Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
The cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/23644 https://www.sciencedirect.com/science/article/pii/S1877959X2500086X https://doi.org/10.1016/j.ttbdis.2025.102522 |
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| author | Pérez, Agustina Ericlee Guillemi, Eliana Carolina Abuin-Denis, Lianet Piloto-Sardiñas, Elianne Obregón, Dasiel Pin Viso, Natalia Daniela Sarmiento, Nestor Fabian Cabezas-Cruz, Alejandro Farber, Marisa Diana |
| author_browse | Abuin-Denis, Lianet Cabezas-Cruz, Alejandro Farber, Marisa Diana Guillemi, Eliana Carolina Obregón, Dasiel Piloto-Sardiñas, Elianne Pin Viso, Natalia Daniela Pérez, Agustina Ericlee Sarmiento, Nestor Fabian |
| author_facet | Pérez, Agustina Ericlee Guillemi, Eliana Carolina Abuin-Denis, Lianet Piloto-Sardiñas, Elianne Obregón, Dasiel Pin Viso, Natalia Daniela Sarmiento, Nestor Fabian Cabezas-Cruz, Alejandro Farber, Marisa Diana |
| author_sort | Pérez, Agustina Ericlee |
| collection | INTA Digital |
| description | The cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how A. marginale infection affects the bacterial community within the salivary glands and ovaries of R. microplus. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with A. marginale by targeting the msp1β gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the 16S rRNA V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. Sphingomonadaceae was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that A. marginale infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that A. marginale impacts the microbiota of R. microplus at an organ-specific level. |
| format | Artículo |
| id | INTA23644 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA236442025-09-02T10:16:16Z Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission Pérez, Agustina Ericlee Guillemi, Eliana Carolina Abuin-Denis, Lianet Piloto-Sardiñas, Elianne Obregón, Dasiel Pin Viso, Natalia Daniela Sarmiento, Nestor Fabian Cabezas-Cruz, Alejandro Farber, Marisa Diana Anaplasma marginale Microbiomes Ovaries Pathogens Rhipicephalus Salivary Glands Microbiomas Ovarios Organismos Patógenos Glándulas Salivales Rhipicephalus microplus The cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how A. marginale infection affects the bacterial community within the salivary glands and ovaries of R. microplus. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with A. marginale by targeting the msp1β gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the 16S rRNA V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. Sphingomonadaceae was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that A. marginale infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that A. marginale impacts the microbiota of R. microplus at an organ-specific level. Instituto de Biotecnología Fil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Abuin-Denis, Lianet. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia Fil: Abuin-Denis, Lianet. Center for Genetic Engineering and Biotechnology. Animal Biotechnology Department; Cuba Fil: Piloto-Sardiñas, Elianne. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia Fil: Piloto-Sardiñas, Elianne. National Center for Animal and Plant Health. Direction of Animal Health; Cuba Fil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; Canadá Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Cabezas-Cruz, Alejandro. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia Fil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Farber, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-09-02T10:08:58Z 2025-09-02T10:08:58Z 2025-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23644 https://www.sciencedirect.com/science/article/pii/S1877959X2500086X 1877-959X https://doi.org/10.1016/j.ttbdis.2025.102522 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Ticks and Tick-borne Diseases 16 (5) : 102522 (September 2025) |
| spellingShingle | Anaplasma marginale Microbiomes Ovaries Pathogens Rhipicephalus Salivary Glands Microbiomas Ovarios Organismos Patógenos Glándulas Salivales Rhipicephalus microplus Pérez, Agustina Ericlee Guillemi, Eliana Carolina Abuin-Denis, Lianet Piloto-Sardiñas, Elianne Obregón, Dasiel Pin Viso, Natalia Daniela Sarmiento, Nestor Fabian Cabezas-Cruz, Alejandro Farber, Marisa Diana Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission |
| title | Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission |
| title_full | Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission |
| title_fullStr | Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission |
| title_full_unstemmed | Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission |
| title_short | Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission |
| title_sort | anaplasma marginale modulates the microbiota of rhipicephalus microplus organs involved in pathogen transmission |
| topic | Anaplasma marginale Microbiomes Ovaries Pathogens Rhipicephalus Salivary Glands Microbiomas Ovarios Organismos Patógenos Glándulas Salivales Rhipicephalus microplus |
| url | http://hdl.handle.net/20.500.12123/23644 https://www.sciencedirect.com/science/article/pii/S1877959X2500086X https://doi.org/10.1016/j.ttbdis.2025.102522 |
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