025024213 https://doi.org/10.1016/j.ijbiomac.2025.141870 |
| _version_ | 1855039022951301120 |
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| author | Cots, Agustina Camacho, Nahuel Matías Palma, Santiago Daniel Alustiza, Fabrisio Eduardo Pedraza, María del Luján Bonino, Facundo Carreño, Joel Flores Bracamonte, Carolina Acevedo, Diego Bozzo, Andrea Bellingeri, Romina |
| author_browse | Acevedo, Diego Alustiza, Fabrisio Eduardo Bellingeri, Romina Bonino, Facundo Bozzo, Andrea Camacho, Nahuel Matías Carreño, Joel Cots, Agustina Flores Bracamonte, Carolina Palma, Santiago Daniel Pedraza, María del Luján |
| author_facet | Cots, Agustina Camacho, Nahuel Matías Palma, Santiago Daniel Alustiza, Fabrisio Eduardo Pedraza, María del Luján Bonino, Facundo Carreño, Joel Flores Bracamonte, Carolina Acevedo, Diego Bozzo, Andrea Bellingeri, Romina |
| author_sort | Cots, Agustina |
| collection | INTA Digital |
| description | Lactoferrin, a multifunctional glycoprotein with significant biological properties, presents significant potential for the prevention and treatment of infectious diseases. However, the effectiveness of oral Lactoferrin is limited by its susceptibility to degradation in harsh stomach conditions, reducing its bioavailability and therapeutic efficacy. To address this challenge, this study employs Chitosan/Alginate microparticles to enhance Lactoferrin stability and antibacterial activity. Microparticles were synthesized through external ionic gelation and thoroughly characterized. Chitosan/Alginate microparticles were significantly smaller than Alginate microparticles, with a high loading efficiency of 93.65 %. Fourier transform infrared (FT-IR) spectroscopy confirmed the successful incorporation of Lactoferrin. Release studies demonstrated minimal Lactoferrin release in simulated gastric fluid, while a controlled release was observed in simulated intestinal fluid. Notably encapsulated Lactoferrin retained its structural integrity after exposure to simulated gastrointestinal conditions. Antibacterial assay against Escherichia coli showed that Chitosan/Alginate microcapsules maintain Lactoferrin antibacterial activity after its passage through simulated gastrointestinal conditions. Furthermore, FT-IR spectroscopy and zeta-potential analysis provided novel insights into the mechanism of Lactoferrin's interaction with bacterial LPS, highlighting its ability to destabilize bacterial membrane of this Escherichia coli strain. These findings underscore the Chitosan/Alginate system as a promising strategy for enhancing the therapeutic potential of Lactoferrin. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24872 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA248722026-01-05T11:32:39Z Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin Cots, Agustina Camacho, Nahuel Matías Palma, Santiago Daniel Alustiza, Fabrisio Eduardo Pedraza, María del Luján Bonino, Facundo Carreño, Joel Flores Bracamonte, Carolina Acevedo, Diego Bozzo, Andrea Bellingeri, Romina Lactoferrina Quitosano Alginato Ganado Bovino Glicoproteínas Lactoferrin Chitosan Alginates Cattle Glycoproteins Lactoferrin, a multifunctional glycoprotein with significant biological properties, presents significant potential for the prevention and treatment of infectious diseases. However, the effectiveness of oral Lactoferrin is limited by its susceptibility to degradation in harsh stomach conditions, reducing its bioavailability and therapeutic efficacy. To address this challenge, this study employs Chitosan/Alginate microparticles to enhance Lactoferrin stability and antibacterial activity. Microparticles were synthesized through external ionic gelation and thoroughly characterized. Chitosan/Alginate microparticles were significantly smaller than Alginate microparticles, with a high loading efficiency of 93.65 %. Fourier transform infrared (FT-IR) spectroscopy confirmed the successful incorporation of Lactoferrin. Release studies demonstrated minimal Lactoferrin release in simulated gastric fluid, while a controlled release was observed in simulated intestinal fluid. Notably encapsulated Lactoferrin retained its structural integrity after exposure to simulated gastrointestinal conditions. Antibacterial assay against Escherichia coli showed that Chitosan/Alginate microcapsules maintain Lactoferrin antibacterial activity after its passage through simulated gastrointestinal conditions. Furthermore, FT-IR spectroscopy and zeta-potential analysis provided novel insights into the mechanism of Lactoferrin's interaction with bacterial LPS, highlighting its ability to destabilize bacterial membrane of this Escherichia coli strain. These findings underscore the Chitosan/Alginate system as a promising strategy for enhancing the therapeutic potential of Lactoferrin. EEA Marcos Juárez Fil: Cots, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina Fil: Cots, Agustina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina Fil: Camacho, Nahuel Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Camacho, Nahuel Matías. Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Palma, Santiago D. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Palma, Santiago D. Universidad Nacional de Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Pedraza, Lujan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Bonino, Facundo. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Ciencias Básicas; Argentina Fil: Carreño, Joel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Ciencias Básicas; Argentina Fil: Flores Bracamonte, Carolina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina Fil: Flores Bracamonte, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina Fil: Acevedo, Diego. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina Fil: Acevedo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina Fil: Bozzo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina Fil: Bozzo, Andrea. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina Fil: Bellingeri, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina 2026-01-05T11:29:11Z 2026-01-05T11:29:11Z 2025-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24872 https://www.sciencedirect.com/science/article/abs/pii/S0141813025024213 0141-8130 1879-0003 https://doi.org/10.1016/j.ijbiomac.2025.141870 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier International Journal of Biological Macromolecules 307 (Part 2) : 141870. (May 2025) |
| spellingShingle | Lactoferrina Quitosano Alginato Ganado Bovino Glicoproteínas Lactoferrin Chitosan Alginates Cattle Glycoproteins Cots, Agustina Camacho, Nahuel Matías Palma, Santiago Daniel Alustiza, Fabrisio Eduardo Pedraza, María del Luján Bonino, Facundo Carreño, Joel Flores Bracamonte, Carolina Acevedo, Diego Bozzo, Andrea Bellingeri, Romina Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin |
| title | Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin |
| title_full | Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin |
| title_fullStr | Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin |
| title_full_unstemmed | Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin |
| title_short | Chitosan-alginate microcapsules: A strategy for improving stability and antibacterial potential of bovine Lactoferrin |
| title_sort | chitosan alginate microcapsules a strategy for improving stability and antibacterial potential of bovine lactoferrin |
| topic | Lactoferrina Quitosano Alginato Ganado Bovino Glicoproteínas Lactoferrin Chitosan Alginates Cattle Glycoproteins |
| url | http://hdl.handle.net/20.500.12123/24872 https://www.sciencedirect.com/science/article/abs/pii/S0141813025024213 https://doi.org/10.1016/j.ijbiomac.2025.141870 |
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