Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system

A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNAHis an...

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Autores principales: Lencina, Franco Daniel, Prina, Alberto Raul, Pacheco, Maria Gabriela, Kobayashi, Ken, Landau, Alejandra Mabel
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: MDPI 2025
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/23777
https://www.mdpi.com/1422-0067/26/17/8644
https://doi.org/10.3390/ijms26178644
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author Lencina, Franco Daniel
Prina, Alberto Raul
Pacheco, Maria Gabriela
Kobayashi, Ken
Landau, Alejandra Mabel
author_browse Kobayashi, Ken
Landau, Alejandra Mabel
Lencina, Franco Daniel
Pacheco, Maria Gabriela
Prina, Alberto Raul
author_facet Lencina, Franco Daniel
Prina, Alberto Raul
Pacheco, Maria Gabriela
Kobayashi, Ken
Landau, Alejandra Mabel
author_sort Lencina, Franco Daniel
collection INTA Digital
description A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNAHis and rps19 genes, a 620 bp deletion in the psbA gene, a 79 bp deletion in the intergenic region between rpl33 and rps18 genes and a 45 bp deletion in the rps3 gene. The present investigation aims to understand the mechanisms producing the large indels and to better characterize the cpm mutagenic effect. Whole plastome sequencing revealed novel polymorphisms that were identified either in regions not previously examined or in regions that were explored but not detected through celery juice extract (CJE) digestion. The 620 bp deletion in the psbA gene was lethal when homoplastomic, whereas the 45 bp deletion in the rps3 gene did not affect the viability of the seedlings even in homoplastomy. The presence of direct repeats at the borders of large indels suggests that they could have originated by illegitimate recombination because of CPM protein malfunction. A truncated mismatch repair MSH1 protein identified in cpm seedlings suggests that CPM is involved in organellar genome stability maintenance.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
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spelling INTA237772025-09-11T13:26:47Z Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system Lencina, Franco Daniel Prina, Alberto Raul Pacheco, Maria Gabriela Kobayashi, Ken Landau, Alejandra Mabel Chloroplast DNA Cytoplasmic Organelles Barley Mutation ADN Cloroplástico Orgánulo Citoplasmico Cebada Mutación Plastome Plastoma A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNAHis and rps19 genes, a 620 bp deletion in the psbA gene, a 79 bp deletion in the intergenic region between rpl33 and rps18 genes and a 45 bp deletion in the rps3 gene. The present investigation aims to understand the mechanisms producing the large indels and to better characterize the cpm mutagenic effect. Whole plastome sequencing revealed novel polymorphisms that were identified either in regions not previously examined or in regions that were explored but not detected through celery juice extract (CJE) digestion. The 620 bp deletion in the psbA gene was lethal when homoplastomic, whereas the 45 bp deletion in the rps3 gene did not affect the viability of the seedlings even in homoplastomy. The presence of direct repeats at the borders of large indels suggests that they could have originated by illegitimate recombination because of CPM protein malfunction. A truncated mismatch repair MSH1 protein identified in cpm seedlings suggests that CPM is involved in organellar genome stability maintenance. Instituto de Genética Fil: Lencina, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Kobayashi, Ken. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2025-09-11T13:16:37Z 2025-09-11T13:16:37Z 2025-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23777 https://www.mdpi.com/1422-0067/26/17/8644 1422-0067 https://doi.org/10.3390/ijms26178644 eng info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001, Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repograntAgreement/INTA/PNBIO/1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI International Journal of Molecular Sciences 26 (17) : 8644 (September 2025)
spellingShingle Chloroplast DNA
Cytoplasmic Organelles
Barley
Mutation
ADN Cloroplástico
Orgánulo Citoplasmico
Cebada
Mutación
Plastome
Plastoma
Lencina, Franco Daniel
Prina, Alberto Raul
Pacheco, Maria Gabriela
Kobayashi, Ken
Landau, Alejandra Mabel
Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system
title Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system
title_full Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system
title_fullStr Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system
title_full_unstemmed Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system
title_short Four large indels in barley chloroplast mutator (cpm) seedlings reinforce the hypothesis of a malfunction in the MMR system
title_sort four large indels in barley chloroplast mutator cpm seedlings reinforce the hypothesis of a malfunction in the mmr system
topic Chloroplast DNA
Cytoplasmic Organelles
Barley
Mutation
ADN Cloroplástico
Orgánulo Citoplasmico
Cebada
Mutación
Plastome
Plastoma
url http://hdl.handle.net/20.500.12123/23777
https://www.mdpi.com/1422-0067/26/17/8644
https://doi.org/10.3390/ijms26178644
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