An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint
Fusarium head blight (FHB) caused by species within the Fusarium graminearum complex is a devastating disease that causes extensive yield and quality losses to wheat in humid and semi-humid regions of the world. The disease can cause not only yield losses but also grain contamination with mycotoxins...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S2352407316300312 http://hdl.handle.net/20.500.12123/2453 https://doi.org/10.1016/j.plgene.2017.07.002 |
| _version_ | 1855483058888638464 |
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| author | Palazzini, Juan Manuel Yerkovich, Nadia Alberione, Enrique Javier Chiotta, María Laura Chulze, Sofia Noemí |
| author_browse | Alberione, Enrique Javier Chiotta, María Laura Chulze, Sofia Noemí Palazzini, Juan Manuel Yerkovich, Nadia |
| author_facet | Palazzini, Juan Manuel Yerkovich, Nadia Alberione, Enrique Javier Chiotta, María Laura Chulze, Sofia Noemí |
| author_sort | Palazzini, Juan Manuel |
| collection | INTA Digital |
| description | Fusarium head blight (FHB) caused by species within the Fusarium graminearum complex is a devastating disease that causes extensive yield and quality losses to wheat in humid and semi-humid regions of the world. The disease can cause not only yield losses but also grain contamination with mycotoxins such as deoxynivalenol (DON). Several epidemics have occurred in Argentina, the last in 2012, with yield losses up to 70%. Control of the disease is partially achieved by chemical treatments, crop rotation, breeding for resistance and tillage practices. Biocontrol can be used as part of an integrated pest management. The objetives of this study were: 1- to evaluate the biological control effect of Streptomyces sp. RC 87B on FHB disease incidence, severity and DON accumulation and 2- to evaluate if Streptomyces sp. RC 87B reduces F. graminearum inoculum when applied to wheat stubble. Streptomyces sp. RC 87B was applied at wheat anthesis to determine effects on FHB disease parameters and over wheat stubble left after harvest to evaluate effects on the survival of F. graminearum. FHB incidence and severity were visually estimated and DON content was assessed by HPLC after harvest. On stubble, F. graminearum prevalence was monitored by using TaqMan PCR for 180 days. A significant disease severity and DON reduction were observed with the biocontrol treatments during the field trials (29–39% and 69–85%, respectively), but no reduction on disease incidence was achieved. On wheat stubble, Streptomyces sp. 87B reduced F. graminearum inoculum after 45 days (82%) and after 90 days no inoculum was detected in the first of two trials. In a second trial, a significant reduction (46%) in F. graminearum inoculum was observed after 90 days. The biocontrol strain Streptomyces sp. 87B showed high potential for contributing to the reduction of FHB on wheat at different times in the F. graminearum life cycle. |
| format | Artículo |
| id | INTA2453 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA24532019-10-01T17:07:19Z An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint Palazzini, Juan Manuel Yerkovich, Nadia Alberione, Enrique Javier Chiotta, María Laura Chulze, Sofia Noemí Enfermedades de las Plantas Fusarium Fusariosis Control Biológico Trigo Streptomyces Plant Diseases Biological Control Wheat Gibberella Zeae Fusarium Graminearum Fusarium head blight (FHB) caused by species within the Fusarium graminearum complex is a devastating disease that causes extensive yield and quality losses to wheat in humid and semi-humid regions of the world. The disease can cause not only yield losses but also grain contamination with mycotoxins such as deoxynivalenol (DON). Several epidemics have occurred in Argentina, the last in 2012, with yield losses up to 70%. Control of the disease is partially achieved by chemical treatments, crop rotation, breeding for resistance and tillage practices. Biocontrol can be used as part of an integrated pest management. The objetives of this study were: 1- to evaluate the biological control effect of Streptomyces sp. RC 87B on FHB disease incidence, severity and DON accumulation and 2- to evaluate if Streptomyces sp. RC 87B reduces F. graminearum inoculum when applied to wheat stubble. Streptomyces sp. RC 87B was applied at wheat anthesis to determine effects on FHB disease parameters and over wheat stubble left after harvest to evaluate effects on the survival of F. graminearum. FHB incidence and severity were visually estimated and DON content was assessed by HPLC after harvest. On stubble, F. graminearum prevalence was monitored by using TaqMan PCR for 180 days. A significant disease severity and DON reduction were observed with the biocontrol treatments during the field trials (29–39% and 69–85%, respectively), but no reduction on disease incidence was achieved. On wheat stubble, Streptomyces sp. 87B reduced F. graminearum inoculum after 45 days (82%) and after 90 days no inoculum was detected in the first of two trials. In a second trial, a significant reduction (46%) in F. graminearum inoculum was observed after 90 days. The biocontrol strain Streptomyces sp. 87B showed high potential for contributing to the reduction of FHB on wheat at different times in the F. graminearum life cycle. EEA Marcos Juárez Fil: Palazzini, Juan Manuel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Yerkovich, N. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Alberione, Enrique Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Chiotta, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Chulze, Sofia Noemí. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico, Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina 2018-05-22T14:39:22Z 2018-05-22T14:39:22Z 2017-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S2352407316300312 http://hdl.handle.net/20.500.12123/2453 2352-4073 https://doi.org/10.1016/j.plgene.2017.07.002 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plant gene 11 (Part A) : 2-7. (September 2017) |
| spellingShingle | Enfermedades de las Plantas Fusarium Fusariosis Control Biológico Trigo Streptomyces Plant Diseases Biological Control Wheat Gibberella Zeae Fusarium Graminearum Palazzini, Juan Manuel Yerkovich, Nadia Alberione, Enrique Javier Chiotta, María Laura Chulze, Sofia Noemí An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint |
| title | An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint |
| title_full | An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint |
| title_fullStr | An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint |
| title_full_unstemmed | An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint |
| title_short | An integrated dual strategy to control Fusarium graminearum sensu stricto by the biocontrol agent Streptomyces sp. RC 87B under field conditions. Reprint |
| title_sort | integrated dual strategy to control fusarium graminearum sensu stricto by the biocontrol agent streptomyces sp rc 87b under field conditions reprint |
| topic | Enfermedades de las Plantas Fusarium Fusariosis Control Biológico Trigo Streptomyces Plant Diseases Biological Control Wheat Gibberella Zeae Fusarium Graminearum |
| url | https://www.sciencedirect.com/science/article/pii/S2352407316300312 http://hdl.handle.net/20.500.12123/2453 https://doi.org/10.1016/j.plgene.2017.07.002 |
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