Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
Boar ejaculate is composed of sperm cells and seminal plasma (SP) and is emitted in different fractions (pre-sperm fraction; spermatic-rich fraction; intermediate fraction; post-spermatic fraction), with different composition of SP and volume, which could influence the sperm quality during seminal d...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer Nature
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/23941 https://www.nature.com/articles/s41598-023-42254-3 https://doi.org/10.1038/s41598-023-42254-3 |
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| author | Luongo, Chiara Llamas-López, Pedro José Garrappa, Gabriela Rodríguez-Tobón, Ernesto Grudzinska, Paulina García Vázquez, Francisco Alberto |
| author_browse | García Vázquez, Francisco Alberto Garrappa, Gabriela Grudzinska, Paulina Llamas-López, Pedro José Luongo, Chiara Rodríguez-Tobón, Ernesto |
| author_facet | Luongo, Chiara Llamas-López, Pedro José Garrappa, Gabriela Rodríguez-Tobón, Ernesto Grudzinska, Paulina García Vázquez, Francisco Alberto |
| author_sort | Luongo, Chiara |
| collection | INTA Digital |
| description | Boar ejaculate is composed of sperm cells and seminal plasma (SP) and is emitted in different fractions (pre-sperm fraction; spermatic-rich fraction; intermediate fraction; post-spermatic fraction), with different composition of SP and volume, which could influence the sperm quality during seminal doses preparation, conservation, and interaction with the female reproductive tract. In artificial insemination (AI) centers, seminal doses are usually prepared with the spermatic-rich and intermediate fractions, but the inclusion of other ejaculate fractions, although controversial, is beginning to be applied. The objective was to evaluate the synergic effect of accumulative ejaculated fractions on sperm functionality during seminal doses preparation, throughout storage and after incubation with uterine fluid (UF). For this purpose, a total of 57 ejaculates were collected, and the following experimental groups were prepared (n = 19 per group): (F1) spermatic-rich fraction; (F2) F1 plus intermediate fraction; (F3) F2 plus post-spermatic fraction. Each group was stored for 5 days at ∼16 °C, and the following parameters were evaluated: sperm metabolism of pure and diluted semen (day 1), sperm quality parameters (days 1, 3, 5), thermal-resistance test (TRT) and incubation with uterine fluid (UF) (day 5). Sperm metabolic rates between accumulative ejaculate fractions from pure and diluted semen did not show differences. Also, sperm quality parameters were not affected by the ejaculate fraction during storage. However, sperm subjected to TRT showed similar results except for progressive motility, which was better in F2 and F3 than F1. When sperm were incubated with UF, the quality decreased in each group, but sperm from F2 and F3 were less affected than those from F1. In conclusion, the post-spermatic fraction can be included in seminal doses for their use in AI-centers, with functionality of sperm of different SP origins not being impaired throughout the storage, and responding better to thermal and UF stress. However, further research in AI-centers is necessary to test the sperm behaviour under presented conditions. |
| format | Artículo |
| id | INTA23941 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA239412025-09-25T12:24:28Z Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid Luongo, Chiara Llamas-López, Pedro José Garrappa, Gabriela Rodríguez-Tobón, Ernesto Grudzinska, Paulina García Vázquez, Francisco Alberto Verraco Cerdo Espermatozoo Reproducción Animal Calidad Boars Swine Spermatozoa Semen Animal Reproduction Quality Esperma Plasma Seminal Sperm Seminal Plasma Boar ejaculate is composed of sperm cells and seminal plasma (SP) and is emitted in different fractions (pre-sperm fraction; spermatic-rich fraction; intermediate fraction; post-spermatic fraction), with different composition of SP and volume, which could influence the sperm quality during seminal doses preparation, conservation, and interaction with the female reproductive tract. In artificial insemination (AI) centers, seminal doses are usually prepared with the spermatic-rich and intermediate fractions, but the inclusion of other ejaculate fractions, although controversial, is beginning to be applied. The objective was to evaluate the synergic effect of accumulative ejaculated fractions on sperm functionality during seminal doses preparation, throughout storage and after incubation with uterine fluid (UF). For this purpose, a total of 57 ejaculates were collected, and the following experimental groups were prepared (n = 19 per group): (F1) spermatic-rich fraction; (F2) F1 plus intermediate fraction; (F3) F2 plus post-spermatic fraction. Each group was stored for 5 days at ∼16 °C, and the following parameters were evaluated: sperm metabolism of pure and diluted semen (day 1), sperm quality parameters (days 1, 3, 5), thermal-resistance test (TRT) and incubation with uterine fluid (UF) (day 5). Sperm metabolic rates between accumulative ejaculate fractions from pure and diluted semen did not show differences. Also, sperm quality parameters were not affected by the ejaculate fraction during storage. However, sperm subjected to TRT showed similar results except for progressive motility, which was better in F2 and F3 than F1. When sperm were incubated with UF, the quality decreased in each group, but sperm from F2 and F3 were less affected than those from F1. In conclusion, the post-spermatic fraction can be included in seminal doses for their use in AI-centers, with functionality of sperm of different SP origins not being impaired throughout the storage, and responding better to thermal and UF stress. However, further research in AI-centers is necessary to test the sperm behaviour under presented conditions. Instituto de Investigación Animal del Chaco Semiárido Fil: Luongo, Chiara. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Llamas-López, Pedro José. Universidad Miguel Hernández. Departamento de Tecnología Agroalimentaria; España Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina Fil: Rodríguez-Tobón, Ernesto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Rodríguez-Tobón, Ernesto. Universidad Autónoma Metropolitana. Unidad Iztapalapa. Departamento de Biología de La Reproducción; México Fil: Grudzinska, Paulina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); España 2025-09-25T12:21:40Z 2025-09-25T12:21:40Z 2023-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23941 https://www.nature.com/articles/s41598-023-42254-3 2045-2322 https://doi.org/10.1038/s41598-023-42254-3 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Scientific Reports 13 : Article number: 15258 (2023) |
| spellingShingle | Verraco Cerdo Espermatozoo Reproducción Animal Calidad Boars Swine Spermatozoa Semen Animal Reproduction Quality Esperma Plasma Seminal Sperm Seminal Plasma Luongo, Chiara Llamas-López, Pedro José Garrappa, Gabriela Rodríguez-Tobón, Ernesto Grudzinska, Paulina García Vázquez, Francisco Alberto Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid |
| title | Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid |
| title_full | Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid |
| title_fullStr | Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid |
| title_full_unstemmed | Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid |
| title_short | Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid |
| title_sort | impact of inclusion of post spermatic ejaculate fraction in boar seminal doses on sperm metabolism quality and interaction with uterine fluid |
| topic | Verraco Cerdo Espermatozoo Reproducción Animal Calidad Boars Swine Spermatozoa Semen Animal Reproduction Quality Esperma Plasma Seminal Sperm Seminal Plasma |
| url | http://hdl.handle.net/20.500.12123/23941 https://www.nature.com/articles/s41598-023-42254-3 https://doi.org/10.1038/s41598-023-42254-3 |
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