Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis
Caprine brucellosis, caused by Brucella melitensis, is a zoonotic disease responsible for abortions in goats and is endemic in several countries. Control measures include vaccination, serological assays, and culling of infected animals. Sensitive, specific, automatable, and low-cost serological assa...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/23419 https://www.sciencedirect.com/science/article/abs/pii/S0165242725000510 https://doi.org/10.1016/j.vetimm.2025.110931 |
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| author | Foster, Camila Nayla Monzon, Nolly María Signorini Porchiett, Marcelo Lisandro Aznar, Maria Natalia Aguirre, Nerina Patricia Valentini, Beatriz Susana Vanzini, Víctor René Novoa, María Belén |
| author_browse | Aguirre, Nerina Patricia Aznar, Maria Natalia Foster, Camila Nayla Monzon, Nolly María Novoa, María Belén Signorini Porchiett, Marcelo Lisandro Valentini, Beatriz Susana Vanzini, Víctor René |
| author_facet | Foster, Camila Nayla Monzon, Nolly María Signorini Porchiett, Marcelo Lisandro Aznar, Maria Natalia Aguirre, Nerina Patricia Valentini, Beatriz Susana Vanzini, Víctor René Novoa, María Belén |
| author_sort | Foster, Camila Nayla |
| collection | INTA Digital |
| description | Caprine brucellosis, caused by Brucella melitensis, is a zoonotic disease responsible for abortions in goats and is endemic in several countries. Control measures include vaccination, serological assays, and culling of infected animals. Sensitive, specific, automatable, and low-cost serological assays are essential for use in endemic regions. This study aimed to develop and validate a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) to detect anti-B. melitensis antibodies in goats. The ciELISA was standardized with pre-dilutions of ISaBmS (1/8 and 1/300) in negative goat serum. Validation was done using 1254 serum samples from non-vaccinated goats: 843 from brucellosis-free flocks and 411 from infected flocks in Argentina. Serum samples were tested with the ciELISA, buffer plate antigen (BPA) test, fluorescence-polarized antigen (FPA) test, and complement fixation test (CFT). The optimal ciELISA cutoff was determined using a receiver operating characteristic (ROC) curve, with CFT as the gold standard. Sensitivity and specificity were evaluated using the Bayesian Latent Class Model (BLCM). The ciELISA showed sensitivity of 98.29 % (95 % CI: 96.4–99.6) and specificity of 98.62 % (95 % CI: 97.6–99.6). The kappa statistic (κ) values between ciELISA and other tests were: BPA (κ = 0.94), FPA (κ = 0.79), and CFT (κ = 0.95). The ciELISA demonstrated excellent performance, being rapid and objective, making it suitable for detecting anti-B. melitensis antibodies in goat serum. |
| format | Artículo |
| id | INTA23419 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA234192025-08-14T10:14:58Z Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis Foster, Camila Nayla Monzon, Nolly María Signorini Porchiett, Marcelo Lisandro Aznar, Maria Natalia Aguirre, Nerina Patricia Valentini, Beatriz Susana Vanzini, Víctor René Novoa, María Belén Brucella melitensis Brucelosis Anticuerpos Caprinos Brucellosis Antibodies ELISA Goats Bayesian Latent Class Analysis Análisis de Clases Latentes Bayesiano Caprine brucellosis, caused by Brucella melitensis, is a zoonotic disease responsible for abortions in goats and is endemic in several countries. Control measures include vaccination, serological assays, and culling of infected animals. Sensitive, specific, automatable, and low-cost serological assays are essential for use in endemic regions. This study aimed to develop and validate a competitive inhibition enzyme-linked immunosorbent assay (ciELISA) to detect anti-B. melitensis antibodies in goats. The ciELISA was standardized with pre-dilutions of ISaBmS (1/8 and 1/300) in negative goat serum. Validation was done using 1254 serum samples from non-vaccinated goats: 843 from brucellosis-free flocks and 411 from infected flocks in Argentina. Serum samples were tested with the ciELISA, buffer plate antigen (BPA) test, fluorescence-polarized antigen (FPA) test, and complement fixation test (CFT). The optimal ciELISA cutoff was determined using a receiver operating characteristic (ROC) curve, with CFT as the gold standard. Sensitivity and specificity were evaluated using the Bayesian Latent Class Model (BLCM). The ciELISA showed sensitivity of 98.29 % (95 % CI: 96.4–99.6) and specificity of 98.62 % (95 % CI: 97.6–99.6). The kappa statistic (κ) values between ciELISA and other tests were: BPA (κ = 0.94), FPA (κ = 0.79), and CFT (κ = 0.95). The ciELISA demonstrated excellent performance, being rapid and objective, making it suitable for detecting anti-B. melitensis antibodies in goat serum. Asociación Cooperadora INTA Rafaela. Laboratorio Brucelosis. Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (PICT 2021-INVI-809). EEA Rafaela Fil: Foster, Camila Nayla. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Foster, Camila Nayla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Monzon, Nolly María. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Aznar, María Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Aguirre, Nerina Patricia. Laboratorio Biovet. Rafaela, Santa Fe; Argentina Fil: Valentini, Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Valentini, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Vanzini, Víctor René. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Novoa, María Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina 2025-08-14T10:04:37Z 2025-08-14T10:04:37Z 2025-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23419 https://www.sciencedirect.com/science/article/abs/pii/S0165242725000510 0165-2427 1873-2534 (online) https://doi.org/10.1016/j.vetimm.2025.110931 eng info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinario info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Immunology and Immunopathology 283 : 110931 (May 2025) |
| spellingShingle | Brucella melitensis Brucelosis Anticuerpos Caprinos Brucellosis Antibodies ELISA Goats Bayesian Latent Class Analysis Análisis de Clases Latentes Bayesiano Foster, Camila Nayla Monzon, Nolly María Signorini Porchiett, Marcelo Lisandro Aznar, Maria Natalia Aguirre, Nerina Patricia Valentini, Beatriz Susana Vanzini, Víctor René Novoa, María Belén Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis |
| title | Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis |
| title_full | Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis |
| title_fullStr | Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis |
| title_full_unstemmed | Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis |
| title_short | Development and Bayesian analysis of a competitive inhibition ELISA for caprine brucellosis |
| title_sort | development and bayesian analysis of a competitive inhibition elisa for caprine brucellosis |
| topic | Brucella melitensis Brucelosis Anticuerpos Caprinos Brucellosis Antibodies ELISA Goats Bayesian Latent Class Analysis Análisis de Clases Latentes Bayesiano |
| url | http://hdl.handle.net/20.500.12123/23419 https://www.sciencedirect.com/science/article/abs/pii/S0165242725000510 https://doi.org/10.1016/j.vetimm.2025.110931 |
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