Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/product...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://link.springer.com/article/10.1007/s11557-015-1147-7 http://hdl.handle.net/20.500.12123/2454 https://doi.org/10.1007/s11557-015-1147-7 |
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| author | Ortega, Leonel Maximilano Dinolfo, María Inés Astoreca, Andrea Luciana Alberione, Enrique Javier Stenglein, Sebastian Alberto Alconada Magliano, Teresa Maria |
| author_browse | Alberione, Enrique Javier Alconada Magliano, Teresa Maria Astoreca, Andrea Luciana Dinolfo, María Inés Ortega, Leonel Maximilano Stenglein, Sebastian Alberto |
| author_facet | Ortega, Leonel Maximilano Dinolfo, María Inés Astoreca, Andrea Luciana Alberione, Enrique Javier Stenglein, Sebastian Alberto Alconada Magliano, Teresa Maria |
| author_sort | Ortega, Leonel Maximilano |
| collection | INTA Digital |
| description | Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area. |
| format | Artículo |
| id | INTA2454 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA24542018-06-21T13:57:40Z Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina Ortega, Leonel Maximilano Dinolfo, María Inés Astoreca, Andrea Luciana Alberione, Enrique Javier Stenglein, Sebastian Alberto Alconada Magliano, Teresa Maria Enfermedades de las Plantas Gibberella Zeae Trigo Fusarium Fusariosis Marcadores Genéticos Plant Diseases Wheat Genetic Markers Fusarium Graminearum Argentina Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area. EEA Marcos Juárez Fil: Ortega, Leonel Maximilano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Dinolfo, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina Fil: Astoreca, Andrea Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Alberione, Enrique Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología; Argentina Fil: Alconada Magliano, Teresa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina 2018-05-22T15:09:40Z 2018-05-22T15:09:40Z 2016-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s11557-015-1147-7 http://hdl.handle.net/20.500.12123/2454 1617-416X 1861-8952 https://doi.org/10.1007/s11557-015-1147-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Mycological progress 15 : 1. (January 2016) |
| spellingShingle | Enfermedades de las Plantas Gibberella Zeae Trigo Fusarium Fusariosis Marcadores Genéticos Plant Diseases Wheat Genetic Markers Fusarium Graminearum Argentina Ortega, Leonel Maximilano Dinolfo, María Inés Astoreca, Andrea Luciana Alberione, Enrique Javier Stenglein, Sebastian Alberto Alconada Magliano, Teresa Maria Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina |
| title | Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina |
| title_full | Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina |
| title_fullStr | Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina |
| title_full_unstemmed | Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina |
| title_short | Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina |
| title_sort | molecular and mycotoxin characterization of fusarium graminearum isolates obtained from wheat at a single field in argentina |
| topic | Enfermedades de las Plantas Gibberella Zeae Trigo Fusarium Fusariosis Marcadores Genéticos Plant Diseases Wheat Genetic Markers Fusarium Graminearum Argentina |
| url | https://link.springer.com/article/10.1007/s11557-015-1147-7 http://hdl.handle.net/20.500.12123/2454 https://doi.org/10.1007/s11557-015-1147-7 |
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