Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
Neospora caninum infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the N. caninum NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegati...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s00436-013-3424-1 http://hdl.handle.net/20.500.12123/2329 https://doi.org/10.1007/s00436-013-3424-1 |
| _version_ | 1855034943210520576 |
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| author | Bacigalupe, Diana Basso, W. Caspe, Sergio Gaston More, Gastón Lischinsky, Lilian Haydee Gos, María Laura Leunda, Maria Rosa Campero, Lucía María Moore, Prando Dadin Schares, Gereon Campero, Carlos Manuel Venturini, María Cecilia |
| author_browse | Bacigalupe, Diana Basso, W. Campero, Carlos Manuel Campero, Lucía María Caspe, Sergio Gaston Gos, María Laura Leunda, Maria Rosa Lischinsky, Lilian Haydee Moore, Prando Dadin More, Gastón Schares, Gereon Venturini, María Cecilia |
| author_facet | Bacigalupe, Diana Basso, W. Caspe, Sergio Gaston More, Gastón Lischinsky, Lilian Haydee Gos, María Laura Leunda, Maria Rosa Campero, Lucía María Moore, Prando Dadin Schares, Gereon Campero, Carlos Manuel Venturini, María Cecilia |
| author_sort | Bacigalupe, Diana |
| collection | INTA Digital |
| description | Neospora caninum infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the N. caninum NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to N. caninum were intravenously inoculated with tachyzoites of the NC-6 Argentina N. caninum strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for N. caninum antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for N. caninum DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for N. caninum. Inoculated animals had significantly higher N. caninum antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the N. caninum DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2329 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA23292021-12-27T13:51:45Z Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams Bacigalupe, Diana Basso, W. Caspe, Sergio Gaston More, Gastón Lischinsky, Lilian Haydee Gos, María Laura Leunda, Maria Rosa Campero, Lucía María Moore, Prando Dadin Schares, Gereon Campero, Carlos Manuel Venturini, María Cecilia Neospora caninum Ganado Bovino Aborto Técnicas Inmunológicas Inoculación Respuesta Inmunológica Cattle Abortion Immunological Techniques Inoculation Immune Response Argentina Neospora caninum infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the N. caninum NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to N. caninum were intravenously inoculated with tachyzoites of the NC-6 Argentina N. caninum strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for N. caninum antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for N. caninum DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for N. caninum. Inoculated animals had significantly higher N. caninum antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the N. caninum DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams. EEA Mercedes EEA Balcarce Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Basso, W. University of Zurich. Vetsuisse Faculty. Institute of Parasitology; Suiza Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: More, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lischinsky, Lilian Haydee. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Gos, M.L. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schares, Gereon. Friedrich-Loeffler-Institut. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Venturini, María C. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina 2018-05-04T17:28:19Z 2018-05-04T17:28:19Z 2013-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00436-013-3424-1 http://hdl.handle.net/20.500.12123/2329 0932-0113 1432-1955 https://doi.org/10.1007/s00436-013-3424-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Parasitology research 112 (7) : 2585–2592. (July 2013) |
| spellingShingle | Neospora caninum Ganado Bovino Aborto Técnicas Inmunológicas Inoculación Respuesta Inmunológica Cattle Abortion Immunological Techniques Inoculation Immune Response Argentina Bacigalupe, Diana Basso, W. Caspe, Sergio Gaston More, Gastón Lischinsky, Lilian Haydee Gos, María Laura Leunda, Maria Rosa Campero, Lucía María Moore, Prando Dadin Schares, Gereon Campero, Carlos Manuel Venturini, María Cecilia Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams |
| title | Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams |
| title_full | Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams |
| title_fullStr | Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams |
| title_full_unstemmed | Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams |
| title_short | Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams |
| title_sort | neospora caninum nc 6 argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams |
| topic | Neospora caninum Ganado Bovino Aborto Técnicas Inmunológicas Inoculación Respuesta Inmunológica Cattle Abortion Immunological Techniques Inoculation Immune Response Argentina |
| url | https://link.springer.com/article/10.1007/s00436-013-3424-1 http://hdl.handle.net/20.500.12123/2329 https://doi.org/10.1007/s00436-013-3424-1 |
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