Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
Norovirus (NoV) is a leading cause of acute gastroenteritis (AGE) in young children worldwide. Following the introduction of universal rotavirus (RVA) vaccination in Argentina in 2015, the role of NoV in pediatric AGE warrants evaluation. This study aimed to assess the prevalence, clinical character...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/24775 https://www.mdpi.com/2076-393X/13/11/1080 https://doi.org/10.3390/vaccines13111080 |
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| author | Gomes, Karina A. Rivero, Karina A. Barrios Mathieur, Christian Degiuseppe, Juan I. Cortes, Paulo R. Gonzalez, Patricia A. Zurschmitten, Abel Castro, María P. Parreño, Gladys Viviana Mozgovoj, Marina Valeria Stupka, Juan A. |
| author_browse | Barrios Mathieur, Christian Castro, María P. Cortes, Paulo R. Degiuseppe, Juan I. Gomes, Karina A. Gonzalez, Patricia A. Mozgovoj, Marina Valeria Parreño, Gladys Viviana Rivero, Karina A. Stupka, Juan A. Zurschmitten, Abel |
| author_facet | Gomes, Karina A. Rivero, Karina A. Barrios Mathieur, Christian Degiuseppe, Juan I. Cortes, Paulo R. Gonzalez, Patricia A. Zurschmitten, Abel Castro, María P. Parreño, Gladys Viviana Mozgovoj, Marina Valeria Stupka, Juan A. |
| author_sort | Gomes, Karina A. |
| collection | INTA Digital |
| description | Norovirus (NoV) is a leading cause of acute gastroenteritis (AGE) in young children worldwide. Following the introduction of universal rotavirus (RVA) vaccination in Argentina in 2015, the role of NoV in pediatric AGE warrants evaluation. This study aimed to assess the prevalence, clinical characteristics, and molecular diversity of NoV in children under five years of age, comparing the periods before and after RVA vaccine implementation. Methods: A descriptive observational study was conducted in two pediatric hospitals in Argentina. Stool samples were obtained from both outpatient and hospitalized children presenting with acute gastroenteritis (AGE) during two distinct one-year periods: 285 samples from the pre-vaccination period (2011–2012) and 212 samples from the post-vaccination period (2019–2020). NoV, RVA and other viral enteropathogens were detected by RT-qPCR or immunoassay. Positive NoV samples were genotyped by Sanger sequencing of the ORF1/ORF2 junction. Results: NoV was detected in 30.1% (86/285) and 23.5% (50/212) of cases in the pre- and post-vaccination periods, respectively. Children under two years of age and inpatients had significantly higher NoV detection in both periods. NoV mono-infections were more frequent in post-vaccination period (72% vs. 50%). NoV GII predominated in both periods, with increased genotype diversity observed post-vaccination, including GII.3[P12], GII.4 Sydney[P16], GII.6[P7], and GII.2[P16]. Conclusions: NoV remains a major cause of pediatric AGE in Argentina, particularly in children under two years old. Although NoV prevalence did not increase after RVA vaccine introduction, its clinical relevance persists. Continued molecular surveillance is essential to monitor genotype dynamics and implement prevention strategies. |
| format | Artículo |
| id | INTA24775 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA247752025-12-26T16:52:11Z Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination Gomes, Karina A. Rivero, Karina A. Barrios Mathieur, Christian Degiuseppe, Juan I. Cortes, Paulo R. Gonzalez, Patricia A. Zurschmitten, Abel Castro, María P. Parreño, Gladys Viviana Mozgovoj, Marina Valeria Stupka, Juan A. Norovirus Viruses Children Gastroenteritis Vaccination Virus Niños Vacunación Argentina Norovirus (NoV) is a leading cause of acute gastroenteritis (AGE) in young children worldwide. Following the introduction of universal rotavirus (RVA) vaccination in Argentina in 2015, the role of NoV in pediatric AGE warrants evaluation. This study aimed to assess the prevalence, clinical characteristics, and molecular diversity of NoV in children under five years of age, comparing the periods before and after RVA vaccine implementation. Methods: A descriptive observational study was conducted in two pediatric hospitals in Argentina. Stool samples were obtained from both outpatient and hospitalized children presenting with acute gastroenteritis (AGE) during two distinct one-year periods: 285 samples from the pre-vaccination period (2011–2012) and 212 samples from the post-vaccination period (2019–2020). NoV, RVA and other viral enteropathogens were detected by RT-qPCR or immunoassay. Positive NoV samples were genotyped by Sanger sequencing of the ORF1/ORF2 junction. Results: NoV was detected in 30.1% (86/285) and 23.5% (50/212) of cases in the pre- and post-vaccination periods, respectively. Children under two years of age and inpatients had significantly higher NoV detection in both periods. NoV mono-infections were more frequent in post-vaccination period (72% vs. 50%). NoV GII predominated in both periods, with increased genotype diversity observed post-vaccination, including GII.3[P12], GII.4 Sydney[P16], GII.6[P7], and GII.2[P16]. Conclusions: NoV remains a major cause of pediatric AGE in Argentina, particularly in children under two years old. Although NoV prevalence did not increase after RVA vaccine introduction, its clinical relevance persists. Continued molecular surveillance is essential to monitor genotype dynamics and implement prevention strategies. Instituto de Virología Fil: Gomes, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina Fil: Rivero, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina Fil: Barrios Mathieur, Christian. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina Fil: Degiuseppe, Juan I. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina Fil: Cortes, Paulo R. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; Argentina Fil: Gonzalez, Patricia A. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; Argentina Fil: Zurschmitten, Abel. Hospital “Junín de los Andes” Hospital. Laboratory Section; Argentina Fil: Castro, María P. Hospital “Junín de los Andes” Hospital. Laboratory Section; Argentina Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina. Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Fil: Mozgovoj, Marina Valeria. Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS) UEDD INTA-CONICET; Argentina. 2025-12-26T16:48:23Z 2025-12-26T16:48:23Z 2025-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24775 https://www.mdpi.com/2076-393X/13/11/1080 2076-393X https://doi.org/10.3390/vaccines13111080 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Vaccines 13 (11) : 1080. (November 2025) |
| spellingShingle | Norovirus Viruses Children Gastroenteritis Vaccination Virus Niños Vacunación Argentina Gomes, Karina A. Rivero, Karina A. Barrios Mathieur, Christian Degiuseppe, Juan I. Cortes, Paulo R. Gonzalez, Patricia A. Zurschmitten, Abel Castro, María P. Parreño, Gladys Viviana Mozgovoj, Marina Valeria Stupka, Juan A. Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination |
| title | Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination |
| title_full | Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination |
| title_fullStr | Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination |
| title_full_unstemmed | Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination |
| title_short | Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination |
| title_sort | norovirus in pediatric gastroenteritis a study in argentine hospitals before and after the introduction of universal rotavirus vaccination |
| topic | Norovirus Viruses Children Gastroenteritis Vaccination Virus Niños Vacunación Argentina |
| url | http://hdl.handle.net/20.500.12123/24775 https://www.mdpi.com/2076-393X/13/11/1080 https://doi.org/10.3390/vaccines13111080 |
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