FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
We present here the physical mapping of the 5S rDNA locus in six wild and fi ve cultivated taxa of Capsicum by means of a genus-specifi c FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2350 http://dx.doi.org/10.1590/0001-37652301620140616 |
| _version_ | 1855483039392464896 |
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| author | Aguilera, Patricia Mabel Debat, Humberto Julio Scaldaferro, Marisel Analia Marti, Dardo Andrea Grabiele, Mauro |
| author_browse | Aguilera, Patricia Mabel Debat, Humberto Julio Grabiele, Mauro Marti, Dardo Andrea Scaldaferro, Marisel Analia |
| author_facet | Aguilera, Patricia Mabel Debat, Humberto Julio Scaldaferro, Marisel Analia Marti, Dardo Andrea Grabiele, Mauro |
| author_sort | Aguilera, Patricia Mabel |
| collection | INTA Digital |
| description | We present here the physical mapping of the 5S rDNA locus in six wild and fi ve cultivated taxa of Capsicum
by means of a genus-specifi c FISH probe. In all taxa, a single 5S locus per haploid genome that persistently
mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S
FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This
is the fi rst cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe,
and the most exhaustive and comprehensive in the chili peppers up to now. The information provided
here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be
instrumental to integrate physical, genetic and genomic maps already generated in the genus. |
| format | Artículo |
| id | INTA2350 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA23502018-05-08T14:27:02Z FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) Aguilera, Patricia Mabel Debat, Humberto Julio Scaldaferro, Marisel Analia Marti, Dardo Andrea Grabiele, Mauro Capsicum Espectroscopia Emisión Fluorescente Genes Fluorescence Emission Spectroscopy 5S rRNA Gene Fluorescence In Situ Hybridization FISH Mapping Pimiento We present here the physical mapping of the 5S rDNA locus in six wild and fi ve cultivated taxa of Capsicum by means of a genus-specifi c FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the fi rst cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus. Neste trabalho, apresentamos o mapeamento físico do locus 5S rDNA em seis taxa silvestres e cinco cultivados de Capsicum, obtido através do uso de uma sonda FISH gênero-específica. Em todos os taxa, um único locus 5S por genoma haplóide foi persistentemente mapeado no braço curto de um único par de cromossomos metacêntricos na posição intercalar, e foram observados sinais 5S de FISH de tamanho e intensidade de brilho quase semelhantes. Esta é a primeira caracterização citológica dos 5S nos taxa silvestres de Capsicum usando uma sonda gênero-específica e o estudo sobre o grupo das pimentas mais exaustivo e abrangente realizado até ao momento. A informação aqui fornecida irá ajudar a caracterização citomolecular do germoplasma das pimentas, permitindo avaliar a sua variabilidade e podendo ser usada para integrar mapas físicos, genéticos e genômicos já realizados no gênero. Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Scaldaferro, Marisel Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina 2018-05-08T14:20:03Z 2018-05-08T14:20:03Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2350 0001-3765 1678-2690 (Online version) http://dx.doi.org/10.1590/0001-37652301620140616 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Anais da Academia Brasileira de Ciências 88 (1) : 117-125. (2016) |
| spellingShingle | Capsicum Espectroscopia Emisión Fluorescente Genes Fluorescence Emission Spectroscopy 5S rRNA Gene Fluorescence In Situ Hybridization FISH Mapping Pimiento Aguilera, Patricia Mabel Debat, Humberto Julio Scaldaferro, Marisel Analia Marti, Dardo Andrea Grabiele, Mauro FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
| title | FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
| title_full | FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
| title_fullStr | FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
| title_full_unstemmed | FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
| title_short | FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
| title_sort | fish mapping of the 5s rdna locus in chili peppers capsicum solanaceae |
| topic | Capsicum Espectroscopia Emisión Fluorescente Genes Fluorescence Emission Spectroscopy 5S rRNA Gene Fluorescence In Situ Hybridization FISH Mapping Pimiento |
| url | http://hdl.handle.net/20.500.12123/2350 http://dx.doi.org/10.1590/0001-37652301620140616 |
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