Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review

Garlic reproduces asexually, as its flowers are generally partially or completely sterile. In some garlic populations, inflorescences often exhibit small aerial bulbs, called ‘bulbils’, which are believed to compete for photoassimilates with developing flowers, thereby influencing fertility. Recent...

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Main Authors: Dhall, Rajinder Kumar, Cavagnaro, Pablo, Malik, Geetika, Malik, Ajaz Ahmed, Rana, Neha
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Wiley 2025
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/23796
https://onlinelibrary.wiley.com/doi/10.1111/pbr.70012
https://doi.org/10.1111/pbr.70012
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author Dhall, Rajinder Kumar
Cavagnaro, Pablo
Malik, Geetika
Malik, Ajaz Ahmed
Rana, Neha
author_browse Cavagnaro, Pablo
Dhall, Rajinder Kumar
Malik, Ajaz Ahmed
Malik, Geetika
Rana, Neha
author_facet Dhall, Rajinder Kumar
Cavagnaro, Pablo
Malik, Geetika
Malik, Ajaz Ahmed
Rana, Neha
author_sort Dhall, Rajinder Kumar
collection INTA Digital
description Garlic reproduces asexually, as its flowers are generally partially or completely sterile. In some garlic populations, inflorescences often exhibit small aerial bulbs, called ‘bulbils’, which are believed to compete for photoassimilates with developing flowers, thereby influencing fertility. Recent physiological studies on a few fertile genotypes and successful restoration of fertility by removing bulbils from inflorescences and growing the plants under long day conditions have suggested the potential possibility of commercial true seed production in garlic. Additionally, advances in garlic research using cutting edge technologies, including genomic assisted breeding, QTL and simply-inherited trait mapping, genome wide association studies (GWAS), transcriptomics, metabolic engineering and somaclonal variation, have contributed to our understanding of underlying causes and mechanisms of garlic sterility, although, to date, this has not been completely elucidated. As a result, large-scale commercial seed production of garlic is not currently possible. Thus, current breeding strategies should focus on the selection of those—rather infrequent—clones with fertile flowers, and inflorescences with few and small bulbils, exhibiting high yields of large and viable seeds. This review summarizes and critically discusses results from over 30 years of research on garlic reproductive biology, its sterility and reported cases of fertility restoration, and the approaches used for true seed production in the species. Genetic, physiological and morphological factors affecting this trait are discussed. Unlocking garlic's sexual reproduction would have a tremendous positive impact in breeding, genetic research, and—ultimately—domestication of this flavourful vegetable.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2025
publishDateRange 2025
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spelling INTA237962025-09-12T13:53:27Z Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review Dhall, Rajinder Kumar Cavagnaro, Pablo Malik, Geetika Malik, Ajaz Ahmed Rana, Neha Ajo Fitomejoramiento Expresión Génica Floración Transcriptoma Garlic Plant Breeding Gene Expression Flowering Transcriptome Allium sativum Plant Biotechnology Biotecnología Vegetal Garlic reproduces asexually, as its flowers are generally partially or completely sterile. In some garlic populations, inflorescences often exhibit small aerial bulbs, called ‘bulbils’, which are believed to compete for photoassimilates with developing flowers, thereby influencing fertility. Recent physiological studies on a few fertile genotypes and successful restoration of fertility by removing bulbils from inflorescences and growing the plants under long day conditions have suggested the potential possibility of commercial true seed production in garlic. Additionally, advances in garlic research using cutting edge technologies, including genomic assisted breeding, QTL and simply-inherited trait mapping, genome wide association studies (GWAS), transcriptomics, metabolic engineering and somaclonal variation, have contributed to our understanding of underlying causes and mechanisms of garlic sterility, although, to date, this has not been completely elucidated. As a result, large-scale commercial seed production of garlic is not currently possible. Thus, current breeding strategies should focus on the selection of those—rather infrequent—clones with fertile flowers, and inflorescences with few and small bulbils, exhibiting high yields of large and viable seeds. This review summarizes and critically discusses results from over 30 years of research on garlic reproductive biology, its sterility and reported cases of fertility restoration, and the approaches used for true seed production in the species. Genetic, physiological and morphological factors affecting this trait are discussed. Unlocking garlic's sexual reproduction would have a tremendous positive impact in breeding, genetic research, and—ultimately—domestication of this flavourful vegetable. EEA Mendoza Fil: Dhall, Rajinder Kumar. Punjab Agricultural University. Department of Vegetable Science; India Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Fil: Cavagnaro, Pablo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cavagnaro, Pablo Federico. University of Agriculture in Krakow. Faculty of Biotechnology and Horticulture. Department of Plant Biology, and Biotechnology; Polonia Fil: Malik, Geetika. ICAR-Central Institute of Temperate Horticulture. Department of Vegetable Science and Floriculture; India Fil: Malik, Ajaz Ahmed. Sher-e- Kashmir University of Agricultural Science and Technology-Kashmir. Division of Vegetable Science; India Fil: Rana, Neha. Punjab Agricultural University. Department of Vegetable Science; India 2025-09-12T13:48:56Z 2025-09-12T13:48:56Z 2025-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23796 https://onlinelibrary.wiley.com/doi/10.1111/pbr.70012 0179-9541 1439-0523 https://doi.org/10.1111/pbr.70012 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Plant Breeding : 1-24. (First published: 05 August 2025)
spellingShingle Ajo
Fitomejoramiento
Expresión Génica
Floración
Transcriptoma
Garlic
Plant Breeding
Gene Expression
Flowering
Transcriptome
Allium sativum
Plant Biotechnology
Biotecnología Vegetal
Dhall, Rajinder Kumar
Cavagnaro, Pablo
Malik, Geetika
Malik, Ajaz Ahmed
Rana, Neha
Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review
title Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review
title_full Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review
title_fullStr Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review
title_full_unstemmed Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review
title_short Reproductive Biology, Sterility and Fertility Restoration, Breeding and Biotechnological Advances in Garlic: A Comprehensive Review
title_sort reproductive biology sterility and fertility restoration breeding and biotechnological advances in garlic a comprehensive review
topic Ajo
Fitomejoramiento
Expresión Génica
Floración
Transcriptoma
Garlic
Plant Breeding
Gene Expression
Flowering
Transcriptome
Allium sativum
Plant Biotechnology
Biotecnología Vegetal
url http://hdl.handle.net/20.500.12123/23796
https://onlinelibrary.wiley.com/doi/10.1111/pbr.70012
https://doi.org/10.1111/pbr.70012
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AT cavagnaropablo reproductivebiologysterilityandfertilityrestorationbreedingandbiotechnologicaladvancesingarlicacomprehensivereview
AT malikgeetika reproductivebiologysterilityandfertilityrestorationbreedingandbiotechnologicaladvancesingarlicacomprehensivereview
AT malikajazahmed reproductivebiologysterilityandfertilityrestorationbreedingandbiotechnologicaladvancesingarlicacomprehensivereview
AT rananeha reproductivebiologysterilityandfertilityrestorationbreedingandbiotechnologicaladvancesingarlicacomprehensivereview