Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods
Leptospirosis is a globally distributed zoonotic disease. This study aimed to evaluate the performance of loop-mediated isothermal amplification (LAMP) in detecting Leptospira DNA in bovine samples compared to conventional lipL32 PCR and serological methods. A total of 464 serum, 96 urine, and 31 or...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/24460 https://www.sciencedirect.com/science/article/abs/pii/S0378113525002974 https://doi.org/10.1016/j.vetmic.2025.110662 |
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| author | Hamer, Micaela Saraullo, Vanina Rosa Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_browse | Brihuega, Bibiana Felicitas Esteban, Micaela Hamer, Micaela Martinez, Mara Leila Sanchez, Maria Cristina Saraullo, Vanina Rosa |
| author_facet | Hamer, Micaela Saraullo, Vanina Rosa Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_sort | Hamer, Micaela |
| collection | INTA Digital |
| description | Leptospirosis is a globally distributed zoonotic disease. This study aimed to evaluate the performance of loop-mediated isothermal amplification (LAMP) in detecting Leptospira DNA in bovine samples compared to conventional lipL32 PCR and serological methods. A total of 464 serum, 96 urine, and 31 organ samples were analyzed using LAMP, lipL32 PCR, and the microscopic agglutination test (MAT). The results showed that 52.8 % of sera tested positive exclusively by MAT, indicating past exposure, while 1.2 % tested positive by molecular techniques, suggesting early infection. In urine samples, LAMP detected leptospiral DNA in 62.5 % of cases, highlighting its potential for identifying asymptomatic carriers. Among organ samples from aborted fetuses, 22.6 % were positive by both molecular techniques, supporting Leptospira as a potential cause of fetal loss. Notably, LAMP exhibited a high diagnostic sensitivity (100 %, 95 % CI: 93.5–100 %) and specificity (95.4 %, 95 % CI: 93.2–97.0 %), outperforming lipL32 PCR in DNA detection. Additionally, Cohen’s Kappa index (0.792) indicated substantial agreement between LAMP and lipL32 PCR. Given its higher sensitivity, rapid turnaround, and affordability, LAMP represents a valuable tool for improving leptospirosis diagnosis, particularly in resource-limited settings. These findings underscore the importance of integrating molecular and serological techniques for a more accurate detection of Leptospira infection in cattle. |
| format | Artículo |
| id | INTA24460 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA244602025-11-05T11:27:29Z Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods Hamer, Micaela Saraullo, Vanina Rosa Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila PCR Agglutination Tests Leptospirosis Cattle Isothermal Processes Immunological Techniques Leptospira Reacción de Aglutinación Ganado Bovino Proceso Isotérmico Técnicas Inmunológicas Leptospirosis is a globally distributed zoonotic disease. This study aimed to evaluate the performance of loop-mediated isothermal amplification (LAMP) in detecting Leptospira DNA in bovine samples compared to conventional lipL32 PCR and serological methods. A total of 464 serum, 96 urine, and 31 organ samples were analyzed using LAMP, lipL32 PCR, and the microscopic agglutination test (MAT). The results showed that 52.8 % of sera tested positive exclusively by MAT, indicating past exposure, while 1.2 % tested positive by molecular techniques, suggesting early infection. In urine samples, LAMP detected leptospiral DNA in 62.5 % of cases, highlighting its potential for identifying asymptomatic carriers. Among organ samples from aborted fetuses, 22.6 % were positive by both molecular techniques, supporting Leptospira as a potential cause of fetal loss. Notably, LAMP exhibited a high diagnostic sensitivity (100 %, 95 % CI: 93.5–100 %) and specificity (95.4 %, 95 % CI: 93.2–97.0 %), outperforming lipL32 PCR in DNA detection. Additionally, Cohen’s Kappa index (0.792) indicated substantial agreement between LAMP and lipL32 PCR. Given its higher sensitivity, rapid turnaround, and affordability, LAMP represents a valuable tool for improving leptospirosis diagnosis, particularly in resource-limited settings. These findings underscore the importance of integrating molecular and serological techniques for a more accurate detection of Leptospira infection in cattle. Instituto de Patobiología Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Escuela de Veterinaria; Argentina Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Universidad del Salvador. Escuela de Veterinaria; Argentina 2025-11-05T11:16:47Z 2025-11-05T11:16:47Z 2025-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24460 https://www.sciencedirect.com/science/article/abs/pii/S0378113525002974 0378-1135 https://doi.org/10.1016/j.vetmic.2025.110662 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Microbiology 308 : 110662 (September 2025) |
| spellingShingle | PCR Agglutination Tests Leptospirosis Cattle Isothermal Processes Immunological Techniques Leptospira Reacción de Aglutinación Ganado Bovino Proceso Isotérmico Técnicas Inmunológicas Hamer, Micaela Saraullo, Vanina Rosa Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods |
| title | Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods |
| title_full | Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods |
| title_fullStr | Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods |
| title_full_unstemmed | Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods |
| title_short | Enhanced detection of Leptospira in cattle : Comparative performance of loop-mediated isothermal amplification, polymerase chain reaction and serological methods |
| title_sort | enhanced detection of leptospira in cattle comparative performance of loop mediated isothermal amplification polymerase chain reaction and serological methods |
| topic | PCR Agglutination Tests Leptospirosis Cattle Isothermal Processes Immunological Techniques Leptospira Reacción de Aglutinación Ganado Bovino Proceso Isotérmico Técnicas Inmunológicas |
| url | http://hdl.handle.net/20.500.12123/24460 https://www.sciencedirect.com/science/article/abs/pii/S0378113525002974 https://doi.org/10.1016/j.vetmic.2025.110662 |
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