Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina
The present investigation conducted a 5-year longitudinal survey (August 2018–March 2023) of enteric viruses in bivalve mollusks (n = 390) collected from Golfo Nuevo, Puerto Madryn, Argentina. Samples were processed following ISO 15216-1:2017 guidelines. Norovirus genogroups I/II (NoV GI/GII), rotav...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Institute of Food Technologists (IFT)
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24151 https://ift.onlinelibrary.wiley.com/doi/10.1111/1750-3841.70573 https://doi.org/10.1111/1750-3841.70573 |
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| author | Frydman, Camila Ayelén Miño, Samuel Barbieri, Elena Susana Galeano, Fabiana Solange Vaudagna, Sergio Ramon Parreño, Gladys Viviana Mozgovoj, Marina Valeria |
| author_browse | Barbieri, Elena Susana Frydman, Camila Ayelén Galeano, Fabiana Solange Miño, Samuel Mozgovoj, Marina Valeria Parreño, Gladys Viviana Vaudagna, Sergio Ramon |
| author_facet | Frydman, Camila Ayelén Miño, Samuel Barbieri, Elena Susana Galeano, Fabiana Solange Vaudagna, Sergio Ramon Parreño, Gladys Viviana Mozgovoj, Marina Valeria |
| author_sort | Frydman, Camila Ayelén |
| collection | INTA Digital |
| description | The present investigation conducted a 5-year longitudinal survey (August 2018–March 2023) of enteric viruses in bivalve mollusks (n = 390) collected from Golfo Nuevo, Puerto Madryn, Argentina. Samples were processed following ISO 15216-1:2017 guidelines. Norovirus genogroups I/II (NoV GI/GII), rotavirus A (RVA), hepatitis A (HAV) and E (HEV) viruses, and adenovirus (AdV) were detected and quantified by RT-qPCR/qPCR, genotyped, and subjected to phylogenetic analysis. At least one virus was detected in 40.7% of composite samples. AdV exhibited the highest prevalence (56.6%), while NoV GII and RVA were detected in 12.4% of samples; HEV and HAV were detected in 5.3% and 4.4%, respectively, and NoV GI was not detected. Mean viral loads ranged from 4.8 to 6.3 log10 genomic copies/g of bivalve mollusks. Temporal trends revealed a significant decline in detection post-pandemic for RVA, NoV GII, and HAV, while AdV and HEV rates remained stable. Genotyping identified pandemic variant NoV GII.4[P16], RVA G8P[1], HAV IA, and AdV A31. These results provide the first comprehensive baseline of enteric virus contamination in Patagonian shellfish. Routine virus monitoring and evidence-based control measures are therefore imperative to safeguard public health and to align Argentine bivalve mollusks with international safety standards. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24151 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Institute of Food Technologists (IFT) |
| publisherStr | Institute of Food Technologists (IFT) |
| record_format | dspace |
| spelling | INTA241512025-10-13T14:02:12Z Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina Frydman, Camila Ayelén Miño, Samuel Barbieri, Elena Susana Galeano, Fabiana Solange Vaudagna, Sergio Ramon Parreño, Gladys Viviana Mozgovoj, Marina Valeria Viruses Shellfish Mollusca Bivalvia Food Contamination Virus Argentina Marisco Bivalvo Contaminación Alimentaria Molecular Surveillance Vigilancia Molecular The present investigation conducted a 5-year longitudinal survey (August 2018–March 2023) of enteric viruses in bivalve mollusks (n = 390) collected from Golfo Nuevo, Puerto Madryn, Argentina. Samples were processed following ISO 15216-1:2017 guidelines. Norovirus genogroups I/II (NoV GI/GII), rotavirus A (RVA), hepatitis A (HAV) and E (HEV) viruses, and adenovirus (AdV) were detected and quantified by RT-qPCR/qPCR, genotyped, and subjected to phylogenetic analysis. At least one virus was detected in 40.7% of composite samples. AdV exhibited the highest prevalence (56.6%), while NoV GII and RVA were detected in 12.4% of samples; HEV and HAV were detected in 5.3% and 4.4%, respectively, and NoV GI was not detected. Mean viral loads ranged from 4.8 to 6.3 log10 genomic copies/g of bivalve mollusks. Temporal trends revealed a significant decline in detection post-pandemic for RVA, NoV GII, and HAV, while AdV and HEV rates remained stable. Genotyping identified pandemic variant NoV GII.4[P16], RVA G8P[1], HAV IA, and AdV A31. These results provide the first comprehensive baseline of enteric virus contamination in Patagonian shellfish. Routine virus monitoring and evidence-based control measures are therefore imperative to safeguard public health and to align Argentine bivalve mollusks with international safety standards. La presente investigación realizó un estudio longitudinal de 5 años (agosto de 2018 a marzo de 2023) de virus entéricos en moluscos bivalvos (n = 390) recolectados en el Golfo Nuevo, Puerto Madryn, Argentina. Las muestras se procesaron siguiendo las directrices de la norma ISO 15216-1:2017. Se detectaron y cuantificaron mediante RT-qPCR/qPCR los genogrupos I/II de norovirus (NoV GI/GII), rotavirus A (RVA), virus de la hepatitis A (VHA) y E (VHE) y adenovirus (AdV), se genotipificaron y se sometieron a análisis filogenético. Se detectó al menos un virus en el 40,7% de las muestras compuestas. El AdV presentó la prevalencia más alta (56,6%), mientras que NoV GII y RVA se detectaron en el 12,4% de las muestras; el VHE y el VHA se detectaron en el 5,3% y el 4,4%, respectivamente, y no se detectó NoV GI. Las cargas virales medias oscilaron entre 4,8 y 6,3 log10 copias genómicas/g en moluscos bivalvos. Las tendencias temporales revelaron una disminución significativa en la detección pospandémica de RVA, NoV GII y VHA, mientras que las tasas de AdV y VHE se mantuvieron estables. La genotipificación identificó la variante pandémica NoV GII.4[P16], RVA G8P[1], VHA IA y AdV A31. Estos resultados proporcionan la primera línea de base completa de contaminación por virus entéricos en mariscos patagónicos. Por lo tanto, el monitoreo rutinario del virus y las medidas de control basadas en la evidencia son imperativos para salvaguardar la salud pública y alinear los moluscos bivalvos argentinos con las normas internacionales de seguridad. Instituto de Investigación Tecnología de Alimentos (ITA) Fil: Frydman, Camila Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Fil: Frydman, Camila Ayelén. Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS) UEDD INTA-CONICET; Argentina. Fil: Frydman, Camila Ayelén. Universidad Nacional de Hurlingham; Argentina. Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina. Fil: Barbieri, Elena Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro para el Estudio de Sistemas Marinos; Argentina. Fil: Galeano, Fabiana Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Fil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Fil: Vaudagna, Sergio Ramon. Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS) UEDD INTA-CONICET; Argentina. Fil: Vaudagna, Sergio Ramon. Universidad Nacional de Hurlingham; Argentina. Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnologícas (IVIT). INCUINTA UEDD INTA-CONICET; Argentina. Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina. Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Fil: Mozgovoj, Marina Valeria. Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS) UEDD INTA-CONICET; Argentina. Fil: Mozgovoj, Marina Valeria. Universidad Nacional de Hurlingham; Argentina. 2025-10-13T13:49:04Z 2025-10-13T13:49:04Z 2025-09-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24151 https://ift.onlinelibrary.wiley.com/doi/10.1111/1750-3841.70573 0022-1147 (Print) 1750-3841 (Online) https://doi.org/10.1111/1750-3841.70573 eng info:eu-repograntAgreement/INTA/2023-PE-L04-I120, Inocuidad física, química y biológica de los alimentos. Metodologías analíticas, tecnologías, estrategias de intervención innovadoras y análisis de riesgo info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Nuevo, Golfo .......... (bay) (World, South America, Argentina, Chubut) 1113522 01/08/2018 - 01/03/2023 Institute of Food Technologists (IFT) Journal of Food Science 90 (10) : e70573. (October 2025) |
| spellingShingle | Viruses Shellfish Mollusca Bivalvia Food Contamination Virus Argentina Marisco Bivalvo Contaminación Alimentaria Molecular Surveillance Vigilancia Molecular Frydman, Camila Ayelén Miño, Samuel Barbieri, Elena Susana Galeano, Fabiana Solange Vaudagna, Sergio Ramon Parreño, Gladys Viviana Mozgovoj, Marina Valeria Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina |
| title | Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina |
| title_full | Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina |
| title_fullStr | Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina |
| title_full_unstemmed | Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina |
| title_short | Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina |
| title_sort | five year molecular surveillance reveals widespread foodborne enteric viruses in bivalve mollusks from golfo nuevo argentina |
| topic | Viruses Shellfish Mollusca Bivalvia Food Contamination Virus Argentina Marisco Bivalvo Contaminación Alimentaria Molecular Surveillance Vigilancia Molecular |
| url | http://hdl.handle.net/20.500.12123/24151 https://ift.onlinelibrary.wiley.com/doi/10.1111/1750-3841.70573 https://doi.org/10.1111/1750-3841.70573 |
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