Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum
We have analysed the effects of combined auxin and cytokinin treatments on whole plant growth and leaf development in Epipremnum aureum and compared both morphological and physiological variables to those obtained with a single hormone application. Rooted cuttings of E. aureum were sprayed with 0, 5...
| Main Authors: | , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/abs/10.1080/14620316.2015.11668727 http://hdl.handle.net/20.500.12123/2469 https://doi.org/10.1080/14620316.2015.11668727 |
| _version_ | 1855034969550749696 |
|---|---|
| author | Di Benedetto, Adalberto Galmarini, Claudio Romulo Tognetti, Jorge |
| author_browse | Di Benedetto, Adalberto Galmarini, Claudio Romulo Tognetti, Jorge |
| author_facet | Di Benedetto, Adalberto Galmarini, Claudio Romulo Tognetti, Jorge |
| author_sort | Di Benedetto, Adalberto |
| collection | INTA Digital |
| description | We have analysed the effects of combined auxin and cytokinin treatments on whole plant growth and leaf development in Epipremnum aureum and compared both morphological and physiological variables to those obtained with a single hormone application. Rooted cuttings of E. aureum were sprayed with 0, 5, 50, or 100 mg I−1 indole-3- acetic acid (IAA) 7 d after transplanting. One week later, they were then sprayed with 0, 5, 50, or 100 mg I−1 benzylaminopurine (BAP). Whole plant growth, leaf development, carbon fixation, and leaf anatomy were recorded for 6 months after these sequential treatments. Following a single application of IAA or BAP, we observed an increase in the accumulation of whole-plant biomass, which reached a plateau at the highest concentration of either plant hormone. The promotion of growth was associated with increased rates of net C-assimilation and net photosynthesis, as well as with increased leaf thickness and the relative proportion of intracellular spaces in the mesophyll layer. The effect on leaves of applying both hormones, in different combinations and concentrations, resembled the results on whole plants obtained by spraying either the auxin or the cytokinin (at 50 or 100 mg I−1 BAP). Similarities in plant and leaf responses to the auxin and/or the cytokinin suggest that both hormones may act via the same pathway, which agreed with the well-known promotional effect of auxins on the development of new lateral roots, as root apices are the main site of cytokinin biosynthesis. Conversely, our results did not support the occurrence of a significant auxin-driven inhibition of cytokinin synthesis in root apices, at least for E. aureum, as reported in other species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2469 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA24692019-06-12T12:08:59Z Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum Di Benedetto, Adalberto Galmarini, Claudio Romulo Tognetti, Jorge Epipremnum Auxinas Citoquininas Sustancias de Crecimiento Vegetal Superficie Foliar Auxins Cytokinins Plant Growth Substances Leaf Area Epipremnum aureum We have analysed the effects of combined auxin and cytokinin treatments on whole plant growth and leaf development in Epipremnum aureum and compared both morphological and physiological variables to those obtained with a single hormone application. Rooted cuttings of E. aureum were sprayed with 0, 5, 50, or 100 mg I−1 indole-3- acetic acid (IAA) 7 d after transplanting. One week later, they were then sprayed with 0, 5, 50, or 100 mg I−1 benzylaminopurine (BAP). Whole plant growth, leaf development, carbon fixation, and leaf anatomy were recorded for 6 months after these sequential treatments. Following a single application of IAA or BAP, we observed an increase in the accumulation of whole-plant biomass, which reached a plateau at the highest concentration of either plant hormone. The promotion of growth was associated with increased rates of net C-assimilation and net photosynthesis, as well as with increased leaf thickness and the relative proportion of intracellular spaces in the mesophyll layer. The effect on leaves of applying both hormones, in different combinations and concentrations, resembled the results on whole plants obtained by spraying either the auxin or the cytokinin (at 50 or 100 mg I−1 BAP). Similarities in plant and leaf responses to the auxin and/or the cytokinin suggest that both hormones may act via the same pathway, which agreed with the well-known promotional effect of auxins on the development of new lateral roots, as root apices are the main site of cytokinin biosynthesis. Conversely, our results did not support the occurrence of a significant auxin-driven inhibition of cytokinin synthesis in root apices, at least for E. aureum, as reported in other species. EEA La Consulta Fil: Di Benedetto, Adalberto. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Galmarini, Claudio Romulo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina Fil: Tognetti, Jorge. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; Argentina 2018-05-23T16:02:48Z 2018-05-23T16:02:48Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tandfonline.com/doi/abs/10.1080/14620316.2015.11668727 http://hdl.handle.net/20.500.12123/2469 1462-0316 2380-4084 https://doi.org/10.1080/14620316.2015.11668727 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of horticultural science and biotechnology 90 (6) : 643-654. (2015) |
| spellingShingle | Epipremnum Auxinas Citoquininas Sustancias de Crecimiento Vegetal Superficie Foliar Auxins Cytokinins Plant Growth Substances Leaf Area Epipremnum aureum Di Benedetto, Adalberto Galmarini, Claudio Romulo Tognetti, Jorge Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum |
| title | Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum |
| title_full | Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum |
| title_fullStr | Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum |
| title_full_unstemmed | Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum |
| title_short | Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum |
| title_sort | effects of combined or single exogenous auxin and or cytokinin applications on growth and leaf area development in epipremnum aureum |
| topic | Epipremnum Auxinas Citoquininas Sustancias de Crecimiento Vegetal Superficie Foliar Auxins Cytokinins Plant Growth Substances Leaf Area Epipremnum aureum |
| url | https://www.tandfonline.com/doi/abs/10.1080/14620316.2015.11668727 http://hdl.handle.net/20.500.12123/2469 https://doi.org/10.1080/14620316.2015.11668727 |
| work_keys_str_mv | AT dibenedettoadalberto effectsofcombinedorsingleexogenousauxinandorcytokininapplicationsongrowthandleafareadevelopmentinepipremnumaureum AT galmariniclaudioromulo effectsofcombinedorsingleexogenousauxinandorcytokininapplicationsongrowthandleafareadevelopmentinepipremnumaureum AT tognettijorge effectsofcombinedorsingleexogenousauxinandorcytokininapplicationsongrowthandleafareadevelopmentinepipremnumaureum |