Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production
Bovine Viral Diarrhea Virus (BVDV) and anti-BVDV antibodies have been detected in ovarian follicular fluid of infected cows. The aim of this study was to assess both the frequency of BVDV detection and the prevalence of anti-BVDV antibodies in pooled follicular fluid obtained from slaughterhouse ova...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Español |
| Published: |
Elsevier
2025
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/23374 https://www.sciencedirect.com/science/article/abs/pii/S0093691X25003395 https://doi.org/10.1016/j.theriogenology.2025.117613 |
| _version_ | 1855038743172349952 |
|---|---|
| author | Yavorsky, Marisol Solange Norero, Natalia Sigrid Spetter, Lucas Maximiliano Pereyra, Susana Beatriz Verna, Andrea Elizabeth Urrutia Luna, Naiara Rios, Glenda Laura González Altamiranda, Erika |
| author_browse | González Altamiranda, Erika Norero, Natalia Sigrid Pereyra, Susana Beatriz Rios, Glenda Laura Spetter, Lucas Maximiliano Urrutia Luna, Naiara Verna, Andrea Elizabeth Yavorsky, Marisol Solange |
| author_facet | Yavorsky, Marisol Solange Norero, Natalia Sigrid Spetter, Lucas Maximiliano Pereyra, Susana Beatriz Verna, Andrea Elizabeth Urrutia Luna, Naiara Rios, Glenda Laura González Altamiranda, Erika |
| author_sort | Yavorsky, Marisol Solange |
| collection | INTA Digital |
| description | Bovine Viral Diarrhea Virus (BVDV) and anti-BVDV antibodies have been detected in ovarian follicular fluid of infected cows. The aim of this study was to assess both the frequency of BVDV detection and the prevalence of anti-BVDV antibodies in pooled follicular fluid obtained from slaughterhouse ovaries for in vitro embryo production. Additionally, we sought to determine whether specific antibodies present in these samples are able to neutralize different BVDV strains. BVDV RNA was detected by using reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), while viral viability was evaluated by using viral isolation (VI). Antibody titers against different BVDV strains (reference strain NADL-1a Vs145; field strain 13/558-1a; field strains 10/49-1b) were determined through viral neutralization (VN) assays. A total of 60 pooled follicular fluid samples from slaughterhouse ovaries were analyzed over a period of 19 months. Results showed that 45 % (27 out of 60) of the samples tested positive for BVDV RNA via RT-PCR and RT-qPCR, although no samples yielded viable virus through the VI technique. The seroprevalence of anti-BVDV antibodies was 98 % (59 out of 60), with antibody titers sufficient (1:136 and 1:268) to neutralize the field strains tested. These findings indicate a high prevalence of BVDV RNA and extensive seroprevalence of anti-BVDV antibodies in pooled follicular fluid samples. The high antibody levels may compromise the sensitivity of VI, likely due to a viral inhibitory effect. Such inhibition could also influence the dynamics of infection throughout the in vitro embryo production process. |
| format | info:ar-repo/semantics/artículo |
| id | INTA23374 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Español |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA233742025-08-08T13:37:39Z Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production Yavorsky, Marisol Solange Norero, Natalia Sigrid Spetter, Lucas Maximiliano Pereyra, Susana Beatriz Verna, Andrea Elizabeth Urrutia Luna, Naiara Rios, Glenda Laura González Altamiranda, Erika Pestivirus de la diarrea bovina Bovine diarrhoea pestivirus Bovinae Anticuerpo Antibodies ARN RNA Producción de embriones in vitro In vitro embryo production Ovario Ovaries Enfermedad de los animales Animal diseases Bovine Viral Diarrhea Virus (BVDV) and anti-BVDV antibodies have been detected in ovarian follicular fluid of infected cows. The aim of this study was to assess both the frequency of BVDV detection and the prevalence of anti-BVDV antibodies in pooled follicular fluid obtained from slaughterhouse ovaries for in vitro embryo production. Additionally, we sought to determine whether specific antibodies present in these samples are able to neutralize different BVDV strains. BVDV RNA was detected by using reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), while viral viability was evaluated by using viral isolation (VI). Antibody titers against different BVDV strains (reference strain NADL-1a Vs145; field strain 13/558-1a; field strains 10/49-1b) were determined through viral neutralization (VN) assays. A total of 60 pooled follicular fluid samples from slaughterhouse ovaries were analyzed over a period of 19 months. Results showed that 45 % (27 out of 60) of the samples tested positive for BVDV RNA via RT-PCR and RT-qPCR, although no samples yielded viable virus through the VI technique. The seroprevalence of anti-BVDV antibodies was 98 % (59 out of 60), with antibody titers sufficient (1:136 and 1:268) to neutralize the field strains tested. These findings indicate a high prevalence of BVDV RNA and extensive seroprevalence of anti-BVDV antibodies in pooled follicular fluid samples. The high antibody levels may compromise the sensitivity of VI, likely due to a viral inhibitory effect. Such inhibition could also influence the dynamics of infection throughout the in vitro embryo production process. EEA Balcarce Fil: Yavorsky, Marisol Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Norero, Natalia Sigrid. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Spetter, Maximiliano Joaquín. New Mexico State University. Department of Animal and Range Sciences; Estados Unidos Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Urrutia Luna, Naiara. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: Ríos, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina Fil: González Altamiranda, Erika Analía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina 2025-08-08T13:27:50Z 2025-08-08T13:27:50Z 2025-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23374 https://www.sciencedirect.com/science/article/abs/pii/S0093691X25003395 0093-691X (print) 1879-3231 (online) https://doi.org/10.1016/j.theriogenology.2025.117613 spa info:eu-repograntAgreement/INTA/2023-PD-L01-I112, Biotecnologías reproductivas y plataforma de edición génica para animales de interés zootécnico. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Theriogenology 248, 117613 (December 2025) |
| spellingShingle | Pestivirus de la diarrea bovina Bovine diarrhoea pestivirus Bovinae Anticuerpo Antibodies ARN RNA Producción de embriones in vitro In vitro embryo production Ovario Ovaries Enfermedad de los animales Animal diseases Yavorsky, Marisol Solange Norero, Natalia Sigrid Spetter, Lucas Maximiliano Pereyra, Susana Beatriz Verna, Andrea Elizabeth Urrutia Luna, Naiara Rios, Glenda Laura González Altamiranda, Erika Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production |
| title | Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production |
| title_full | Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production |
| title_fullStr | Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production |
| title_full_unstemmed | Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production |
| title_short | Detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid: A potential risk for in vitro embryo production |
| title_sort | detection and prevalence of bovine viral diarrhea virus in ovarian follicular fluid a potential risk for in vitro embryo production |
| topic | Pestivirus de la diarrea bovina Bovine diarrhoea pestivirus Bovinae Anticuerpo Antibodies ARN RNA Producción de embriones in vitro In vitro embryo production Ovario Ovaries Enfermedad de los animales Animal diseases |
| url | http://hdl.handle.net/20.500.12123/23374 https://www.sciencedirect.com/science/article/abs/pii/S0093691X25003395 https://doi.org/10.1016/j.theriogenology.2025.117613 |
| work_keys_str_mv | AT yavorskymarisolsolange detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction AT noreronataliasigrid detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction AT spetterlucasmaximiliano detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction AT pereyrasusanabeatriz detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction AT vernaandreaelizabeth detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction AT urrutialunanaiara detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction AT riosglendalaura detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction AT gonzalezaltamirandaerika detectionandprevalenceofbovineviraldiarrheavirusinovarianfollicularfluidapotentialriskforinvitroembryoproduction |