Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum
Background, Context or Rationale Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection in humans may cause bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome (HUS). Cattle are the main reservoir of EHEC and a source of infection for humans. It has been previously demonstrat...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/23656 https://onlinelibrary.wiley.com/doi/10.1111/1471-0307.70045 https://doi.org/10.1111/1471-0307.70045 |
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| author | Girón, Daniel Marques Da Silva, Wanderson Vilte, Daniel Alejandro Smith, Libia Yael Casabonne, Maria Carolina Maldonado May, Veronica Cataldi, Angel Adrian Ibarra, Cristina Adriana Larzabal, Mariano Sacerdoti, Flavia |
| author_browse | Casabonne, Maria Carolina Cataldi, Angel Adrian Girón, Daniel Ibarra, Cristina Adriana Larzabal, Mariano Maldonado May, Veronica Marques Da Silva, Wanderson Sacerdoti, Flavia Smith, Libia Yael Vilte, Daniel Alejandro |
| author_facet | Girón, Daniel Marques Da Silva, Wanderson Vilte, Daniel Alejandro Smith, Libia Yael Casabonne, Maria Carolina Maldonado May, Veronica Cataldi, Angel Adrian Ibarra, Cristina Adriana Larzabal, Mariano Sacerdoti, Flavia |
| author_sort | Girón, Daniel |
| collection | INTA Digital |
| description | Background, Context or Rationale
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection in humans may cause bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome (HUS). Cattle are the main reservoir of EHEC and a source of infection for humans. It has been previously demonstrated that hyperimmune bovine colostrum (HC) against virulence factors of EHEC was efficiently transferred to newborn calves through lactation. Also, it is well described that human consumption of HC can provide therapeutic effects on several gastrointestinal infections.
Aim(s)
The objectives of the present work were to produce HC using two recombinant virulence factors of EHEC as immunogen: C-terminal fragment of Intimin (C280-Int) and Shiga toxin type 2 (Stx2), and then to evaluate specific IgG levels and correlate them with the ability of HC to neutralise the cytotoxic activity of Stx2 and EHEC O157:H7 adhesion.
Methods
For this, 12 pregnant Aberdeen Angus breed cows were immunised with two doses of C280-Int and Stx2 at 40 and 20 days before calving. Colostrum was obtained within 24 h after delivery and specific IgG against Stx2 and Intimin was evaluated by ELISA. Stx2 neutralisation capacity of HC was evaluated on Vero cells. EHEC O157:H7 adherence inhibition was evaluated on human intestinal epithelial cells (HCT-8).
Major Findings
Stx2 neutraliation capacity was observed in nine of the HC obtained, and 10 colostrum could inhibit adherence of EHEC O157:H7 to HCT-8 cells. A correlation was observed between Stx2 neutralisation and adherence inhibition of EHEC with specific IgG levels thus indicating that specific IgG levels may predict the neutralisation capacity of colostrum.
Scientific or Industrial Implications
HC against EHEC virulence factors is effective in neutralising the virulence activity of EHEC O157:H7. This opens a perspective on the utilisation of HC for controlling EHEC colonisation in calves and to prevent the development HUS in humans. |
| format | Artículo |
| id | INTA23656 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA236562025-09-02T13:39:19Z Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum Girón, Daniel Marques Da Silva, Wanderson Vilte, Daniel Alejandro Smith, Libia Yael Casabonne, Maria Carolina Maldonado May, Veronica Cataldi, Angel Adrian Ibarra, Cristina Adriana Larzabal, Mariano Sacerdoti, Flavia Antibodies Colostrum Neutralization Virulence Anticuerpos Calostro Neutralización Escherichia coli Virulencia Background, Context or Rationale Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection in humans may cause bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome (HUS). Cattle are the main reservoir of EHEC and a source of infection for humans. It has been previously demonstrated that hyperimmune bovine colostrum (HC) against virulence factors of EHEC was efficiently transferred to newborn calves through lactation. Also, it is well described that human consumption of HC can provide therapeutic effects on several gastrointestinal infections. Aim(s) The objectives of the present work were to produce HC using two recombinant virulence factors of EHEC as immunogen: C-terminal fragment of Intimin (C280-Int) and Shiga toxin type 2 (Stx2), and then to evaluate specific IgG levels and correlate them with the ability of HC to neutralise the cytotoxic activity of Stx2 and EHEC O157:H7 adhesion. Methods For this, 12 pregnant Aberdeen Angus breed cows were immunised with two doses of C280-Int and Stx2 at 40 and 20 days before calving. Colostrum was obtained within 24 h after delivery and specific IgG against Stx2 and Intimin was evaluated by ELISA. Stx2 neutralisation capacity of HC was evaluated on Vero cells. EHEC O157:H7 adherence inhibition was evaluated on human intestinal epithelial cells (HCT-8). Major Findings Stx2 neutraliation capacity was observed in nine of the HC obtained, and 10 colostrum could inhibit adherence of EHEC O157:H7 to HCT-8 cells. A correlation was observed between Stx2 neutralisation and adherence inhibition of EHEC with specific IgG levels thus indicating that specific IgG levels may predict the neutralisation capacity of colostrum. Scientific or Industrial Implications HC against EHEC virulence factors is effective in neutralising the virulence activity of EHEC O157:H7. This opens a perspective on the utilisation of HC for controlling EHEC colonisation in calves and to prevent the development HUS in humans. Instituto de Biotecnología Fil: Girón, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Médicas. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina Fil: Girón, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Médicas. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Girón, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Vilte, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Casabonne, María C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Casabonne, María C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maldonado May, Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Maldonado May, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Ciencias Médicas. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sacerdoti, Flavia. Universidad de Buenos Aires. Facultad de Ciencias Médicas. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina Fil: Sacerdoti, Flavia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-09-02T13:25:39Z 2025-09-02T13:25:39Z 2025-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23656 https://onlinelibrary.wiley.com/doi/10.1111/1471-0307.70045 1471-0307 https://doi.org/10.1111/1471-0307.70045 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley International Journal of Dairy Technology 78 (3) : e70045 (July–September 2025) |
| spellingShingle | Antibodies Colostrum Neutralization Virulence Anticuerpos Calostro Neutralización Escherichia coli Virulencia Girón, Daniel Marques Da Silva, Wanderson Vilte, Daniel Alejandro Smith, Libia Yael Casabonne, Maria Carolina Maldonado May, Veronica Cataldi, Angel Adrian Ibarra, Cristina Adriana Larzabal, Mariano Sacerdoti, Flavia Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum |
| title | Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum |
| title_full | Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum |
| title_fullStr | Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum |
| title_full_unstemmed | Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum |
| title_short | Neutralisation of virulence activity of enterohemorrhagic Escherichia coli O157:H7 in vitro through hyperimmune bovine colostrum |
| title_sort | neutralisation of virulence activity of enterohemorrhagic escherichia coli o157 h7 in vitro through hyperimmune bovine colostrum |
| topic | Antibodies Colostrum Neutralization Virulence Anticuerpos Calostro Neutralización Escherichia coli Virulencia |
| url | http://hdl.handle.net/20.500.12123/23656 https://onlinelibrary.wiley.com/doi/10.1111/1471-0307.70045 https://doi.org/10.1111/1471-0307.70045 |
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