Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
Leaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with bl...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12942 http://hdl.handle.net/20.500.12123/2423 https://doi.org/10.1111/pce.12942 |
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| author | Svetaz, Laura Bustamante, Claudia Anabel Goldy, Camila Rivero, Nery Müller, Gabriela L. Valentini, Gabriel Hugo Fernie, Alisdair R. Drincovich, María Fabiana Lara, María Valeria |
| author_browse | Bustamante, Claudia Anabel Drincovich, María Fabiana Fernie, Alisdair R. Goldy, Camila Lara, María Valeria Müller, Gabriela L. Rivero, Nery Svetaz, Laura Valentini, Gabriel Hugo |
| author_facet | Svetaz, Laura Bustamante, Claudia Anabel Goldy, Camila Rivero, Nery Müller, Gabriela L. Valentini, Gabriel Hugo Fernie, Alisdair R. Drincovich, María Fabiana Lara, María Valeria |
| author_sort | Svetaz, Laura |
| collection | INTA Digital |
| description | Leaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 h post inoculation (h.p.i.). Fungal dimorphism to the filamentous form and induction of reactive oxygen species (ROS), callose synthesis, cell death and defence compound production were observed independently of the genotype. Fungal load significantly decreased after 120 h.p.i. in the resistant genotype, while the pathogen tended to grow in the susceptible genotypes. Metabolic profiling revealed a biphasic re‐programming of plant tissue in susceptible genotypes, with an initial stage co‐incident with the yeast form of the fungus and a second when the hypha is developed. Transcriptional analysis of PRs and plant hormone‐related genes indicated that pathogenesis‐related (PR) proteins are involved in P. persica defence responses against T. deformans and that salicylic acid is induced in the resistant genotype. Conducted experiments allowed the elucidation of common and differential responses in susceptible versus resistant genotypes and thus allow us to construct a picture of early events during T. deformans infection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2423 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA24232019-06-06T17:12:51Z Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes Svetaz, Laura Bustamante, Claudia Anabel Goldy, Camila Rivero, Nery Müller, Gabriela L. Valentini, Gabriel Hugo Fernie, Alisdair R. Drincovich, María Fabiana Lara, María Valeria Relaciones Huesped Patógeno Taphrina deformans Prunus Persica Rizado de las Hojas Enfermedades de las Plantas Flavonoides Ácido Salicílico Host Pathogen Relations Leaf Curls Plant Diseases Flavonoids Salicylic Acids Durazno Leaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 h post inoculation (h.p.i.). Fungal dimorphism to the filamentous form and induction of reactive oxygen species (ROS), callose synthesis, cell death and defence compound production were observed independently of the genotype. Fungal load significantly decreased after 120 h.p.i. in the resistant genotype, while the pathogen tended to grow in the susceptible genotypes. Metabolic profiling revealed a biphasic re‐programming of plant tissue in susceptible genotypes, with an initial stage co‐incident with the yeast form of the fungus and a second when the hypha is developed. Transcriptional analysis of PRs and plant hormone‐related genes indicated that pathogenesis‐related (PR) proteins are involved in P. persica defence responses against T. deformans and that salicylic acid is induced in the resistant genotype. Conducted experiments allowed the elucidation of common and differential responses in susceptible versus resistant genotypes and thus allow us to construct a picture of early events during T. deformans infection. EEA San Pedro Fil: Svetaz, Laura A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Fil: Goldy, Camila. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Fil: Rivero, Nery. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina. Fil: Fernie, Alisdair R. Planck-Institut für Molekulare Pflanzenphysiologie; Alemania Fil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. 2018-05-17T17:42:16Z 2018-05-17T17:42:16Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12942 http://hdl.handle.net/20.500.12123/2423 1365-3040 0140-7791 https://doi.org/10.1111/pce.12942 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant, cell and environment 40 (8) : 1456–1473. (2017) |
| spellingShingle | Relaciones Huesped Patógeno Taphrina deformans Prunus Persica Rizado de las Hojas Enfermedades de las Plantas Flavonoides Ácido Salicílico Host Pathogen Relations Leaf Curls Plant Diseases Flavonoids Salicylic Acids Durazno Svetaz, Laura Bustamante, Claudia Anabel Goldy, Camila Rivero, Nery Müller, Gabriela L. Valentini, Gabriel Hugo Fernie, Alisdair R. Drincovich, María Fabiana Lara, María Valeria Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes |
| title | Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes |
| title_full | Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes |
| title_fullStr | Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes |
| title_full_unstemmed | Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes |
| title_short | Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes |
| title_sort | unravelling early events in the taphrina deformans prunus persica interaction an insight into the differential responses in resistant and susceptible genotypes |
| topic | Relaciones Huesped Patógeno Taphrina deformans Prunus Persica Rizado de las Hojas Enfermedades de las Plantas Flavonoides Ácido Salicílico Host Pathogen Relations Leaf Curls Plant Diseases Flavonoids Salicylic Acids Durazno |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12942 http://hdl.handle.net/20.500.12123/2423 https://doi.org/10.1111/pce.12942 |
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