Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis)
Buffaloes are compulsory vaccinated against foot-and-mouth disease virus (FMDV) in many countries as part of the official control programmes. Serological testing aimed to indirectly assess herd immunity is currently performed using the same Enzyme-linked immunosorbent assays (ELISAs) applied for bov...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/2374 https://www.tandfonline.com/doi/10.1080/09712119.2017.1335641 https://doi.org/10.1080/09712119.2017.1335641 |
| _version_ | 1855034951688257536 |
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| author | Sala, Juan Manuel Trotta, Myrian Vanesa Mansilla, Florencia Celeste Perez Filgueira, Daniel Mariano Caspe, Sergio Gaston Capozzo, Alejandra |
| author_browse | Capozzo, Alejandra Caspe, Sergio Gaston Mansilla, Florencia Celeste Perez Filgueira, Daniel Mariano Sala, Juan Manuel Trotta, Myrian Vanesa |
| author_facet | Sala, Juan Manuel Trotta, Myrian Vanesa Mansilla, Florencia Celeste Perez Filgueira, Daniel Mariano Caspe, Sergio Gaston Capozzo, Alejandra |
| author_sort | Sala, Juan Manuel |
| collection | INTA Digital |
| description | Buffaloes are compulsory vaccinated against foot-and-mouth disease virus (FMDV) in many countries as part of the official control programmes. Serological testing aimed to indirectly assess herd immunity is currently performed using the same Enzyme-linked immunosorbent assays (ELISAs) applied for bovine sera, assuming an agreement between the ELISA’s diagnostic results and those obtained using the virus neutralization test (VNT). Here we evaluated the accuracy of different ELISA tests to assess vaccine-induced antibodies against FMDV in buffalo’s sera classified according to their VNT titres. Currently used liquid-phase blocking ELISA yielded very low specificity, producing high titres for many samples with low VNT titres. To increase specificity, we developed an indirect ELISA using purified 140S viral particles and an avidity single-dilution ELISA, which includes a urea washing step after the incubation of the diluted serum sample, to detach weak binders. Combining these two high-throughput single-dilution tests, an excellent concordance with VNT was achieved. This is the first study analysing the diagnostic agreement of traditional and novel serological tests with VNT for the indirect assessment of antibodies against FMDV capsid proteins in buffalo serum samples. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2374 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA23742024-04-15T11:22:42Z Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis) Sala, Juan Manuel Trotta, Myrian Vanesa Mansilla, Florencia Celeste Perez Filgueira, Daniel Mariano Caspe, Sergio Gaston Capozzo, Alejandra Búfalo de Agua Enfermedades de los Animales Fiebre Aftosa Vacuna Inmunidad Water Buffaloes Animal Diseases Foot and Mouth Disease Vaccines Immunity Buffaloes are compulsory vaccinated against foot-and-mouth disease virus (FMDV) in many countries as part of the official control programmes. Serological testing aimed to indirectly assess herd immunity is currently performed using the same Enzyme-linked immunosorbent assays (ELISAs) applied for bovine sera, assuming an agreement between the ELISA’s diagnostic results and those obtained using the virus neutralization test (VNT). Here we evaluated the accuracy of different ELISA tests to assess vaccine-induced antibodies against FMDV in buffalo’s sera classified according to their VNT titres. Currently used liquid-phase blocking ELISA yielded very low specificity, producing high titres for many samples with low VNT titres. To increase specificity, we developed an indirect ELISA using purified 140S viral particles and an avidity single-dilution ELISA, which includes a urea washing step after the incubation of the diluted serum sample, to detach weak binders. Combining these two high-throughput single-dilution tests, an excellent concordance with VNT was achieved. This is the first study analysing the diagnostic agreement of traditional and novel serological tests with VNT for the indirect assessment of antibodies against FMDV capsid proteins in buffalo serum samples. EEA Mercedes Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Trotta, Myrian Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-05-10T15:03:14Z 2018-05-10T15:03:14Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2374 https://www.tandfonline.com/doi/10.1080/09712119.2017.1335641 0971-2119 0974-1844 https://doi.org/10.1080/09712119.2017.1335641 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Applied Animal Research 46 (1) : 451-458. (2018) |
| spellingShingle | Búfalo de Agua Enfermedades de los Animales Fiebre Aftosa Vacuna Inmunidad Water Buffaloes Animal Diseases Foot and Mouth Disease Vaccines Immunity Sala, Juan Manuel Trotta, Myrian Vanesa Mansilla, Florencia Celeste Perez Filgueira, Daniel Mariano Caspe, Sergio Gaston Capozzo, Alejandra Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis) |
| title | Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis) |
| title_full | Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis) |
| title_fullStr | Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis) |
| title_full_unstemmed | Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis) |
| title_short | Alternatives for the serological assessment of foot-and-mouth disease vaccine immunity in buffaloes (Bubalus bubalis) |
| title_sort | alternatives for the serological assessment of foot and mouth disease vaccine immunity in buffaloes bubalus bubalis |
| topic | Búfalo de Agua Enfermedades de los Animales Fiebre Aftosa Vacuna Inmunidad Water Buffaloes Animal Diseases Foot and Mouth Disease Vaccines Immunity |
| url | http://hdl.handle.net/20.500.12123/2374 https://www.tandfonline.com/doi/10.1080/09712119.2017.1335641 https://doi.org/10.1080/09712119.2017.1335641 |
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