Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol
The increasing demand for sustainable agriculture has driven the search for biological control agents targeting plant-parasitic nematodes. This study characterised Serratia odorifera strain INTA L401-1, isolated for the first time from the hemolymph of Tenebrio molitor larvae infected with Heterorha...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Taylor & Francis
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24379 https://www.tandfonline.com/doi/10.1080/09583157.2025.2520250 https://doi.org/10.1080/09583157.2025.2520250 |
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| author | Salas, Augusto Ortiz, Leila Melina Niz, José María Magariños, Francisco Del Valle, Eleodoro Eduardo Achinelly, María Fernanda Sauka, Diego Hernan |
| author_browse | Achinelly, María Fernanda Del Valle, Eleodoro Eduardo Magariños, Francisco Niz, José María Ortiz, Leila Melina Salas, Augusto Sauka, Diego Hernan |
| author_facet | Salas, Augusto Ortiz, Leila Melina Niz, José María Magariños, Francisco Del Valle, Eleodoro Eduardo Achinelly, María Fernanda Sauka, Diego Hernan |
| author_sort | Salas, Augusto |
| collection | INTA Digital |
| description | The increasing demand for sustainable agriculture has driven the search for biological control agents targeting plant-parasitic nematodes. This study characterised Serratia odorifera strain INTA L401-1, isolated for the first time from the hemolymph of Tenebrio molitor larvae infected with Heterorhabditis bacteriophora, and evaluated its nematicidal activity. Phylogenetic analysis using 16S rRNA sequencing, along with phenotypic profiling, identified the strain as S. odorifera with 99% confidence. In vitro bioassays demonstrated significant activity against Panagrellus redivivus, with 24-hour-old cultures achieving 89.1% mortality on nutrient agar, attributed to peak secondary metabolite production. Mortality declined in 48–72-hour-old cultures but increased after seven days, likely due to metabolite accumulation. Filtered supernatants of nutrient broth cultures retained nematicidal activity (>40%), implicating secreted metabolites as the primary factors, while volatile organic compounds showed no activity. Greenhouse experiments with Meloidogyne incognita in tomato plants showed improved growth parameters (fresh root and shoot weight) with supernatant treatment, though nematode reduction was not statistically significant. Whole-genome sequencing of INTA L401-1 produced a 4.96 Mb draft genome, confirming its identity and revealing genes encoding nematicidal proteins (e.g. serralysin, chitinases) and bioactive compounds (e.g. serrawettins, siderophores). These findings highlight its dual roles in nematode control and plant growth promotion. The isolation of INTA L401-1 from an unusual niche raises questions about its ecological role, whether as an endosymbiont or an environmental acquisition. Field trials are needed to validate its potential as a biocontrol agent and plant growth-promoting bacterium for sustainable agriculture. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24379 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA243792025-10-30T10:45:59Z Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol Salas, Augusto Ortiz, Leila Melina Niz, José María Magariños, Francisco Del Valle, Eleodoro Eduardo Achinelly, María Fernanda Sauka, Diego Hernan Biological Control Control Biológico Serratia Meloidogyne incognita Panagrellus Heterorhabditis bacteriophora Serratia odorifera Panagrellus redivivus serrawettins serralysin Nemátodos The increasing demand for sustainable agriculture has driven the search for biological control agents targeting plant-parasitic nematodes. This study characterised Serratia odorifera strain INTA L401-1, isolated for the first time from the hemolymph of Tenebrio molitor larvae infected with Heterorhabditis bacteriophora, and evaluated its nematicidal activity. Phylogenetic analysis using 16S rRNA sequencing, along with phenotypic profiling, identified the strain as S. odorifera with 99% confidence. In vitro bioassays demonstrated significant activity against Panagrellus redivivus, with 24-hour-old cultures achieving 89.1% mortality on nutrient agar, attributed to peak secondary metabolite production. Mortality declined in 48–72-hour-old cultures but increased after seven days, likely due to metabolite accumulation. Filtered supernatants of nutrient broth cultures retained nematicidal activity (>40%), implicating secreted metabolites as the primary factors, while volatile organic compounds showed no activity. Greenhouse experiments with Meloidogyne incognita in tomato plants showed improved growth parameters (fresh root and shoot weight) with supernatant treatment, though nematode reduction was not statistically significant. Whole-genome sequencing of INTA L401-1 produced a 4.96 Mb draft genome, confirming its identity and revealing genes encoding nematicidal proteins (e.g. serralysin, chitinases) and bioactive compounds (e.g. serrawettins, siderophores). These findings highlight its dual roles in nematode control and plant growth promotion. The isolation of INTA L401-1 from an unusual niche raises questions about its ecological role, whether as an endosymbiont or an environmental acquisition. Field trials are needed to validate its potential as a biocontrol agent and plant growth-promoting bacterium for sustainable agriculture. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Salas, Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA) (G.V. al IABIMO); Argentina Fil: Salas, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ortiz, Leila Melina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Magariños, Francisco. Hospital Interzonal General de Agudos “Luisa C. de Gandulfo”; Argentina Fil: Del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. ICIAGRO Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. ICIAGRO Litoral; Argentina Fil: Achinelly, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Achinelly, María Fernanda. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina. Provincia de Buenos Aires. Comisión de Investigaciones Científicas; Argentina Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-10-30T10:30:01Z 2025-10-30T10:30:01Z 2025-06-17 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24379 https://www.tandfonline.com/doi/10.1080/09583157.2025.2520250 0958-3157 1360-0478 https://doi.org/10.1080/09583157.2025.2520250 eng info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Biocontrol Science and Technology 35 (7) : 822-837 (June 2025) |
| spellingShingle | Biological Control Control Biológico Serratia Meloidogyne incognita Panagrellus Heterorhabditis bacteriophora Serratia odorifera Panagrellus redivivus serrawettins serralysin Nemátodos Salas, Augusto Ortiz, Leila Melina Niz, José María Magariños, Francisco Del Valle, Eleodoro Eduardo Achinelly, María Fernanda Sauka, Diego Hernan Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol |
| title | Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol |
| title_full | Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol |
| title_fullStr | Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol |
| title_full_unstemmed | Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol |
| title_short | Characterisation and potential applications of Serratia odorifera INTA L401-1 in nematode biocontrol |
| title_sort | characterisation and potential applications of serratia odorifera inta l401 1 in nematode biocontrol |
| topic | Biological Control Control Biológico Serratia Meloidogyne incognita Panagrellus Heterorhabditis bacteriophora Serratia odorifera Panagrellus redivivus serrawettins serralysin Nemátodos |
| url | http://hdl.handle.net/20.500.12123/24379 https://www.tandfonline.com/doi/10.1080/09583157.2025.2520250 https://doi.org/10.1080/09583157.2025.2520250 |
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