Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite’s rapid growth. Unfortunately, the T. parva transportome is still not...
| Autores principales: | , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24800 https://www.sciencedirect.com/science/article/pii/S1476927125003147 https://doi.org/10.1016/j.compbiolchem.2025.108653 |
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| author | Kotsovolos, Nikolaos Schnittger, Leonhard Sibeko-Matjila, Kgomotso |
| author_browse | Kotsovolos, Nikolaos Schnittger, Leonhard Sibeko-Matjila, Kgomotso |
| author_facet | Kotsovolos, Nikolaos Schnittger, Leonhard Sibeko-Matjila, Kgomotso |
| author_sort | Kotsovolos, Nikolaos |
| collection | INTA Digital |
| description | As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite’s rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative
annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24800 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA248002025-12-29T16:09:22Z Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins Kotsovolos, Nikolaos Schnittger, Leonhard Sibeko-Matjila, Kgomotso Bioinformática Theileria parva Proteína Aglutinante Enfermedades de los Animales Bioinformatics Binding Proteins Animal Diseases As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite’s rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa. Instituto de Patobiología Fil: Kotsovolos, Nikolaos. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Sibeko-Matjila, Kgomotso. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica 2025-12-29T16:04:35Z 2025-12-29T16:04:35Z 2026-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24800 https://www.sciencedirect.com/science/article/pii/S1476927125003147 1476-9271 1476-928X (online) https://doi.org/10.1016/j.compbiolchem.2025.108653 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Computational Biology and Chemistry 120 (1): 108653. (February 2026) |
| spellingShingle | Bioinformática Theileria parva Proteína Aglutinante Enfermedades de los Animales Bioinformatics Binding Proteins Animal Diseases Kotsovolos, Nikolaos Schnittger, Leonhard Sibeko-Matjila, Kgomotso Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins |
| title | Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins |
| title_full | Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins |
| title_fullStr | Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins |
| title_full_unstemmed | Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins |
| title_short | Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins |
| title_sort | sequence and structure based bioinformatic screening for potential theileria parva transport related proteins |
| topic | Bioinformática Theileria parva Proteína Aglutinante Enfermedades de los Animales Bioinformatics Binding Proteins Animal Diseases |
| url | http://hdl.handle.net/20.500.12123/24800 https://www.sciencedirect.com/science/article/pii/S1476927125003147 https://doi.org/10.1016/j.compbiolchem.2025.108653 |
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