Phylogeny and chromosomal differentiation in Cestreae (Solanaceae)
Cestreae is a monophyletic tribe within the subfamily Cestroideae (Solanaceae), comprising the American genera Cestrum, Sessea, and Vestia. While the monophyly of Cestrum has been confirmed by DNA sequences, the phylogenetic relationships among its species, as well as its relationship to Sessea and...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Wiley
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24806 https://onlinelibrary.wiley.com/doi/full/10.1002/tax.70049 https://doi.org/10.1002/tax.70049 |
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| author | Maldonado, Ludmila Hajduczyk Rutz, Jésica L. Yañez Santos, Anahí Mara Chiarini, Franco Urdampilleta, Juan D. |
| author_browse | Chiarini, Franco Hajduczyk Rutz, Jésica L. Maldonado, Ludmila Urdampilleta, Juan D. Yañez Santos, Anahí Mara |
| author_facet | Maldonado, Ludmila Hajduczyk Rutz, Jésica L. Yañez Santos, Anahí Mara Chiarini, Franco Urdampilleta, Juan D. |
| author_sort | Maldonado, Ludmila |
| collection | INTA Digital |
| description | Cestreae is a monophyletic tribe within the subfamily Cestroideae (Solanaceae), comprising the American genera Cestrum, Sessea, and Vestia. While the monophyly of Cestrum has been confirmed by DNA sequences, the phylogenetic relationships among its species, as well as its relationship to Sessea and Vestia remain unresolved. This study describes cytogenetic traits, expands cytological records, extends phylogenetic sampling, and analyzes chromosome evolution in Cestreae through ancestral character reconstruction. Chromosome counts, karyotype analyses, and B chromosome identification were conducted using root meristem preparations stained with Giemsa. Fluorescent in situ hybridization (FISH) with rDNA probes and DAPI was used to examine rDNA distribution. Phylogenetic relationships were inferred for 23 species using four molecular markers (ITS, matK, ndhF, trnL-F) under maximum likelihood and Bayesian inference approaches. In addition to sequences from GenBank/NCBI, 14 ITS sequences were newly obtained from PCR products. Ancestral haploid chromosome numbers (n) were reconstructed using ChromEvol, while karyotype formulae and rDNA distribution patterns were inferred through stochastic character mapping (SCM) in R. All analyzed species had a chromosome number of 2n = 16, with two types of karyotype formula. Three Cestrum species possessed B chromosomes, and four distinct rDNA distribution patterns were identified. Ancestral state reconstruction suggests that 2n = 16, a karyotype formula of 7m + 1sm, and rDNA synteny (18–5.8–26S + 5S) on the sm pair #8 and terminal 18–5.8–26S sites on one m chromosome pair, may represent ancestral characteristics in Cestreae. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24806 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA248062025-12-30T11:23:47Z Phylogeny and chromosomal differentiation in Cestreae (Solanaceae) Maldonado, Ludmila Hajduczyk Rutz, Jésica L. Yañez Santos, Anahí Mara Chiarini, Franco Urdampilleta, Juan D. Cestrum Solanaceae Phylogeny Karyotypes Ribosomal DNA Filogenia Cariotipos ADN Ribosomal rDNA Cestreae is a monophyletic tribe within the subfamily Cestroideae (Solanaceae), comprising the American genera Cestrum, Sessea, and Vestia. While the monophyly of Cestrum has been confirmed by DNA sequences, the phylogenetic relationships among its species, as well as its relationship to Sessea and Vestia remain unresolved. This study describes cytogenetic traits, expands cytological records, extends phylogenetic sampling, and analyzes chromosome evolution in Cestreae through ancestral character reconstruction. Chromosome counts, karyotype analyses, and B chromosome identification were conducted using root meristem preparations stained with Giemsa. Fluorescent in situ hybridization (FISH) with rDNA probes and DAPI was used to examine rDNA distribution. Phylogenetic relationships were inferred for 23 species using four molecular markers (ITS, matK, ndhF, trnL-F) under maximum likelihood and Bayesian inference approaches. In addition to sequences from GenBank/NCBI, 14 ITS sequences were newly obtained from PCR products. Ancestral haploid chromosome numbers (n) were reconstructed using ChromEvol, while karyotype formulae and rDNA distribution patterns were inferred through stochastic character mapping (SCM) in R. All analyzed species had a chromosome number of 2n = 16, with two types of karyotype formula. Three Cestrum species possessed B chromosomes, and four distinct rDNA distribution patterns were identified. Ancestral state reconstruction suggests that 2n = 16, a karyotype formula of 7m + 1sm, and rDNA synteny (18–5.8–26S + 5S) on the sm pair #8 and terminal 18–5.8–26S sites on one m chromosome pair, may represent ancestral characteristics in Cestreae. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Maldonado, Ludmila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Maldonado, Ludmila. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Hajduczyk Rutz, Jésica L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Hajduczyk Rutz, Jésica L. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Yañez Santos, Anahí Mara. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Yañez Santos, Anahí Mara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Chiarini, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Chiarini, Franco. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Urdampilleta, Juan D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Urdampilleta, Juan D. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina 2025-12-30T11:21:01Z 2025-12-30T11:21:01Z 2025-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24806 https://onlinelibrary.wiley.com/doi/full/10.1002/tax.70049 0040-0262 1996-8175 https://doi.org/10.1002/tax.70049 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Taxon : 1-14 (First published: 25 September 2025) |
| spellingShingle | Cestrum Solanaceae Phylogeny Karyotypes Ribosomal DNA Filogenia Cariotipos ADN Ribosomal rDNA Maldonado, Ludmila Hajduczyk Rutz, Jésica L. Yañez Santos, Anahí Mara Chiarini, Franco Urdampilleta, Juan D. Phylogeny and chromosomal differentiation in Cestreae (Solanaceae) |
| title | Phylogeny and chromosomal differentiation in Cestreae (Solanaceae) |
| title_full | Phylogeny and chromosomal differentiation in Cestreae (Solanaceae) |
| title_fullStr | Phylogeny and chromosomal differentiation in Cestreae (Solanaceae) |
| title_full_unstemmed | Phylogeny and chromosomal differentiation in Cestreae (Solanaceae) |
| title_short | Phylogeny and chromosomal differentiation in Cestreae (Solanaceae) |
| title_sort | phylogeny and chromosomal differentiation in cestreae solanaceae |
| topic | Cestrum Solanaceae Phylogeny Karyotypes Ribosomal DNA Filogenia Cariotipos ADN Ribosomal rDNA |
| url | http://hdl.handle.net/20.500.12123/24806 https://onlinelibrary.wiley.com/doi/full/10.1002/tax.70049 https://doi.org/10.1002/tax.70049 |
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