Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles
Eggs and embryo manipulation is an important biotechnological challenge to enable positioning, entrapment, and selection of reproductive cells to advance into a new era of nature-like assisted reproductive technologies. Oviductin (OVGP1) is an abundant protein in the oviduct that binds reversibly to...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/24211 https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202306901 https://doi.org/10.1002/advs.202306901 |
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| author | García Vázquez, Francisco Alberto Garrappa, Gabriela Luongo, Chiara Hamze, Julieta Gabriela Caballero, María Marco-Jiménez, Francisco Antón, José Salvador Vicente Molina-Cuberos, Gregorio J. Jiménez Movilla, María |
| author_browse | Antón, José Salvador Vicente Caballero, María García Vázquez, Francisco Alberto Garrappa, Gabriela Hamze, Julieta Gabriela Jiménez Movilla, María Luongo, Chiara Marco-Jiménez, Francisco Molina-Cuberos, Gregorio J. |
| author_facet | García Vázquez, Francisco Alberto Garrappa, Gabriela Luongo, Chiara Hamze, Julieta Gabriela Caballero, María Marco-Jiménez, Francisco Antón, José Salvador Vicente Molina-Cuberos, Gregorio J. Jiménez Movilla, María |
| author_sort | García Vázquez, Francisco Alberto |
| collection | INTA Digital |
| description | Eggs and embryo manipulation is an important biotechnological challenge to enable positioning, entrapment, and selection of reproductive cells to advance into a new era of nature-like assisted reproductive technologies. Oviductin (OVGP1) is an abundant protein in the oviduct that binds reversibly to the zona pellucida, an extracellular matrix that surrounds eggs and embryos. Here, the study reports a new method coupling OVGP1 to magnetic nanoparticles (NP) forming a complex (NPOv). NPOv specifically surrounds eggs and embryos in a reversible manner. Eggs/embryos bound to NPOv can be moved or retained when subjected to a magnetic force, and interestingly only mature-competent eggs are attracted. This procedure is compatible with normal development following gametes function, in vitro fertilization, embryo development and resulting in the birth of healthy offspring. The results provide in vitro proof-of-concept that eggs and embryos can be precisely guided in the absence of physical contact by the use of magnets. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24211 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA242112025-10-20T10:45:48Z Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles García Vázquez, Francisco Alberto Garrappa, Gabriela Luongo, Chiara Hamze, Julieta Gabriela Caballero, María Marco-Jiménez, Francisco Antón, José Salvador Vicente Molina-Cuberos, Gregorio J. Jiménez Movilla, María Nanobiotecnología Magnetismo Nanopartículas Embrión Animal Nanobiotechnology Magnetism Nanoparticles Animal Embryos Eggs and embryo manipulation is an important biotechnological challenge to enable positioning, entrapment, and selection of reproductive cells to advance into a new era of nature-like assisted reproductive technologies. Oviductin (OVGP1) is an abundant protein in the oviduct that binds reversibly to the zona pellucida, an extracellular matrix that surrounds eggs and embryos. Here, the study reports a new method coupling OVGP1 to magnetic nanoparticles (NP) forming a complex (NPOv). NPOv specifically surrounds eggs and embryos in a reversible manner. Eggs/embryos bound to NPOv can be moved or retained when subjected to a magnetic force, and interestingly only mature-competent eggs are attracted. This procedure is compatible with normal development following gametes function, in vitro fertilization, embryo development and resulting in the birth of healthy offspring. The results provide in vitro proof-of-concept that eggs and embryos can be precisely guided in the absence of physical contact by the use of magnets. EEA Rafaela Fil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); España Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; España Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Garrappa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Luongo, Chiara. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Hamze, Julieta Gabriela. Instituto Murciano de Investigación Biosanitaria (IMIB); España Fil: Hamze, Julieta Gabriela. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; España Fil: Caballero, María. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Caballero, María. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; España Fil: Marco-Jiménez, Francisco. Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal; España Fil: Antón, José Salvador Vicente. Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal; España Fil: Molina-Cuberos, Gregorio J. Universidad de Murcia. Facultad de Química. Departamento de Electromagnetismo y Electrónica; España Fil: Jimenez Movilla, Maria. Instituto Murciano de Investigación Biosanitaria (IMIB); España Fil: Jimenez Movilla, Maria. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; España 2025-10-20T10:43:57Z 2025-10-20T10:43:57Z 2024-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24211 https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202306901 2198-3844 https://doi.org/10.1002/advs.202306901 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Advanced Science 11 (18) : 2306901. (May 2024) |
| spellingShingle | Nanobiotecnología Magnetismo Nanopartículas Embrión Animal Nanobiotechnology Magnetism Nanoparticles Animal Embryos García Vázquez, Francisco Alberto Garrappa, Gabriela Luongo, Chiara Hamze, Julieta Gabriela Caballero, María Marco-Jiménez, Francisco Antón, José Salvador Vicente Molina-Cuberos, Gregorio J. Jiménez Movilla, María Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles |
| title | Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles |
| title_full | Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles |
| title_fullStr | Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles |
| title_full_unstemmed | Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles |
| title_short | Magnetic-Assisted Control of Eggs and Embryos via Zona Pellucida-Linked Nanoparticles |
| title_sort | magnetic assisted control of eggs and embryos via zona pellucida linked nanoparticles |
| topic | Nanobiotecnología Magnetismo Nanopartículas Embrión Animal Nanobiotechnology Magnetism Nanoparticles Animal Embryos |
| url | http://hdl.handle.net/20.500.12123/24211 https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202306901 https://doi.org/10.1002/advs.202306901 |
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