First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and molecular analyses were performed. The results of the biochemical blood analy...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24056 https://www.mdpi.com/3042-4526/2/3/35 https://doi.org/10.3390/wild2030035 |
| _version_ | 1855487251297861632 |
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| author | Sebastian, Patrick Vaschalde, Paula Josefina Pelosi, María Celeste Flores, Fernando Sebastián Yedro, Julio Tarragona, Evelina Luisa |
| author_browse | Flores, Fernando Sebastián Pelosi, María Celeste Sebastian, Patrick Tarragona, Evelina Luisa Vaschalde, Paula Josefina Yedro, Julio |
| author_facet | Sebastian, Patrick Vaschalde, Paula Josefina Pelosi, María Celeste Flores, Fernando Sebastián Yedro, Julio Tarragona, Evelina Luisa |
| author_sort | Sebastian, Patrick |
| collection | INTA Digital |
| description | In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and molecular analyses were performed. The results of the biochemical blood analysis and the clinical signs were compatible with theileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. The phylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted in the identification of the detected strain as a Theileria genospecies closely related to Theileria spp. detected in other deer species. Further, a genospecies of Anaplasma was detected in the blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum. The results of this study demonstrate co-infection with two novel genospecies of Anaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time in Argentina. However, the pathogenicity of these strains and specific role of S. gouazoubira in their enzootic transmission cycles remains unclear. To improve epidemiological understanding, assess risks and develop targeted control strategies, future studies should prioritize the isolation, characterization and cultivation of these genospecies and the assessment of vector competence. |
| format | Artículo |
| id | INTA24056 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA240562025-10-03T12:07:42Z First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina Sebastian, Patrick Vaschalde, Paula Josefina Pelosi, María Celeste Flores, Fernando Sebastián Yedro, Julio Tarragona, Evelina Luisa Theileria Anaplasma Cervidae Ticks Identification Entre Rios Garrapata Identificación Subulo gouazoubira In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilation and recumbency without the ability to maintain sternal or ambulatory posture. Based on the symptoms, serological, biochemical and molecular analyses were performed. The results of the biochemical blood analysis and the clinical signs were compatible with theileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. The phylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted in the identification of the detected strain as a Theileria genospecies closely related to Theileria spp. detected in other deer species. Further, a genospecies of Anaplasma was detected in the blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum. The results of this study demonstrate co-infection with two novel genospecies of Anaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time in Argentina. However, the pathogenicity of these strains and specific role of S. gouazoubira in their enzootic transmission cycles remains unclear. To improve epidemiological understanding, assess risks and develop targeted control strategies, future studies should prioritize the isolation, characterization and cultivation of these genospecies and the assessment of vector competence. EEA Rafaela Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina Fil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina Fil: Pelosi, María Celeste. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Zoología, Diversidad y Ambiente; Argentina Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina Fil: Yedro, Julio. Gobierno de la Provincia de Entre Ríos. Dirección de Recursos Naturales; Argentina Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2025-10-03T12:03:18Z 2025-10-03T12:03:18Z 2025-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24056 https://www.mdpi.com/3042-4526/2/3/35 3042-4526 https://doi.org/10.3390/wild2030035 eng info:eu-repograntAgreement/INTA/2023-PE-L01-I044, Enfermedades subtropicales con impacto sobre la producción, salud pública y sustentabilidad en la ganadería del norte de Argentina info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Wild 2 (3) : 35. (September 2025) |
| spellingShingle | Theileria Anaplasma Cervidae Ticks Identification Entre Rios Garrapata Identificación Subulo gouazoubira Sebastian, Patrick Vaschalde, Paula Josefina Pelosi, María Celeste Flores, Fernando Sebastián Yedro, Julio Tarragona, Evelina Luisa First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina |
| title | First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina |
| title_full | First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina |
| title_fullStr | First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina |
| title_full_unstemmed | First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina |
| title_short | First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina |
| title_sort | first molecular evidence of theileria and anaplasma genospecies in subulo gouazoubira exhibiting clinical symptoms from entre rios province argentina |
| topic | Theileria Anaplasma Cervidae Ticks Identification Entre Rios Garrapata Identificación Subulo gouazoubira |
| url | http://hdl.handle.net/20.500.12123/24056 https://www.mdpi.com/3042-4526/2/3/35 https://doi.org/10.3390/wild2030035 |
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