Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
Agriculture in the Argentine Puna faces significant challenges due to adverse climatic conditions and poor sandy soils prone to erosion and nutrient loss, particularly nitrogen (N), which is essential for plant growth. Despite these challenges, there is limited information on the role of beneficial...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24522 https://www.sciencedirect.com/science/article/abs/pii/S0929139325006808 https://doi.org/10.1016/j.apsoil.2025.106542 |
| _version_ | 1855038956292276224 |
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| author | Hernandez Guijarro, Keren Covacevich, Fernanda Caruso, Graciela Castro, Dario Carlos Feingold, Sergio Enrique |
| author_browse | Caruso, Graciela Castro, Dario Carlos Covacevich, Fernanda Feingold, Sergio Enrique Hernandez Guijarro, Keren |
| author_facet | Hernandez Guijarro, Keren Covacevich, Fernanda Caruso, Graciela Castro, Dario Carlos Feingold, Sergio Enrique |
| author_sort | Hernandez Guijarro, Keren |
| collection | INTA Digital |
| description | Agriculture in the Argentine Puna faces significant challenges due to adverse climatic conditions and poor sandy soils prone to erosion and nutrient loss, particularly nitrogen (N), which is essential for plant growth. Despite these challenges, there is limited information on the role of beneficial soil microorganisms in nutrient cycling and uptake in this extreme environment. This study aimed to investigate the impact of agricultural practices on N-cycling microorganisms and arbuscular mycorrhizal fungi (AMF) interactions in Abra Pampa soils, located 3487 m above sea level. We conducted three annual samplings during the summers of 2020, 2021, and 2022 across four treatments: two agricultural plots (A1 and A2) amended with llama manure, each under different crop rotations - A1: Andean potato-quinoa-mallow and A2: quinoa-triticale-mallow; one soil under decomposing llama manure (BG) adjacent to agricultural plots; and a pristine control soil covered with native vegetation. We quantified the N-cycling genes (nifH, amoA from Archaea (AOA) and Bacteria (AOB), nirK, nirS and nosZ), and assessed AMF functional activity (percentage of colonization, arbuscule formation and number of spores in soil) and AMF diversity (Glomeraceae family) using PCR-SSCP analysis. Climate conditions were found to significantly influence the abundance of denitrification genes (nirK and nosZ) and AMF functional parameters, correlating with soil ammonia and nitrate concentrations. Variations in nifH abundance and the AOA/AOB ratio were associated with agricultural practices and soil parameters such as ammonia and available phosphorus content, respectively. Notably, associations between N-cycling bacteria and AMF functions suggest synergistic rather than antagonistic interactions between these groups. Agricultural activities were found to adversely affect AMF genetic diversity. The BG site emerged as a hotspot with a significant abundance of denitrification genes, warranting further investigation in future studies. This study provides valuable insights into the potential interactions among microorganisms involved in N cycling and AMF under various land use scenarios in the Argentine Puna, highlighting considerations for developing sustainable agricultural strategies in the region. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24522 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA245222025-11-10T12:59:00Z Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils Hernandez Guijarro, Keren Covacevich, Fernanda Caruso, Graciela Castro, Dario Carlos Feingold, Sergio Enrique Agricultura Prácticas Agrícolas Suelo Nitrógeno Utilización de la Tierra Agriculture Agricultural Practices Soil Nitrogen Mycorrhizae Land Use Región Puna Argentina Agriculture in the Argentine Puna faces significant challenges due to adverse climatic conditions and poor sandy soils prone to erosion and nutrient loss, particularly nitrogen (N), which is essential for plant growth. Despite these challenges, there is limited information on the role of beneficial soil microorganisms in nutrient cycling and uptake in this extreme environment. This study aimed to investigate the impact of agricultural practices on N-cycling microorganisms and arbuscular mycorrhizal fungi (AMF) interactions in Abra Pampa soils, located 3487 m above sea level. We conducted three annual samplings during the summers of 2020, 2021, and 2022 across four treatments: two agricultural plots (A1 and A2) amended with llama manure, each under different crop rotations - A1: Andean potato-quinoa-mallow and A2: quinoa-triticale-mallow; one soil under decomposing llama manure (BG) adjacent to agricultural plots; and a pristine control soil covered with native vegetation. We quantified the N-cycling genes (nifH, amoA from Archaea (AOA) and Bacteria (AOB), nirK, nirS and nosZ), and assessed AMF functional activity (percentage of colonization, arbuscule formation and number of spores in soil) and AMF diversity (Glomeraceae family) using PCR-SSCP analysis. Climate conditions were found to significantly influence the abundance of denitrification genes (nirK and nosZ) and AMF functional parameters, correlating with soil ammonia and nitrate concentrations. Variations in nifH abundance and the AOA/AOB ratio were associated with agricultural practices and soil parameters such as ammonia and available phosphorus content, respectively. Notably, associations between N-cycling bacteria and AMF functions suggest synergistic rather than antagonistic interactions between these groups. Agricultural activities were found to adversely affect AMF genetic diversity. The BG site emerged as a hotspot with a significant abundance of denitrification genes, warranting further investigation in future studies. This study provides valuable insights into the potential interactions among microorganisms involved in N cycling and AMF under various land use scenarios in the Argentine Puna, highlighting considerations for developing sustainable agricultural strategies in the region. EEA Balcarce Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Hernández Guijarro, Keren. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Covacevich, Fernanada. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fil: Caruso, Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Castro, Dario Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina 2025-11-10T12:56:18Z 2025-11-10T12:56:18Z 2025-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24522 https://www.sciencedirect.com/science/article/abs/pii/S0929139325006808 0929-1393 1873-0272 https://doi.org/10.1016/j.apsoil.2025.106542 eng info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo info:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradación info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Applied Soil Ecology 216 : 106542. (December 2025) |
| spellingShingle | Agricultura Prácticas Agrícolas Suelo Nitrógeno Utilización de la Tierra Agriculture Agricultural Practices Soil Nitrogen Mycorrhizae Land Use Región Puna Argentina Hernandez Guijarro, Keren Covacevich, Fernanda Caruso, Graciela Castro, Dario Carlos Feingold, Sergio Enrique Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils |
| title | Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils |
| title_full | Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils |
| title_fullStr | Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils |
| title_full_unstemmed | Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils |
| title_short | Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils |
| title_sort | influence of agricultural practices on nitrogen cycling microbial communities and their interaction with mycorrhizae in argentine puna soils |
| topic | Agricultura Prácticas Agrícolas Suelo Nitrógeno Utilización de la Tierra Agriculture Agricultural Practices Soil Nitrogen Mycorrhizae Land Use Región Puna Argentina |
| url | http://hdl.handle.net/20.500.12123/24522 https://www.sciencedirect.com/science/article/abs/pii/S0929139325006808 https://doi.org/10.1016/j.apsoil.2025.106542 |
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