Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation expe...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2360 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691 http://dx.doi.org/10.1590/0001-3765201520130110 |
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| author | Mondino, Eduardo Ariel Covacevich, Fernanda Studdert, Guillermo Pimentel, João Pedro Berbara, Ricardo Luiz Louro |
| author_browse | Berbara, Ricardo Luiz Louro Covacevich, Fernanda Mondino, Eduardo Ariel Pimentel, João Pedro Studdert, Guillermo |
| author_facet | Mondino, Eduardo Ariel Covacevich, Fernanda Studdert, Guillermo Pimentel, João Pedro Berbara, Ricardo Luiz Louro |
| author_sort | Mondino, Eduardo Ariel |
| collection | INTA Digital |
| description | We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and
cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of
nematodes. Nematodes were isolated from an agricultural area under different management of long-term
crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain
Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The
CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful
method because positive amplifications were obtained by using both eukaryotic and nematode specific
primers. This work could contribute to biodiversity studies of nematodes on agroecosystems. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2360 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA23602018-05-14T12:17:55Z Extracting DNA of nematodes communities from Argentine Pampas agricultural soils Mondino, Eduardo Ariel Covacevich, Fernanda Studdert, Guillermo Pimentel, João Pedro Berbara, Ricardo Luiz Louro Suelos Agrícolas Nematoda ADN Agroecosistemas Agroecosystems DNA Agricultural Soils Región Pampeana Cetyltrimethylammonium Bromide CTAB We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems. Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; Brasil Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina Fil: Studdert, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Pimentel, João Pedro. Universidade Federal Rural do Rio de Janeiro. Departamento de Entomologia e Fitopatologia. Instituto de Biologia; Brasil Fil: Berbara, Ricardo Luiz Louro Universidade Federal Rural do Rio de Janeiro. Instituto de Agronomia. Departamento de Solos. Laboratório de Matéria Orgânica e Biologia do Solo; Brasil 2018-05-09T14:37:28Z 2018-05-09T14:37:28Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2360 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691 0001-3765 1678-2690 (Online version) http://dx.doi.org/10.1590/0001-3765201520130110 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Anais da Academia Brasileira de Ciências 87 (2) : 691-697. (2015) |
| spellingShingle | Suelos Agrícolas Nematoda ADN Agroecosistemas Agroecosystems DNA Agricultural Soils Región Pampeana Cetyltrimethylammonium Bromide CTAB Mondino, Eduardo Ariel Covacevich, Fernanda Studdert, Guillermo Pimentel, João Pedro Berbara, Ricardo Luiz Louro Extracting DNA of nematodes communities from Argentine Pampas agricultural soils |
| title | Extracting DNA of nematodes communities from Argentine Pampas agricultural soils |
| title_full | Extracting DNA of nematodes communities from Argentine Pampas agricultural soils |
| title_fullStr | Extracting DNA of nematodes communities from Argentine Pampas agricultural soils |
| title_full_unstemmed | Extracting DNA of nematodes communities from Argentine Pampas agricultural soils |
| title_short | Extracting DNA of nematodes communities from Argentine Pampas agricultural soils |
| title_sort | extracting dna of nematodes communities from argentine pampas agricultural soils |
| topic | Suelos Agrícolas Nematoda ADN Agroecosistemas Agroecosystems DNA Agricultural Soils Región Pampeana Cetyltrimethylammonium Bromide CTAB |
| url | http://hdl.handle.net/20.500.12123/2360 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691 http://dx.doi.org/10.1590/0001-3765201520130110 |
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