Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system
The first objective of this study was to identify and characterize new strains isolated from rice–pasture rotation soils, capable of: (A) establishing efficient symbiosis with Lotus plants and (B) enhancing their performance under N-deficient soil conditions, outperforming the Lotus reference stra...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Español |
| Publicado: |
Springer
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24519 https://link.springer.com/article/10.1007/s13199-025-01088-5 https://doi.org/10.1007/s13199-025-01088-5 |
| _version_ | 1855038955763793920 |
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| author | Maguire, Vanina Giselle Rodríguez, Andrés Alberto Alonso, Andrés Mariano Ezquiaga, Juan Pedro Salas, Nehuén Llames, María Eugenia Gortari, Maximiliano Ayub, Nicolás Daniel Brambilla, Silvina Maricel Bouilly, Pedro José Romero, Fernando Matías Gárriz, Andrés Ruíz, Oscar Adolfo |
| author_browse | Alonso, Andrés Mariano Ayub, Nicolás Daniel Bouilly, Pedro José Brambilla, Silvina Maricel Ezquiaga, Juan Pedro Gortari, Maximiliano Gárriz, Andrés Llames, María Eugenia Maguire, Vanina Giselle Rodríguez, Andrés Alberto Romero, Fernando Matías Ruíz, Oscar Adolfo Salas, Nehuén |
| author_facet | Maguire, Vanina Giselle Rodríguez, Andrés Alberto Alonso, Andrés Mariano Ezquiaga, Juan Pedro Salas, Nehuén Llames, María Eugenia Gortari, Maximiliano Ayub, Nicolás Daniel Brambilla, Silvina Maricel Bouilly, Pedro José Romero, Fernando Matías Gárriz, Andrés Ruíz, Oscar Adolfo |
| author_sort | Maguire, Vanina Giselle |
| collection | INTA Digital |
| description | The first objective of this study was to identify and characterize new strains isolated from rice–pasture rotation soils, capable of: (A) establishing efficient symbiosis with Lotus plants and (B) enhancing their performance under N-deficient soil conditions, outperforming the Lotus reference strain 700743. To test this, we used the trap plant technique to iso late native rhizobial strains from these soils. We characterized their potential through nodulation and plant performance parameters, including number of nodules, total nodule weight per plant, total leaf area, and stem parameters such as fresh weight, dry weight, and nitrogen content. These parameters were determined in plants inoculated with these strains in a nitrogen-restricted medium. As a second objective, we aimed to genetically characterize the strains previously selected and compare them with the reference strain. Two isolated strains were characterized, RIZ17 and RIZ20, both belonging to the genus Mesorhizobium, which showed plant performance similar to or greater than the reference strain 700743. In particular, RIZ17 outperformed the reference strain in nodulation and plant performance parameters of Lotus plants grown under nitrogen-deficient conditions. Genomic analysis did not reveal differences among the three strains at the gene sequence level related to nodulation and nitrogen fixation. However, it indicated differences in gene sequences linked to bacterial nutritional metabolism. These differences could provide an adaptive advantage to RIZ17 by efficiently metaboliz ing plant-derived nutrients, resulting in greater energy generation for symbiosis establishment and increased root nodule number and weight. The studied strains could be promising new rhizobia for inoculant formulation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24519 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA245192025-11-10T12:00:15Z Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system Maguire, Vanina Giselle Rodríguez, Andrés Alberto Alonso, Andrés Mariano Ezquiaga, Juan Pedro Salas, Nehuén Llames, María Eugenia Gortari, Maximiliano Ayub, Nicolás Daniel Brambilla, Silvina Maricel Bouilly, Pedro José Romero, Fernando Matías Gárriz, Andrés Ruíz, Oscar Adolfo Rotación de Cultivos Lotus Crop Rotation Rhizobacteria Rice Pastures Rizobacteria Arroz Pastizales The first objective of this study was to identify and characterize new strains isolated from rice–pasture rotation soils, capable of: (A) establishing efficient symbiosis with Lotus plants and (B) enhancing their performance under N-deficient soil conditions, outperforming the Lotus reference strain 700743. To test this, we used the trap plant technique to iso late native rhizobial strains from these soils. We characterized their potential through nodulation and plant performance parameters, including number of nodules, total nodule weight per plant, total leaf area, and stem parameters such as fresh weight, dry weight, and nitrogen content. These parameters were determined in plants inoculated with these strains in a nitrogen-restricted medium. As a second objective, we aimed to genetically characterize the strains previously selected and compare them with the reference strain. Two isolated strains were characterized, RIZ17 and RIZ20, both belonging to the genus Mesorhizobium, which showed plant performance similar to or greater than the reference strain 700743. In particular, RIZ17 outperformed the reference strain in nodulation and plant performance parameters of Lotus plants grown under nitrogen-deficient conditions. Genomic analysis did not reveal differences among the three strains at the gene sequence level related to nodulation and nitrogen fixation. However, it indicated differences in gene sequences linked to bacterial nutritional metabolism. These differences could provide an adaptive advantage to RIZ17 by efficiently metaboliz ing plant-derived nutrients, resulting in greater energy generation for symbiosis establishment and increased root nodule number and weight. The studied strains could be promising new rhizobia for inoculant formulation. EEA Manfredi Fil: Maguire, Vanina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Maguire, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Área de Mejoramiento Genético Vegetal - Bioestadística; Argentina Fil: Rodríguez, Andrés Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Fisiología y Asistencia Al Mejoramiento Vegetal; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Alonso, Andrés Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Parasitología Molecular; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Ezquiaga, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Salas, Nehuén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Parásitos Anaerobios; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Salas, Nehuén. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Llames, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Ecología y Fotobiología Acuática; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Gortari, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Ayub, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bouilly, Pedro José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Romero, Fernando Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Gárriz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina Fil: Ruíz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús. Laboratorio de Estrés Biótico y Abiótico en Plantas; Argentina. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús. Escuela de Bio y Nanotecnologías; Argentina 2025-11-10T11:38:11Z 2025-11-10T11:38:11Z 2025-11-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24519 https://link.springer.com/article/10.1007/s13199-025-01088-5 Maguire, V.G., Rodríguez, A.A., Alonso, A.M. et al. Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system. Symbiosis (2025). https://doi.org/10.1007/s13199-025-01088-5 0334-5114 1878-7665 https://doi.org/10.1007/s13199-025-01088-5 spa info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Symbiosis : 1-17 (Published online: 03 november 2025) |
| spellingShingle | Rotación de Cultivos Lotus Crop Rotation Rhizobacteria Rice Pastures Rizobacteria Arroz Pastizales Maguire, Vanina Giselle Rodríguez, Andrés Alberto Alonso, Andrés Mariano Ezquiaga, Juan Pedro Salas, Nehuén Llames, María Eugenia Gortari, Maximiliano Ayub, Nicolás Daniel Brambilla, Silvina Maricel Bouilly, Pedro José Romero, Fernando Matías Gárriz, Andrés Ruíz, Oscar Adolfo Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system |
| title | Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system |
| title_full | Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system |
| title_fullStr | Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system |
| title_full_unstemmed | Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system |
| title_short | Exploring the relationship of potential new rhizobacteria metabolism with Lotus spp. performance in a rice-pasture rotation system |
| title_sort | exploring the relationship of potential new rhizobacteria metabolism with lotus spp performance in a rice pasture rotation system |
| topic | Rotación de Cultivos Lotus Crop Rotation Rhizobacteria Rice Pastures Rizobacteria Arroz Pastizales |
| url | http://hdl.handle.net/20.500.12123/24519 https://link.springer.com/article/10.1007/s13199-025-01088-5 https://doi.org/10.1007/s13199-025-01088-5 |
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