Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://pubs.acs.org/doi/abs/10.1021/jf501575m http://hdl.handle.net/20.500.12123/2480 http://dx.doi.org/10.1021/jf501575m |
| _version_ | 1855483063892443136 |
|---|---|
| author | Muñoz, Claudio Javier Gomez Talquenca, Gonzalo Chialva, Constanza Soledad Ibáñez, Javier Martínez-Zapater, José M. Peña Neira, Alvaro Lijavetzky, Diego |
| author_browse | Chialva, Constanza Soledad Gomez Talquenca, Gonzalo Ibáñez, Javier Lijavetzky, Diego Martínez-Zapater, José M. Muñoz, Claudio Javier Peña Neira, Alvaro |
| author_facet | Muñoz, Claudio Javier Gomez Talquenca, Gonzalo Chialva, Constanza Soledad Ibáñez, Javier Martínez-Zapater, José M. Peña Neira, Alvaro Lijavetzky, Diego |
| author_sort | Muñoz, Claudio Javier |
| collection | INTA Digital |
| description | Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation. |
| format | Artículo |
| id | INTA2480 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA24802020-01-07T14:33:08Z Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones Muñoz, Claudio Javier Gomez Talquenca, Gonzalo Chialva, Constanza Soledad Ibáñez, Javier Martínez-Zapater, José M. Peña Neira, Alvaro Lijavetzky, Diego Vid Variedades Clones Expresión Génica Antocianinas Grapevines Varieties Gene Expression Anthocyanins Malbec Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation. EEA Mendoza Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina Fil: Chialva, Constanza Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Ibáñez, Javier. Consejo Superior de Investigaciones Científicas (CSIC). Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja. Instituto de Ciencias de la Vid y del Vino; España Fil: Peña Neira, Álvaro. Universidad de Chile. Facultad de Ciencias Agronómicas. Departamento de Agroindustria y Enologı́a; Chile Fil: Lijavetzky, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2018-05-24T14:06:12Z 2018-05-24T14:06:12Z 2014-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://pubs.acs.org/doi/abs/10.1021/jf501575m http://hdl.handle.net/20.500.12123/2480 0021-8561 1520-5118 http://dx.doi.org/10.1021/jf501575m eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of agricuitural and food chemistry 62 (28) : 6716–6725. (2014) |
| spellingShingle | Vid Variedades Clones Expresión Génica Antocianinas Grapevines Varieties Gene Expression Anthocyanins Malbec Muñoz, Claudio Javier Gomez Talquenca, Gonzalo Chialva, Constanza Soledad Ibáñez, Javier Martínez-Zapater, José M. Peña Neira, Alvaro Lijavetzky, Diego Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones |
| title | Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones |
| title_full | Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones |
| title_fullStr | Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones |
| title_full_unstemmed | Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones |
| title_short | Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones |
| title_sort | relationships among gene expression and anthocyanin composition of malbec grapevine clones |
| topic | Vid Variedades Clones Expresión Génica Antocianinas Grapevines Varieties Gene Expression Anthocyanins Malbec |
| url | https://pubs.acs.org/doi/abs/10.1021/jf501575m http://hdl.handle.net/20.500.12123/2480 http://dx.doi.org/10.1021/jf501575m |
| work_keys_str_mv | AT munozclaudiojavier relationshipsamonggeneexpressionandanthocyanincompositionofmalbecgrapevineclones AT gomeztalquencagonzalo relationshipsamonggeneexpressionandanthocyanincompositionofmalbecgrapevineclones AT chialvaconstanzasoledad relationshipsamonggeneexpressionandanthocyanincompositionofmalbecgrapevineclones AT ibanezjavier relationshipsamonggeneexpressionandanthocyanincompositionofmalbecgrapevineclones AT martinezzapaterjosem relationshipsamonggeneexpressionandanthocyanincompositionofmalbecgrapevineclones AT penaneiraalvaro relationshipsamonggeneexpressionandanthocyanincompositionofmalbecgrapevineclones AT lijavetzkydiego relationshipsamonggeneexpressionandanthocyanincompositionofmalbecgrapevineclones |