High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio

High-pressure treatments (400 and 600 MPa) combined with the addition of sodium lactate (1 and 3%) were tested to reduce Escherichia coli O157:H7 (STEC O157) and spoilage microbiota contamination in a manufactured cured beef carpaccio in fresh or frozen conditions. Counts of spoilage microorganisms...

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Autores principales: Masana, Marcelo Oscar, Barrio, Yanina Ximena, Palladino, Pablo Martin, Sancho, Ana Maria, Vaudagna, Sergio Ramon
Formato: Artículo
Lenguaje:Inglés
Publicado: 2018
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0740002014002573
http://hdl.handle.net/20.500.12123/2383
https://doi.org/10.1016/j.fm.2014.10.007
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author Masana, Marcelo Oscar
Barrio, Yanina Ximena
Palladino, Pablo Martin
Sancho, Ana Maria
Vaudagna, Sergio Ramon
author_browse Barrio, Yanina Ximena
Masana, Marcelo Oscar
Palladino, Pablo Martin
Sancho, Ana Maria
Vaudagna, Sergio Ramon
author_facet Masana, Marcelo Oscar
Barrio, Yanina Ximena
Palladino, Pablo Martin
Sancho, Ana Maria
Vaudagna, Sergio Ramon
author_sort Masana, Marcelo Oscar
collection INTA Digital
description High-pressure treatments (400 and 600 MPa) combined with the addition of sodium lactate (1 and 3%) were tested to reduce Escherichia coli O157:H7 (STEC O157) and spoilage microbiota contamination in a manufactured cured beef carpaccio in fresh or frozen conditions. Counts of spoilage microorganisms and STEC O157 were also examined during the curing step to prepare the carpaccio. STEC O157 counts remained almost unchanged through the curing process performed at 1 ± 1 °C for 12 days, with a small decrease in samples with 3% of sodium lactate. High-pressure treatments at 600 MPa for 5 min achieved an immediate reduction of up to 2 logarithmic units of STEC O157 in frozen carpaccio, and up to 1.19 log in fresh condition. Counts of spoilage bacteria diminished below detection limits in fresh or frozen carpaccio added with sodium lactate by the application of 400 and 600 MPa. Maximum injury on STEC O157 cells was observed at 600 MPa in carpaccio in fresh condition without added sodium lactate. Lethality of high-pressure treatments on STEC O157 was enhanced in frozen carpaccio, while the addition of sodium lactate at 3% reduced the lethality on STEC O157 in frozen samples, and the degree of injury in fresh carpaccio.
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spelling INTA23832018-07-04T13:01:08Z High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio Masana, Marcelo Oscar Barrio, Yanina Ximena Palladino, Pablo Martin Sancho, Ana Maria Vaudagna, Sergio Ramon Tecnología Alta Presión Sodio Lactatos Escherichia coli Carne Salada High Pressure Technology Sodium Lactates Cured Meat Carpaccio High-pressure treatments (400 and 600 MPa) combined with the addition of sodium lactate (1 and 3%) were tested to reduce Escherichia coli O157:H7 (STEC O157) and spoilage microbiota contamination in a manufactured cured beef carpaccio in fresh or frozen conditions. Counts of spoilage microorganisms and STEC O157 were also examined during the curing step to prepare the carpaccio. STEC O157 counts remained almost unchanged through the curing process performed at 1 ± 1 °C for 12 days, with a small decrease in samples with 3% of sodium lactate. High-pressure treatments at 600 MPa for 5 min achieved an immediate reduction of up to 2 logarithmic units of STEC O157 in frozen carpaccio, and up to 1.19 log in fresh condition. Counts of spoilage bacteria diminished below detection limits in fresh or frozen carpaccio added with sodium lactate by the application of 400 and 600 MPa. Maximum injury on STEC O157 cells was observed at 600 MPa in carpaccio in fresh condition without added sodium lactate. Lethality of high-pressure treatments on STEC O157 was enhanced in frozen carpaccio, while the addition of sodium lactate at 3% reduced the lethality on STEC O157 in frozen samples, and the degree of injury in fresh carpaccio. Instituto de Tecnología de Alimentos Fil: Masana, Marcelo Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina Fil: Barrio, Yanina Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad Argentina de la Empresa. Facultad de Ingeniería y Ciencias Exactas. Departamento de Biotecnología y Tecnología Alimentaria; Argentina Fil: Palladino, Pablo Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina Fil: Sancho, Ana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina Fil: Vaudagna, Sergio Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad Argentina de la Empresa. Facultad de Ingeniería y Ciencias Exactas. Departamento de Biotecnología y Tecnología Alimentaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnica, Argentina 2018-05-14T13:02:31Z 2018-05-14T13:02:31Z 2015-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0740002014002573 http://hdl.handle.net/20.500.12123/2383 0740-0020 https://doi.org/10.1016/j.fm.2014.10.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Food microbiology 46 : 610-617. (April 2015)
spellingShingle Tecnología Alta Presión
Sodio
Lactatos
Escherichia coli
Carne Salada
High Pressure Technology
Sodium
Lactates
Cured Meat
Carpaccio
Masana, Marcelo Oscar
Barrio, Yanina Ximena
Palladino, Pablo Martin
Sancho, Ana Maria
Vaudagna, Sergio Ramon
High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio
title High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio
title_full High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio
title_fullStr High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio
title_full_unstemmed High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio
title_short High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio
title_sort high pressure treatments combined with sodium lactate to inactivate escherichia coli o157 h7 and spoilage microbiota in cured beef carpaccio
topic Tecnología Alta Presión
Sodio
Lactatos
Escherichia coli
Carne Salada
High Pressure Technology
Sodium
Lactates
Cured Meat
Carpaccio
url https://www.sciencedirect.com/science/article/pii/S0740002014002573
http://hdl.handle.net/20.500.12123/2383
https://doi.org/10.1016/j.fm.2014.10.007
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