Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae)
Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x =...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://compcytogen.pensoft.net/articles.php?id=10362 http://hdl.handle.net/20.500.12123/2437 https://doi.org/10.3897/CompCytogen.v10i4.10362 |
| _version_ | 1855483056202186752 |
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| author | Sosa, María de las Mercedes Angulo, María B. Greppi, Julian Alejandro Bugallo, Veronica Lucia |
| author_browse | Angulo, María B. Bugallo, Veronica Lucia Greppi, Julian Alejandro Sosa, María de las Mercedes |
| author_facet | Sosa, María de las Mercedes Angulo, María B. Greppi, Julian Alejandro Bugallo, Veronica Lucia |
| author_sort | Sosa, María de las Mercedes |
| collection | INTA Digital |
| description | Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for M. flagellaris (Chamisso & Schlechtendal, 1827) (2n = 22), M. grandiflora (Bentham) Pennell, 1946 (2n = 22), M. kamogawae Greppi & Hagiwara, 2011 (2n = 66), and Mecardonia sp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardonia and appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus. |
| format | Artículo |
| id | INTA2437 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA24372019-06-06T17:01:48Z Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) Sosa, María de las Mercedes Angulo, María B. Greppi, Julian Alejandro Bugallo, Veronica Lucia Plantaginaceae Genética ADN Cromosomas Genetics DNA Chromosomes Mecardonia Cytogenetic characterization and determination of DNA content by flow cytometry of five species of Mecardonia Ruiz et Pavon, 1798 (Gratiolae, Plantaginaceae) was performed. This is the first study of nuclear DNA content carried out in the genus. Mitotic analysis revealed a base chromosome number x = 11 for all entities and different ploidy levels, ranging from diploid (2n = 2x = 22) to hexaploid (2n = 6x = 66). The results include the first report of the chromosome numbers for M. flagellaris (Chamisso & Schlechtendal, 1827) (2n = 22), M. grandiflora (Bentham) Pennell, 1946 (2n = 22), M. kamogawae Greppi & Hagiwara, 2011 (2n = 66), and Mecardonia sp. (2n = 44). The three ploidy levels here reported suggest that polyploidy is common in Mecardonia and appear to be an important factor in the evolution of this genus. The 2C- and 1Cx-values were also estimated in all the species. The 2C-values ranged from 1.91 to 5.29 pg. The 1Cx-values ranged from 0.88 to 1.03 pg. The general tendency indicated a decrease in the 1Cx-value with increasing ploidy level. The significance of the results is discussed in relation to taxonomy of the genus. Instituto de Floricultura Fil: Sosa, María de las Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botanica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botanica del Nordeste; Argentina Fil: Angulo, María B. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botanica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botanica del Nordeste; Argentina Fil: Greppi, Julian Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina Fil: Bugallo, Verónica Lucía. Universidad de Buenos Aires. Facultad de Agronomía; Argentina 2018-05-21T16:44:09Z 2018-05-21T16:44:09Z 2016-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://compcytogen.pensoft.net/articles.php?id=10362 http://hdl.handle.net/20.500.12123/2437 1993-0771 1993-078X https://doi.org/10.3897/CompCytogen.v10i4.10362 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Comparative Cytogenetics 10 (4) : 769-780. (December 2016) |
| spellingShingle | Plantaginaceae Genética ADN Cromosomas Genetics DNA Chromosomes Mecardonia Sosa, María de las Mercedes Angulo, María B. Greppi, Julian Alejandro Bugallo, Veronica Lucia Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
| title | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
| title_full | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
| title_fullStr | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
| title_full_unstemmed | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
| title_short | Chromosome numbers and DNA content in some species of Mecardonia (Gratiolae, Plantaginaceae) |
| title_sort | chromosome numbers and dna content in some species of mecardonia gratiolae plantaginaceae |
| topic | Plantaginaceae Genética ADN Cromosomas Genetics DNA Chromosomes Mecardonia |
| url | https://compcytogen.pensoft.net/articles.php?id=10362 http://hdl.handle.net/20.500.12123/2437 https://doi.org/10.3897/CompCytogen.v10i4.10362 |
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