Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
The study of hypothyroidism and experimentally induced goitre in young goats enables the characterization of hormonal dynamics and histological alterations in the thyroid gland. The objective of this study was to characterize histopathological and histomorphometric changes in the thyroid glands (TG)...
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/24523 https://www.sciencedirect.com/science/article/abs/pii/S003452882500400X https://doi.org/10.1016/j.rvsc.2025.105926 |
| _version_ | 1855487342270218240 |
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| author | Colque Caro, Luis Adrián Barbeito, Claudio Gustavo Moore, Prando Dadin Micheloud, Juan Francisco |
| author_browse | Barbeito, Claudio Gustavo Colque Caro, Luis Adrián Micheloud, Juan Francisco Moore, Prando Dadin |
| author_facet | Colque Caro, Luis Adrián Barbeito, Claudio Gustavo Moore, Prando Dadin Micheloud, Juan Francisco |
| author_sort | Colque Caro, Luis Adrián |
| collection | INTA Digital |
| description | The study of hypothyroidism and experimentally induced goitre in young goats enables the characterization of hormonal dynamics and histological alterations in the thyroid gland. The objective of this study was to characterize histopathological and histomorphometric changes in the thyroid glands (TG) of goat kids following experimental induction of goitre using 6-n-propyl-2-thiouracil (PTU). Eighteen healthy lactating Saanen goat kids (35 days old; 6.2 kg body weight) were randomly assigned to three groups: control (basal diet +3 ml fresh milk), low-dose PTU (3.5 mg/kg BW/day in 3 ml milk), and high-dose PTU (7 mg/kg BW/day in 3 ml milk). The study spanned 116 days, during which participants underwent weekly body weight monitoring and PTU dose adjustments. At the conclusion of the trial, necropsies were conducted on 16 animals, and their TGs were evaluated both macroscopically and microscopically. Blood samples were collected on a biweekly basis to assess circulating thyroid hormone levels. No significant differences in weight gain were observed during the first 56 days; however, from day 56 onward, children in the high-dose PTU group exhibited reduced average daily gain compared to the low-dose and control groups. Thyroid gland weights exhibited an increase in both treated groups (up to 10.5 μg), accounting for 0.04 % and 0.03 % of body weight in the low- and high-dose groups, respectively, compared to 0.01 % in the control group. A histopathological evaluation revealed marked follicular hyperplasia and hypertrophy, with significant alterations in follicular morphology, epithelial height, and interfollicular spacing. Hormonal profiles revealed dose-dependent disruptions in T3, T4, and T3/T4 ratios, with the most pronounced alterations observed in high-dose PTU-treated animals. Chronic administration of PTU (propylthiouracil) led to significant structural and functional alterations in the thyroid gland, validating the experimental model's suitability for investigating iodine deficiency–related goitre in young ruminants. |
| format | Artículo |
| id | INTA24523 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA245232025-11-10T13:26:14Z Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings Colque Caro, Luis Adrián Barbeito, Claudio Gustavo Moore, Prando Dadin Micheloud, Juan Francisco Caprinos Enfermedades de los Animales Hipotiroidismo Bocio Histomorfología Goats Animal Diseases Hypothyroidism Goitre Histomorphology The study of hypothyroidism and experimentally induced goitre in young goats enables the characterization of hormonal dynamics and histological alterations in the thyroid gland. The objective of this study was to characterize histopathological and histomorphometric changes in the thyroid glands (TG) of goat kids following experimental induction of goitre using 6-n-propyl-2-thiouracil (PTU). Eighteen healthy lactating Saanen goat kids (35 days old; 6.2 kg body weight) were randomly assigned to three groups: control (basal diet +3 ml fresh milk), low-dose PTU (3.5 mg/kg BW/day in 3 ml milk), and high-dose PTU (7 mg/kg BW/day in 3 ml milk). The study spanned 116 days, during which participants underwent weekly body weight monitoring and PTU dose adjustments. At the conclusion of the trial, necropsies were conducted on 16 animals, and their TGs were evaluated both macroscopically and microscopically. Blood samples were collected on a biweekly basis to assess circulating thyroid hormone levels. No significant differences in weight gain were observed during the first 56 days; however, from day 56 onward, children in the high-dose PTU group exhibited reduced average daily gain compared to the low-dose and control groups. Thyroid gland weights exhibited an increase in both treated groups (up to 10.5 μg), accounting for 0.04 % and 0.03 % of body weight in the low- and high-dose groups, respectively, compared to 0.01 % in the control group. A histopathological evaluation revealed marked follicular hyperplasia and hypertrophy, with significant alterations in follicular morphology, epithelial height, and interfollicular spacing. Hormonal profiles revealed dose-dependent disruptions in T3, T4, and T3/T4 ratios, with the most pronounced alterations observed in high-dose PTU-treated animals. Chronic administration of PTU (propylthiouracil) led to significant structural and functional alterations in the thyroid gland, validating the experimental model's suitability for investigating iodine deficiency–related goitre in young ruminants. Instituto de Investigación Animal del Chaco Semiárido Fil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina Fil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; Argentina Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Experimental y Comparada. Laboratorio de Histología y Embriología Descriptiva; Argentina Fil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT La Plata; Argentina Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Moore, Prando Dadin. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; Argentina Fil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina 2025-11-10T13:22:59Z 2025-11-10T13:22:59Z 2025-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24523 https://www.sciencedirect.com/science/article/abs/pii/S003452882500400X 1532-2661 0034-5288 https://doi.org/10.1016/j.rvsc.2025.105926 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Research in Veterinary Science 197 : 105926. (December 2025) |
| spellingShingle | Caprinos Enfermedades de los Animales Hipotiroidismo Bocio Histomorfología Goats Animal Diseases Hypothyroidism Goitre Histomorphology Colque Caro, Luis Adrián Barbeito, Claudio Gustavo Moore, Prando Dadin Micheloud, Juan Francisco Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings |
| title | Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings |
| title_full | Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings |
| title_fullStr | Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings |
| title_full_unstemmed | Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings |
| title_short | Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings |
| title_sort | experimental induction of hypothyroidism and goitre in goat kids clinical pathological and histomorphometric findings |
| topic | Caprinos Enfermedades de los Animales Hipotiroidismo Bocio Histomorfología Goats Animal Diseases Hypothyroidism Goitre Histomorphology |
| url | http://hdl.handle.net/20.500.12123/24523 https://www.sciencedirect.com/science/article/abs/pii/S003452882500400X https://doi.org/10.1016/j.rvsc.2025.105926 |
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