Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology
Cattle abortions pose a serious challenge to livestock health and farm income, yet many cases lack a clear cause even when signs of infection are present. We examined immune activity in aborted bovine fetuses at mid and late gestation caused by three common agents that invade cells, comparing them w...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Multidisciplinary Digital Publishing Institute, MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/24019 https://www.mdpi.com/2076-2615/15/19/2878 https://doi.org/10.3390/ani15192878 |
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| author | Sosa, Emiliano Pla, Natalia Moore, Prando Dadin Garcia, Juan Agustin Campero, Lucia Maria Fiorentino, María Andrea Miqueo, Evangelina González Altamiranda, Erika Lázaro, Fermín Morán, Karen Bilbao, María Guillermina Quintana, Silvina Marin, Maia Solange Canton, German Jose |
| author_browse | Bilbao, María Guillermina Campero, Lucia Maria Canton, German Jose Fiorentino, María Andrea Garcia, Juan Agustin González Altamiranda, Erika Lázaro, Fermín Marin, Maia Solange Miqueo, Evangelina Moore, Prando Dadin Morán, Karen Pla, Natalia Quintana, Silvina Sosa, Emiliano |
| author_facet | Sosa, Emiliano Pla, Natalia Moore, Prando Dadin Garcia, Juan Agustin Campero, Lucia Maria Fiorentino, María Andrea Miqueo, Evangelina González Altamiranda, Erika Lázaro, Fermín Morán, Karen Bilbao, María Guillermina Quintana, Silvina Marin, Maia Solange Canton, German Jose |
| author_sort | Sosa, Emiliano |
| collection | INTA Digital |
| description | Cattle abortions pose a serious challenge to livestock health and farm income, yet many cases lack a clear cause even when signs of infection are present. We examined immune activity in aborted bovine fetuses at mid and late gestation caused by three common agents that invade cells, comparing them with fetuses showing infection-like lesions without a known cause and with healthy controls. In mid-gestation losses by intracellular agents, we found a surge in proteins that drive inflammation, indicating a strong early defensive reaction. In contrast, late-pregnancy losses showed a drop in those inflammatory signals, suggesting a shift toward a more subdued immune state. Fetuses with unknown infections also showed distinct patterns. Although differences in haptoglobin levels were observed in mid-gestation fetuses aborted due to intracellular agents compared to controls, the overall concentration remained low and may be of limited biological relevance. Variations in the fetal immune response according to pathogen type and gestational stage provide novel insights into the pathogenesis of key intracellular agents, thereby strengthening diagnostic approaches and contributing to improved herd health and agricultural sustainability.
Intracellular pathogens such as Neospora caninum, Brucella abortus, and Bovine Viral Diarrhea Virus (BVDV) are major contributors to bovine abortions, yet many cases remain without a definitive etiological diagnosis despite inflammatory evidence. This study aimed to
characterize the immune response in bovine fetuses aborted due to these intracellular
agents, comparing them with fetuses showing inflammatory lesions of probable infectious origin and with negative controls. We analyzed cytokine expression (IFN-γ, TNFα, IL- 4, IL-8, IL-12) and haptoglobin levels in mid- and late-gestation fetuses. Mid-gestation fetuses infected with intracellular agents exhibited elevated IFN-γ and IL-8 expressions,suggesting a Th1-type immune response, while late-gestation fetuses showed decreased of these cytokines, indicating a shift toward a Th2-type response. Probable infectious abortions at late gestation also showed downregulation of IFN-γ. No significant differences were observed in TNF-α and IL-12 expressions. Additionally, haptoglobin levels were lower in mid-gestation infected fetuses compared to controls. These findings highlight gestational age-dependent immune modulation in response to intracellular infections and suggest that other unidentified pathogens may contribute to abortions with inflammatory lesions but no confirmed etiology. This study enhances our understanding of fetal immune responses in bovine abortions and may support improved diagnostic approaches for reproductive losses in cattle. |
| format | info:ar-repo/semantics/artículo |
| id | INTA24019 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Multidisciplinary Digital Publishing Institute, MDPI |
| publisherStr | Multidisciplinary Digital Publishing Institute, MDPI |
| record_format | dspace |
| spelling | INTA240192025-10-01T14:34:13Z Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology Sosa, Emiliano Pla, Natalia Moore, Prando Dadin Garcia, Juan Agustin Campero, Lucia Maria Fiorentino, María Andrea Miqueo, Evangelina González Altamiranda, Erika Lázaro, Fermín Morán, Karen Bilbao, María Guillermina Quintana, Silvina Marin, Maia Solange Canton, German Jose Ganado Bovino Aborto Neospora caninum Brucella abortus Pestivirus de la Diarrea Bovina Respuesta Inmunológica Cattle Abortion Bovine Diarrhoea Pestivirus Immune Response Cytokines Citoquinas Cattle abortions pose a serious challenge to livestock health and farm income, yet many cases lack a clear cause even when signs of infection are present. We examined immune activity in aborted bovine fetuses at mid and late gestation caused by three common agents that invade cells, comparing them with fetuses showing infection-like lesions without a known cause and with healthy controls. In mid-gestation losses by intracellular agents, we found a surge in proteins that drive inflammation, indicating a strong early defensive reaction. In contrast, late-pregnancy losses showed a drop in those inflammatory signals, suggesting a shift toward a more subdued immune state. Fetuses with unknown infections also showed distinct patterns. Although differences in haptoglobin levels were observed in mid-gestation fetuses aborted due to intracellular agents compared to controls, the overall concentration remained low and may be of limited biological relevance. Variations in the fetal immune response according to pathogen type and gestational stage provide novel insights into the pathogenesis of key intracellular agents, thereby strengthening diagnostic approaches and contributing to improved herd health and agricultural sustainability. Intracellular pathogens such as Neospora caninum, Brucella abortus, and Bovine Viral Diarrhea Virus (BVDV) are major contributors to bovine abortions, yet many cases remain without a definitive etiological diagnosis despite inflammatory evidence. This study aimed to characterize the immune response in bovine fetuses aborted due to these intracellular agents, comparing them with fetuses showing inflammatory lesions of probable infectious origin and with negative controls. We analyzed cytokine expression (IFN-γ, TNFα, IL- 4, IL-8, IL-12) and haptoglobin levels in mid- and late-gestation fetuses. Mid-gestation fetuses infected with intracellular agents exhibited elevated IFN-γ and IL-8 expressions,suggesting a Th1-type immune response, while late-gestation fetuses showed decreased of these cytokines, indicating a shift toward a Th2-type response. Probable infectious abortions at late gestation also showed downregulation of IFN-γ. No significant differences were observed in TNF-α and IL-12 expressions. Additionally, haptoglobin levels were lower in mid-gestation infected fetuses compared to controls. These findings highlight gestational age-dependent immune modulation in response to intracellular infections and suggest that other unidentified pathogens may contribute to abortions with inflammatory lesions but no confirmed etiology. This study enhances our understanding of fetal immune responses in bovine abortions and may support improved diagnostic approaches for reproductive losses in cattle. EEA Balcarce Fil: Sosa, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Pla, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Moore, Dadin Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Fiorentino, María Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Miqueo, Evangelina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: González Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Lázaro, Fermín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Morán, Karen. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Bilbao, María Guillermina. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina Fil: Marín, Maia Solange. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Marín, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina 2025-10-01T14:24:15Z 2025-10-01T14:24:15Z 2025-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/24019 https://www.mdpi.com/2076-2615/15/19/2878 2076-2615 (online) https://doi.org/10.3390/ani15192878 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinario info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Multidisciplinary Digital Publishing Institute, MDPI Animals 15 (19) : 2878 (October 2025) |
| spellingShingle | Ganado Bovino Aborto Neospora caninum Brucella abortus Pestivirus de la Diarrea Bovina Respuesta Inmunológica Cattle Abortion Bovine Diarrhoea Pestivirus Immune Response Cytokines Citoquinas Sosa, Emiliano Pla, Natalia Moore, Prando Dadin Garcia, Juan Agustin Campero, Lucia Maria Fiorentino, María Andrea Miqueo, Evangelina González Altamiranda, Erika Lázaro, Fermín Morán, Karen Bilbao, María Guillermina Quintana, Silvina Marin, Maia Solange Canton, German Jose Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology |
| title | Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology |
| title_full | Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology |
| title_fullStr | Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology |
| title_full_unstemmed | Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology |
| title_short | Cytokine Expression and Haptoglobin Levels in Bovine Fetuses Spontaneously Aborted by Intracellular Infectious Agents and by Probable Infectious Etiology |
| title_sort | cytokine expression and haptoglobin levels in bovine fetuses spontaneously aborted by intracellular infectious agents and by probable infectious etiology |
| topic | Ganado Bovino Aborto Neospora caninum Brucella abortus Pestivirus de la Diarrea Bovina Respuesta Inmunológica Cattle Abortion Bovine Diarrhoea Pestivirus Immune Response Cytokines Citoquinas |
| url | http://hdl.handle.net/20.500.12123/24019 https://www.mdpi.com/2076-2615/15/19/2878 https://doi.org/10.3390/ani15192878 |
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